Economic Geography of Knowledge-Intensive Technology Clusters: Lessons from the Helsinki Metropolitan Area

This paper analyzes industrial clusters in the Helsinki Metropolitan Area (HMA) in Finland. The HMA is the largest and most powerful concentration of population and economic activity in Finland. The paper analyzes knowledge-intensive industrial clusters and their structures. Clusters are identified according to a statistical analysis that provides a systematic perspective on the knowledge-intensive economic geography of the HMA. There are two main questions: how diverse are the identified clusters in terms of their internal structure; and, are there spatial irregularities identifiable in these structures? Knowledge-intensive clusters are strongly localized close to the infrastructural nodes: their physical localization is closely linked to road- and rail-structures and terminals. In general, clusters become smaller as their distance to the center of Helsinki increases: distance decay is evidently present. Our findings indicate that clusters are plural entities and their diversities do not follow a clearly identifiable pre-determined logic. Knowledge-based industries focusing on immaterial products tend to have closer central proximity than other industries but variations are extensive. This cluster diversity indicates that the HMA has a threshold for manifesting agglomeration gains that generate and extend industrial diversities within key clusters. The most diverse clusters tend to be located in the urban core, whereas the more narrowly focused clusters may be found in relatively peripheral locations.     

Effect of Quercetin on Paraoxonase 2 Levels in RAW264.7 Macrophages and in Human Monocytes�C�CRole of Quercetin Metabolism

There is increasing evidence that the intracellular antioxidant enzyme paraoxonase 2 (PON2) may have a protective function in the prevention of atherogenesis. An enhancement of PON2 activity by dietary factors including flavonoids is therefore of interest. In the present study we determined the effect of quercetin on paraoxonase 2 levels in cultured murine macrophages in vitro and in overweight subjects with a high cardiovascular risk phenotype supplemented with 150 mg quercetin/day for 42 days in vivo. Supplementation of murine RAW264.7 macrophages in culture with increasing concentrations of quercetin (1, 10, 20 μmol/L) resulted in a significant increase in PON2 mRNA and protein levels, as compared to untreated controls. Unlike quercetin, its glucuronidated metabolite quercetin-3-glucuronide did not affect PON2 gene expression in cultured macrophages. However the methylated quercetin derivative isorhamnetin enhanced PON2 gene expression in RAW264.7 cells to similar extent like quercetin. Although supplementing human volunteers with quercetin was accompanied by a significant increase in plasma quercetin concentration, dietary quercetin supplementation did not change PON2 mRNA levels in human monocytes in vivo. Current data indicate that quercetin supplementation increases PON2 levels in cultured monocytes in vitro but not in human volunteers in vivo.     

Candidate gene association study of type 2 diabetes in a nested case-control study of the EPIC-Potsdam cohort - role of fat assimilation

To search for common variants etiological for type 2 diabetes, we screened 15 genes involved in fat assimilation for sequence variants. Approximately 55 kb in promoter and coding regions, and intron/splice sites were sequenced by cycle sequencing. In the set of 15 genes, 71 single nucleotide polymorphisms (SNPs) were detected. 33 SNPs were presumed to be functionally significant and were genotyped in 192 incident type 2 diabetes subjects and 384 matched controls from the European Prospective Investigation into Cancer and Nutrition-Potsdam cohort. A total of 27 SNPs out of 15 genes showed no statistical association with type 2 diabetes in our study. Six SNPs demonstrated nominal association with type 2 diabetes, with the most significant marker (FABP6 Thr79Met) having an adjusted odds ratio of 0.45 (95% CI 0.22-0.92) in homozygous Met allele carriers. Evidence for an association with disease status was also found for a novel Arg109Cys (g.2129C > T) variant of colipase, 5'UTR (rs2084202) and Met71Val (rs8192506) variants of diazepam-binding inhibitor, Arg298His (rs13283456) of PTGES2, and a novel promoter variant (g.-1324G > A) of SLC27A5. The results presented here provide preliminary evidence for the association of common variants in genes involved in fat assimilation with the genetic susceptibility of type 2 diabetes. However, they definitely need further verification.     

Prostaglandin E synthase 2 (PTGES2) Arg298His polymorphism and parameters of the metabolic syndrome

The prostaglandin E synthase 2 (PTGES2) gene maps to a locus linked to obesity and is involved in the synthesis of the antilipolytic compound prostaglandin E(2). In a recent study, we found an association of the minor PTGES2 Arg298His allele and lower risk of type 2 diabetes mellitus in the European Investigation into Cancer and Nutrition (EPIC) and Cooperative Health Research in the Augsburg Region (KORA) cohorts. Here, we employed our Metabolic Intervention Cohort Kiel (MICK) to assess the influence of the PTGES2 Arg298His polymorphism on a wider scale of parameters of the metabolic syndrome and postprandial metabolism. In comparison to subjects homozygous for the Arg allele, carriers of the His-allele showed significantly lower fasting insulin (geometric mean +/- SEM: 11.8 muU/mL, 11.41-12.25 versus 13.0, 12.71-13.33; p = 0.023), lower postprandial insulin levels after an oral glucose tolerance test (area under the curve 77.2, 74.07-80.52 versus 81.2, 78.8-83.63; p = 0.023) and lower homeostasis model assessment (HOMA)-insulin-resistance (3.030, 2.909-3.157 versus 3.346, 3.257-3.438; p = 0.041) and HOMA-beta-cell-function (107.2, 104.04-110.52 versus 117.2, 114.65-119.71; p = 0.019). Adjustment for body mass index (BMI) resulted in a loss of these significant differences. BMI tended to show lower values in His-allele carriers, (p = 0.067). In conclusion, risk-reducing effects of the minor His allele of the PTGES2 Arg298His polymorphism could be mediated partly by lowered BMI.     

Bestimmung von Polydextrose in Lebensmitteln mittels Ionenchromatographie und gepulster amperometrischer Detektion

Zusammenfassung Beschrieben wird die flüssigchromatographische Bestimmung des synthetischen Polysaccharids Polydextrose, das zur Herstellung von kalorienreduzierten Lebensmitteln verwendet wird. Die sehr einfache Probenaufarbeitung beinhaltet die wäßrige Extraktion des Analyten und den enzymatischen Abbau weiterer wasserlöslicher Polysaccharide wie Stärke und Inulin. Das chromatographische System besteht aus einer Anionenaustauscher-Säule, die Kohlenhydrate bis zu einem Molekulargewicht von 15000 zu trennen vermag und einem gepulsten amperometrischen Detektor (PAD), mit dem Saccharide underivatisiert selektiv und empfindlich nachgewiesen werden können. Die Wiederfindungsraten von Polydextrose in verschiedenen Süßwaren betrugen zwischen 94 und 106% und bei Backwaren 100 bis 115%.

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Determination of the synthetic carbohydrate Polydextrose

Summary A method to determine the synthetic carbohydrate Polydextrose, which is used as a bulking agent for the preparation of calorie-reduced foodstuffs, is described. The sample clean-up involves the extraction of Polydextrose with water and enzymatic degradation of interfering polysaccharides, such as starch and inulin. The chromatographic system consists of a high-performance anion-exchange column that separates carbohydrates up to molecular weights of 15,000 and pulsed amperometric detection. For unbaked goods recoveries ranged from 94 to 106%, for baked goods recovery rates of 100 to 115% were measured.