Evaluation of a bacteriocin-producing strain of Pediococcus acidilactici as a biopreservative for “Alheira”, a fermented meat sausage

This study was conducted to evaluate the ability of Pediococcus acidilactici HA-6111-2, a PA-1 bacteriocin-producing lactic acid bacterium (LAB), isolated from “Alheira” to inhibit a cocktail of Listeria innocua strains during production and shelf-life of these products. The bacteriocinogenic culture reduced the Listeria population below the detection limit (1.5log CFU/g) and had no effect on the growth of the natural LAB flora or on the pH. Pathogenic organisms were not detected in any sample. The presence of some virulence factors and antibiotic resistances of the strain to be used as a bioprotective culture were investigated. P. acidilactici HA-6111-2 did not produce any of the biogenic amines tested; no formation of biofilms was observed; more l(+)lactic acid was produced than its isomer d(?); no gelatinase, DNase or lipase activity was recorded; no structural genes for the haemolysin, enterococcal surface protein, hydrolytic compounds, aggregation protein and cell-wall adhesins were detected, no significant antibiotic resistances were found. P. acidilactici HA-6111-2 appears to have potential as a bioprotective culture during “Alheira” fermentation. Moreover, a trained panel considered the protected product to be sensorially acceptable.     

Detection of Leakage Freshwater and Friction Factor Calibration in Drinking Networks Using Central Force Optimization

Inverse Transient Analysis (ITA) is a powerful approach for leak detection and calibration of friction factors in pressurized pipes. Through this method, a transient flow is initiated and pressures are measured somewhere in the system. Then, a nonlinear programming (NLP) problem with a least-squares criterion objective function is developed to minimize discrepancies between the measured and calculated pressures at measurement sites. Solving the raised NLP results in the problem’s unknowns being leakage specifications and pipe friction factors. For this purpose, various optimization techniques may be utilized. This issue is a major challenge for ITA-based methods. The present work aims at applying the new method of Central Force Optimization (CFO) to the problem of ITA. CFO is a deterministic metaheuristic inspired by gravitational kinematics in which small objects in space are dragged by bigger ones. Herein, the concept and main structure of CFO are represented as well as of CFO. A reference pipe-network is considered to be solved using the ITA equipped with CFO. The results are then discussed compared to the previous works. It is concluded that CFO is easy to implement, computationally efficient and has a remarkable performance in solving leak detection problem.     

Energy Production in Water Distribution Networks: A PAT Design Strategy

Pump operating as turbine (PAT) is an effective source of reducing the equipment cost in small hydropower plants. However, the manufacturers provide poor information on the PAT performance thus representing a limit for its wider diffusion. Additional implementation difficulties arise under variable operating conditions, characteristic of water distribution networks (WDNs). WDNs allow to obtain widespread and globally significant amount of produced energy by exploiting the head drop due to the network pressure control strategy for leak reductions. Thus a design procedure is proposed that couples a parallel hydraulic circuit with an overall plant efficiency criteria for the market pump selection within a WDN. The proposed design method allows to identify the performance curves of the PAT that maximizes the produced energy for an assigned flow and pressure-head distribution pattern. Finally, computational fluid dynamics (CFD) is shown as a suitable alternative for performance curve assessment covering the limited number of experimental data.     

Rainproof cities in the Netherlands: approaches in Dutch water governance to climate-adaptive urban planning

Abstract

Due to increasingly frequent incidents of pluvial flooding of public spaces and private properties, climate-adaptive building and urban water management are gaining momentum in Dutch water governance. This study assesses the Dutch approach to urban water management by looking at the governance approaches of three of the largest Dutch municipalities: Amsterdam, Rotterdam and Utrecht. By analyzing the municipalities’ governance approaches in a holistic way, paying attention to knowledge, organization and implementation, the research provides good practices in terms of different aspects of resilience as well as lessons regarding setting performance indicators in service levels, clarifying responsibility division, applying binding rules instead of soft policies, and more.     

Stability of the intense sweetener neohesperidine DC during yogurt manufacture and storage

The stability of the intense sweetener neohesperidine dihydrochalcone (DC) has been assessed during yogurt manufacture and storage. No significant decomposition was detected during and after milk pasteurization, the fermentation process and storage for 6 weeks at 3 °C. In addition, the maximum authorized concentration of neohesperidine DC did not affect the rate of acidification by lactic acid bacteria during the fermentation process. Taken together, these results suggest that neohesperidine DC can be added to milk products and will remain stable throughout pasteurization and fermentation processes.     

Dietary procyanidins lower triglyceride levels signaling through the nuclear receptor small heterodimer partner

Hypertriglyceridemia is an independent risk factor in the development of cardiovascular diseases, and we have previously reported that oral administration of a grape seed procyanidin extract (GSPE) drastically decreases plasma levels of triglycerides (TG) and apolipoprotein B (ApoB) in normolipidemic rats, with a concomitant induction in the hepatic expression of the nuclear receptor small heterodimer partner (NR0B2/SHP). Our objective in this study was to elucidate whether SHP is the mediator of the reduction of TG-rich ApoB-containing lipoproteins triggered by GSPE. We show that GSPE inhibited TG and ApoB secretion in human hepatocarcinoma HepG2 cells and had and hypotriglyceridemic effect in wild-type mouse. The TG-lowering action of GSPE was abolished in HepG2 cells transfected with a SHP-specific siRNA and in a SHP-null mouse. Moreover, in mouse liver, GSPE downregulated several lipogenic genes, including steroid response element binding protein 1c (SREBP-1c), and upregulated carnitine palmitoyltransferase-1A (CPT-1A) and apolipoprotein A5 (ApoA5), in a SHP-dependent manner. In HepG2 cells GSPE also inhibited ApoB secretion, but in a SHP-independent manner. In conclusion, SHP is a key mediator of the hypotriglyceridemic response triggered by GSPE. This novel signaling pathway of procyanidins through SHP may be relevant to explain the health effects ascribed to the regular consumption of dietary flavonoids.     

