Comparison of applied and induced current electrical impedance tomography

Several papers on induced current electrical impedance tomography (IC-EIT) have dwelt on potential advantages of this technique over conventional EIT which uses applied current (AC-EIT). Experimental evidence that IC-EIT could surpass AC-EIT in similar imaging conditions is lacking. In this paper, we describe a system that can switch rapidly between both AC-EIT and IC-EIT. The system makes it possible to image objects in a saline-filled tank, providing data acquired in identical test conditions for comparing the performance of the two modes. The system uses eight circular coils and 16 electrodes to acquire 120 linearly independent measurements in IC-EIT and 104 in AC-EIT. Difference images were reconstructed from data acquired with both modes using the maximum a posteriori method. Spatial resolution was lower in IC-EIT images than in AC-EIT, especially in the radial direction. IC-EIT also exhibits a bias toward the center for positioning a conductivity perturbation. These results were obtained for a typical coil configuration widely used in the literature and may not be representative of alternate coil configurations. The system described in this paper provides stable experimental conditions for comparing the performance of the two EIT imaging modes and would be a valuable tool for validating new coil configurations.     

Retrieval of pigment concentrations and size structure of algal populations from their absorption spectra using multilayered perceptrons

Spectral absorption coefficients of phytoplankton can now be derived, under some assumptions, from hyperspectral ocean color measurements and thus become accessible from space. In this study, multilayer perceptrons have been developed to retrieve information on the pigment composition and size structure of phytoplankton from these absorption spectra. The retrieved variables are the main pigment groups (chlorophylls a, b, c, and photosynthetic and nonphotosynthetic carotenoids) and the relative contributions of three algal size classes (pico-, nano-, and microphytoplankton) to total chlorophyll a. The networks have been trained, tested, and validated using more than 3,700 simultaneous absorption and pigment measurements collected in the world ocean. Among pigment groups, chlorophyll a is the most accurately retrieved (average relative errors of 17% and 16% for the test and validation data subsets, respectively), while the poorest performances are found for chlorophyll b (average relative errors of 51% and 40%). Relative contributions of algal size classes to total chlorophyll a are retrieved with average relative errors of 19% to 33% for the test subset and of 18% to 47% for the validation subset. The performances obtained for the validation data, showing no strong degradation with respect to test data, suggest that these neural networks might be operated with similar performances for a large variety of marine areas.     

General Object Reconstruction Based on Simplex Meshes

In this paper, we propose a general tridimensional reconstruction algorithm of range and volumetric images, based on deformable simplex meshes. Simplex meshes are topologically dual of triangulations and have the advantage of permitting smooth deformations in a simple and efficient manner. Our reconstruction algorithm can handle surfaces without any restriction on their shape or topology. The different tasks performed during the reconstruction include the segmentation of given objects in the scene, the extrapolation of missing data, and the control of smoothness, density, and geometric quality of the reconstructed meshes. The reconstruction takes place in two stages. First, the initialization stage creates a simplex mesh in the vicinity of the data model either manually or using an automatic procedure. Then, after a few iterations, the mesh topology can be modified by creating holes or by increasing its genus. Finally, an iterative refinement algorithm decreases the distance of the mesh from the data while preserving high geometric and topological quality. Several reconstruction examples are provided with quantitative and qualitative results.     

Refractive effects in coherent anti-Stokes Raman scattering microscopy

Coherent anti-Stokes Raman scattering (CARS) microscopy with high sensitivity and high three- dimensional resolution has been developed for the vibrational imaging of chemical species. Due to the coherent nature of the CARS emission, it has been reported that the detection of epi-CARS and forward-CARS (F-CARS) signals depends on the size and shape of the sample. We investigate theoretically and experimentally the effects on the CARS signal of refractive index mismatches between the sample and its surroundings. Backward-CARS and F-CARS signals are measured for different polystyrene bead diameters embedded in different refractive index solvents. We show that index mismatches result in a backward-reflected F-CARS signal that generally dominates the experimentally backward-detected signal. Simulations based on geometrical and wave optics comparing forward- and backward-detected signals for polystyrene beads embedded in different index solvents confirm our findings. Furthermore, we demonstrate that the maxima of forward- and backward-detected signals are generated at different positions along the optical axis in the sample if refractive index mismatches are present between the sample and its surroundings.     

