Mass Spectrometry-Based Disulfide Mapping of Lysyl Oxidase-like 2

Lysyl oxidase-like 2 (LOXL2) catalyzes the oxidative deamination of peptidyl lysines and hydroxylysines to promote extracellular matrix remodeling. Aberrant activity of LOXL2 has been associated with organ fibrosis and tumor metastasis. The lysine tyrosylquinone (LTQ) cofactor is derived from Lys653 and Tyr689 in the amine oxidase domain via post-translational modification. Based on the similarity in hydrodynamic radius and radius of gyration, we recently proposed that the overall structures of the mature LOXL2 (containing LTQ) and the precursor LOXL2 (no LTQ) are very similar. In this study, we conducted a mass spectrometry-based disulfide mapping analysis of recombinant LOXL2 in three forms: a full-length LOXL2 (fl-LOXL2) containing a nearly stoichiometric amount of LTQ, Δ1-2SRCR-LOXL2 (SRCR1 and SRCR2 are truncated) in the precursor form, and Δ1-3SRCR-LOXL2 (SRCR1, SRCR2, SRCR3 are truncated) in a mixture of the precursor and the mature forms. We detected a set of five disulfide bonds that is conserved in both the precursor and the mature recombinant LOXL2s. In addition, we detected a set of four alternative disulfide bonds in low abundance that is not associated with the mature LOXL2. These results suggest that the major set of five disulfide bonds is retained post-LTQ formation.     

A 12-channel phased-array wavelength multiplexer with multimodeinterference couplers

A compact 12-channel phased-array wavelength-division multiplexer based on multimode interference (MMI) couplers and useful for application with multiwavelength lasers is proposed. A suitably-chosen 1×12 MMI nonuniform power splitter is used to suppress the sidelobes of the multiplexer spectral response. Simulated results for the multiplexer designed in an InP-InGaAsP rib waveguide system and with 1-nm channel spacing at a wavelength of 1550 nm are presented. The multiplexer has a length of 3.2 mm and an insertion loss of 1.7 dB     

Beyond design review: collaborating to create well-designed places in Scotland

ABSTRACT

This practice paper reflects on the national design advice service in Scotland that has been operated by Architecture & Design Scotland (A&DS) since 2005. Placed in the context of research on design review practice, the paper describes how design review in Scotland has evolved from a traditional panel model operating alongside a range of training and skills development functions into a more holistic and dynamic design advice service. The paper argues that the evolution of design review in Scotland signifies an innovative shift away from the often combative setting of a design panel, where the force of an argument frequently wins the day, towards a more measured, iterative and collaborative process of nurturing and enabling well-designed buildings and places.     

Rental Discrimination in the Multi-ethnic Metropolis: Evidence from Sydney

Investigating differential treatment in rental housing markets is important to ensure that renters are not discriminated against based on their personal characteristics. However, little Australian research has focused systematically on this question. This paper reports the results of a study that used paired tests to estimate the extent of differential treatment of Anglo, Indian, and Muslim Middle Eastern renters in the Sydney metropolitan housing market. We find statistically significant differences in treatment on several measures, including the likelihood an agent will offer an individual appointment, will provide additional information about other housing, will provide additional information about completing the application form, and will contact a prospective renter after an inspection.     

Student perspectives on mentoring in a science outreach project

Abstract

The focus of this paper is an examination of mentorship relationships developed as a result of six students’ participation in a local community science centre sponsored through an NSERC/CRYSTAL project. Each year, a number of students participate in after‐school activities sponsored by the community science centre. Much of this work involves a one‐to‐one approach, with a middle years or high school student working with a professor partner. The professor acts as a mentor to the student, facilitating learning in a manner not always feasible within a school setting with large class sizes. For some students this research may be the basis of a science fair project; for others if is simply a study based on individual interest. Mentored students were interviewed about the nature of that mentorship, with the goal of describing the experience from the students’ perspective. Questions focused on the student's experience of working with a mentor, including how the mentorship relationship was formed, what the importance of the voluntary nature of mentoring was, what the benefits and challenges of working with a mentor were, how the mentorship relationship affected the level of content knowledge the student gained, and what effect the mentoring relationship had on the student's continuing interest in science. The mentorship program offers students the opportunity to work within a developing community of practice.     

Exploring relationships between satiation,perceived satiety and plant-based snack food features

We aimed to identify relations between satiation and subsequent satiety for six plant-based foods (apple, avocado, banana, carrot, chick pea and macadamia) using a panel of ten healthy participants and a complete crossover randomised design. Food was served as a mid-morning snack ad libitum over 20 min until participants were comfortably full, and subsequent satiety was monitored for up to 180 min. Carrot and macadamia had significantly higher perceived fullness during eating, but also significantly lower perceived satiety per gram than other foods. Food energy factors had no strong relationship with perceived fullness, but were significantly positively correlated with satiety. Mastication number had significant effects on both perceived fullness (negative) and satiety (positive). This pilot study showed that plant food factors affecting perceived fullness during eating and subsequent satiety are different, and suggests how portion control through satiation for different plant-based snacks may influence both energy intake and subsequent satiety.     

Texting While Driving: An Empirical Investigation of Students’ Attitudes and Behaviors

The distracting effects of texting while driving activity of young adults are receiving increasing attention. The findings indicate that individuals involved in texting while driving also engage in other risky behaviors. Furthermore, frequency of texting while driving appears to be related to several factors, including self-control, attitudes toward texting and responsible driving, and opinions of peers, but interestingly not with social responsibility. In conclusion, the frequency of texting while driving of young adults appears to be related to several factors, suggesting possible ways the frequency of texting while driving may be affected.     

Interpersonal goals and change in anxiety and dysphoria in first-semester college students.

Two longitudinal studies examined the associations between interpersonal goals (i.e., self-image and compassionate goals) and anxiety and dysphoria (i.e., distress). In Study 1, 199 college freshmen (122 women, 77 men) completed 12 surveys over 12 weeks. Compassionate goals predicted decreased distress, and self-image goals predicted increased distress from pretest to posttest when distress was assessed as anxiety, dysphoria, or a composite, and when the goals were worded as approach goals, avoidance goals, or a composite. In Study 2, 115 first-semester roommate pairs (86 female and 29 male pairs) completed 12 surveys over 12 weeks. Compassionate and self-image goals predicted distress in same-week, lagged-week, and pretest-to-posttest analyses; effects of compassionate goals remained significant when the authors controlled for several known risk factors. Having clear goals consistently explained the association between compassionate goals but not self-image goals and distress. Results supported a path model in which compassionate goals predict increased support given to roommates, which predicts decreased distress. Results also supported a reciprocal association; chronic distress predicted decreased compassionate and increased self-image goals from pretest to posttest, and weekly distress predicted decreased compassionate goals the subsequent week. The results suggest that compassionate goals contribute to decreased distress because they provide meaning and increase support given to others. Distress, in turn, predicts change in goals, creating the potential for upward and downward spirals of goals and distress. (PsycINFO Database Record (c) 2010 APA, all rights reserved)