New design solutions for low-power energy production in water pipe systems

This study is the result of ongoing research for a European Union 7th Framework Program Project regarding energy converters for very low heads, and aims to analyze optimization of new cost-effective hydraulic turbine designs for possible implementation in water supply systems (WSSs) or in other pressurized water pipe infrastructures, such as irrigation, wastewater, or drainage systems. A new methodology is presented based on a theoretical, technical and economic analysis. Viability studies focused on small power values for different pipe systems were investigated. Detailed analyses of alternative typical volumetric energy converters were conducted on the basis of mathematical and physical fundamentals as well as computational fluid dynamics (CFD) associated with the interaction between the flow conditions and the system operation. Important constraints (e.g., size, stability, efficiency, and continuous steady flow conditions) can be identified and a search for alternative rotary volumetric converters is being conducted. As promising cost-effective solutions for the coming years, adapted rotor-dynamic turbomachines and non-conventional axial propeller devices were analyzed based on the basic principles of pumps operating as turbines, as well as through an extensive comparison between simulations and experimental tests.     

Valley floor landscape change following almost 100 years of flood embankment abandonment on a wandering gravel‐bed river

Critical to restoring the nature conservation value of many river corridors is an understanding of how alluvial landscapes will respond to cessation of river management and land use practices that have previously degraded the environment. This paper analyses changes in valley floor landforms and vegetation patch dynamics, in relation to fluvial disturbance, over a period of almost 100 years following flood embankment abandonment on a wandering gravel‐bed river, namely the River Tummel, Scotland. Such rivers were once typical of many draining upland areas of northern maritime Europe. Prior to abandonment the valley floor landscape was agriculturally dominated and the river for the most part was single thread confined between flood embankments. The pattern of landform change and vegetation patch development over time following a decision in 1903 not to maintain embankments was tracked by geomorphic and land cover mapping utilizing successive sets of aerial photography for the period 1946 to 1994. A historical context for these changes was also feasible because the channel planform in 1900 and earlier channel planform changes dating back to 1753 were known due to the availability of old maps and earlier geomorphic studies. The land cover mapping was validated by comparison of results produced from the interpretation work on the 1994 aerial photographs with the field‐based UK National Vegetation Classification protocol. The findings of the study illustrate that bordering the River Tummel fluvial landforms and vegetation patch mosaics, presumably resembling those that occurred before valley floor land use intensification, evolved in less than 50 years after flood embankment abandonment with a resultant increase in habitat diversity. The change relates primarily to flood‐induced channel planform change and moderate levels of fluvial disturbance. The general significance of this change to plant species diversity on the valley floor of the River Tummel and elsewhere is discussed as is possible implications of the upstream impoundment and scenarios for climatically induced changes in flood frequency and magnitude. The overall outcome is the strong possibility that simple changes in river management and land use practices could result in re‐establishment of the nature conservation value of similar river corridors in Europe over the medium term without active restoration efforts. Copyright © 2002 John Wiley & Sons, Ltd.     

Atmospheric flows of semi-volatile organic pollutants to the Great Lakes estimated by the United States' Integrated Atmospheric Deposition and Canada's Great Lakes Basin Monitoring and Surveillance Networks

We calculated the wet and dry deposition, vapor absorption, and volatilization flows (in kg/yr) of seven polychlorinated biphenyls (PCBs), nine organochlorine pesticides, and two polycyclic aromatic hydrocarbons (PAHs) into and out of the Great Lakes during 2010–2015 (inclusive). Particle, vapor, and precipitation concentrations from five rural and remote stations (one site on each lake) and two urban sites, operated by the United States and Canada, were used for the flow calculations. Output from the water to the air was the most important process for PCBs, chlordanes, and p,p′-DDE. The flows of endosulfan, p,p′-DDT, and phenanthrene were dominated by vapor absorption from the air to the water. The flow of benzo[a]pyrene was controlled by wet and dry deposition to the water. The flows of the hexachlorocyclohexanes (HCHs) into and out of the lakes were about equal, indicating air-water equilibrium for these compounds. Among the lakes, Lakes Superior and Erie had the highest input and output flows. The input and output flows for the five lakes were decreasing with halving times of 1–10 years and 10–40 years, respectively. Most chemicals had seasonal variations in their flows, with maximum inputs in the summer and maximum outputs in the fall. The flows of PCBs and PAHs into Lakes Michigan and Erie were associated with Chicago and Cleveland, respectively. Combining our data with previous data over the period 1992–2015, we estimated that the input flows of most of these chemicals have significantly decreased, but the output flows do not show consistent trends.     

The AROUND Architecture for Dynamic Location-Based Services

This paper presents a generic concept of location-based service as an abstraction for supporting the association between computational resources and location. The objective is to extend the advantages of service-based architectures to the development of location-based systems, thus providing a more open and extensible alternative to the vertical approaches typically used in this type of system. The novel AROUND architecture is proposed as an approach for supporting location-based services in the Internet environment. AROUND provides a service location infrastructure that allows applications to select services that are specifically associated with their current location. The architecture includes a flexible scope model that defines the association between services and location, and a service location infrastructure organised by spatial criteria and optimised for location-based queries. Based on a prototype implementation of this architecture, we have developed two case studies that illustrate the use of this approach for developing location-based systems. The overall results provide a valuable insight into the applicability of the architecture, and suggest that this model of location-based services can provide a useful approach for the development of a wide range of location-based applications.