SOCRATE: an optical bench dedicated to the understanding and improvement of a laser conditioning process

We present an automatic excimer laser bench (SOCRATE) allowing for the treatment of optical components by laser conditioning. This apparatus, developed at the Commissariat a l'Energie Atomique-Le Ripault, has been designed to add to this conditioning process an in situ, accurate laser-induced damage threshold (LIDT) measurement and different nondestructive optical techniques for the characterization of the component during treatment. Through different examples, we demonstrate the importance of these characterizations to improve the understanding of the laser conditioning. The role of an in situ adapted metrology associated in real time with a laser conditioning bench offers new opportunities to analyze laser-induced damage mechanisms and subsequently to increase the LIDT of optical components.     

Building maps of local apparent conductivity of the epicardium with a 2-D electrophysiological model of the heart

In this paper, we address the problem of estimating the parameters of an electrophysiological model of the heart from a set of electrical recordings. The chosen model is the reaction-diffusion model on the transmembrane potential proposed by Aliev and Panfilov. For this model of the transmembrane, we estimate a local apparent two-dimensional conductivity from a measured depolarization time distribution. First, we perform an initial adjustment including the choice of initial conditions and of a set of global parameters. We then propose a local estimation by minimizing the quadratic error between the depolarization time computed by the model and the measures. As a first step we address the problem on the epicardial surface in the case of an isotropic version of the Aliev and Panfilov model. The minimization is performed using Brent method without computing the derivative of the error. The feasibility of the approach is demonstrated on synthetic electrophysiological measurements. A proof of concept is obtained on real electrophysiological measures of normal and infarcted canine hearts.     

Squalenoyl nanomedicines as potential therapeutics

Nucleoside analogues display significant anticancer or antiviral activity by interfering with DNA synthesis. However, there are some serious restrictions to their use, including their rapid metabolism and the induction of resistance. We have discovered that the linkage of nucleoside analogues to squalene leads to amphiphilic molecules that self-organize in water as nanoassemblies of 100-300 nm, irrespective of the nucleoside analogue used. The squalenoyl gemcitabine exhibited superior anticancer activity in vitro in human cancer cells and gemcitabine-resistant murine leukemia cells, and in vivo in experimental leukemia both after intravenous and oral administration. The squalenoylation of other antiretroviral nucleosides also led to more potent drugs when tested in primary cultures of HIV-infected lymphocytes. Thus, the squalenoylation is an original technology platform for generating more potent anticancer and antiviral nanomedicines.     

Study of the hydrophobic cavity of beta-cryptogein through laser-polarized xenon NMR spectroscopy

The interaction of xenon with beta-cryptogein, a basic 10 kDa protein belonging to the elicitin family, has been studied by using dissolved thermal and laser-polarized gas in liquid-state NMR. 13C and 1H chemical-shift-mapping experiments were unfruitful, the proton lines only experienced a slight narrowing but no significant frequency variation when the xenon concentration was increased. Nevertheless magnetization transfer from hyperpolarized xenon to protons of the protein demonstrates an undoubted interaction and enables localization of the noble-gas-binding site. Due to the proton-proton cross-relaxation efficiency, however, this experiment is subjected to important spin-diffusion. An automatic procedure that takes spin-diffusion into account when assigning the protons that interact with xenon is then used. The binding site, as defined by 30 Xe--H interactions, is situated in the inner core of the protein. The protons that interact with xenon border the channel by which sterols are known to enter into the cavity. These results support the idea that xenon is a good probe for hydrophobic protein regions.     

Electrochemical boron-doped diamond film microcells micromachined with femtosecond laser: application to the determination of water framework directive metals

Planar electrochemical microcells were micromachined in a microcrystalline boron-doped diamond (BDD) thin layer using a femtosecond laser. The electrochemical performances of the new laser-machined BDD microcell were assessed by differential pulse anodic stripping voltammetry (DPASV) determinations, at the nanomolar level, of the four heavy metal ions of the European Water Framework Directive (WFD): Cd(II), Ni(II), Pb(II), Hg(II). The results are compared with those of previously published BDD electrodes. The calculated detection limits are 0.4, 6.8, 5.5, and 2.3 nM, and the linearities go up to 35, 97, 48, and 5 nM for, respectively, Cd(II), Ni(II) Pb(II), and Hg(II). The detection limits meet with the environmental quality standard of the WFD for three of the four metals. It was shown that the four heavy metals could be detected simultaneously in the concentration ratio usually measured in sewage or runoff waters.     

Fully tunable active polarization imager for contrast enhancement and partial polarimetry

We present the design and the practical implementation of a polarimetric imaging system based on liquid-crystal modulators that allows generation and analysis of any polarization state on the Poincaré sphere. This system is more versatile than standard Mueller imagers that are based on optimized, but limited, sets of illumination and analysis states. Examples of benefits brought by these extra degrees of freedom are illustrated on two different applications: contrast enhancement and extraction of partial polarimetric properties of a scene.