Improve the quality of vaginal delivery]

Between 1990 and 1992, external sphincter repair was performed in 20 patients rendered faecally incontinent after earlier fistula surgery. All were improved, 13 (65%) achieving Grade 1 or 2 continence. Postoperative ultrasound was useful in explaining the sub-optimal outcome of 3 patients who still had defects. Clinical outcome in female patients was better than in male patients. Clinical outcome was not related to the results of preoperative manometry, the site of external sphincter damage, the use of a stoma, or the occurrence of wound complications. External sphincter repair for faecal incontinence after fistula surgery achieves good results in the majority of patients.     

Artefactual cleavage of E coli H-NS by OmpT

In the bacterium Escherichia coli, H-NS-(H1, H1a) is a heat-stable protein with a molecular mass of 15.5 kDa involved in nucleoid organisation and gene regulation linked to certain signal transduction pathways. We have shown that, following addition of preparations of everted inner membrane vesicles, heat-stable cleavage products of approximately 10 kDa of H-NS are formed in vitro from newly synthesised, radio-labelled H-NS and from purified H-NS. The 15.5 kDa protein and its cleavage products were also recovered from a minicell system. These results raised the possibility that cleavage of H-NS is physiologically significant. However, the cleavage of H-NS observed appears to occur during cell breakage and to depend on the method of protein extraction and the presence of the outer membrane protease, OmpT. Nevertheless, the results indicate that H-NS may contain at least two separate domains with cleavage occurring between these domains at a preferred OmpT site. Failure to take account of H-NS cleavage in sample preparation and analysis can lead to serious underestimation of H-NS levels.     

Modulation of cAMP-dependent protein kinase by derivatives of chondroitin sulfate

Here, we report investigations about the direct effect of glycosaminoglycans, such as dermatan sulfate, chondroitin 4- and 6-sulfate upon cAMP-dependent protein kinase activity. The results indicate that glycosaminoglycans strongly influence the phosphorylation activity of this enzyme against histone type IIa and [Val6, Ala7]-kemptide. While chondroitin 4-sulfate and dermatan sulfate exhibit inhibitory effects, chondroitin 6-sulfate shows a stimulating effect. In addition, the chondroitin 6-sulfate is also able to reduce the chondroitin 4-sulfate and dermatan sulfate specific inhibition.     

Study of the nutrient distribution in root tips of the tropical forage Brachiaria

A Brachiaria breeding project is being conducted at CIAT, Colombia to combine acid soil adaptation of B. decumbens with other favourable traits of Brachiaria species that are less adapted to acid soils. Micro-PIXE was applied to investigate nutrient uptake and distribution in root tips of different species grown in hydroponic culture under control and simulated acid soil stress conditions. Different sample measurement approaches were evaluated, including: (1) linear scans with single point measurements along the root axis; (2) mapping of whole root tips; and (3) mapping of root cross sections. Different tissue types could be distinguished on the base of differences in nutrient concentrations and/or Al stress. Al, if supplied under nutrient stress conditions, increased P accumulation in the central vascular tissue of the meristematic and elongation zone of B. decumbens and B. brizantha. Furthermore, a negative correlation was found between Al and Cl accumulation in the root cap of B. decumbens.     

Effects of ACh on the electric activity of rostral ventrolateral medullary neurons of rat in vitro

Extracellular single-unit discharges were obtained from 165 spontaneously active neurons within the region of the rostral ventrolateral medulla (RVLM) by glass microelectrode from 89 brain slices of the Sprague-Dawley rats. The units could be divided into three types: regular (61.8%), irregular (24.2%) and silent (14%). Acetylcholine (ACh, 0.1, 0.3 mumol/L) showed four kinds of effects on spontaneous discharges of RVLM neurons: excitatory, inhibitory, biphasic and non-responsive, counting respectively 41.8%, 20%, 3% and 35.2% of the neurons tested. The excitatory effect of ACh was dose-dependent. The effects, either excitatory or inhibitory, of ACh (n = 49) were mostly blocked by atropine (0.3 mumol/L, n = 42). The excitatory effect of ACh (n = 14) could be blocked mainly by selective antagonist of M1 receptor, pirenzepine (PZ, 30 nmol/L, n = 9), but not by selective antagonist of M2 receptor, methoctramine (MT) and AFDX-116. The inhibitory effect of ACh (n = 10) could be blocked mostly by M2 receptor antagonist MT (30 nmol/L, n = 7); and this inhibitory effect (n = 9) could be blocked mostly by another M2 receptor antagonist AFDX-116 (30 nmol/L, n = 6), but not by M1 receptor antagonist PZ.     

Polynomial-complexity deadlock avoidance policies for sequentialresource allocation systems

The development of efficient deadlock avoidance policies (DAPs) for sequential resource allocation systems (RASs) is a problem of increasing interest in the scientific community, largely because of its relevance to the design of large-scale flexibly automated manufacturing systems. Much of the work on this problem existing in the literature is focused on the so-called single-unit RAS model, which is the simplest model in the considered class of RASs. Furthermore, due to a well-established result stating that, even for single-unit RASs, the computation of the maximally permissive DAP is intractable (NP-hard), many researchers (including our group) have focused on obtaining good suboptimal policies which are computationally tractable (scalable) and provably correct. In the first part of the paper, it is shown, however, that for a large subset (in fact, a majority) of single-unit RASs, the optimal DAP can be obtained in real-time with a computational cost which is a polynomial function of the system size (i.e., the number of resource types and the distinct route stages of the processes running through the system). The implications of this result for the entire class of single-unit RASs are also explored. With a result on the design of optimal DAPs for single-unit RASs, the second part of the paper concentrates on the development of scalable and provably correct DAPs for the more general case of conjunctive RASs     

Infinite-dimensional convex optimization in optimal and robustcontrol theory

Many engineering problems can be shown to be equivalent to solving semidefinite programs (SPs), i.e., convex optimization problems involving linear matrix inequalities (LMIs). Powerful computation tools are available for such problems in the finite-dimensional case. However, the problems arising in optimal and robust control theory are often infinite dimensional, and so adequate computation tools are not available. The key to tackling such problems with finite computation tools is to have a primal-dual formulation of the problem without duality gap. In this paper we study infinite-dimensional SPs and present a lifting technique to recast SPs as parameterized linear programs (LPs). This enables the wealth of theoretical tools available for infinite-dimensional LPs to be extended to infinite-dimensional SPs. In particular, we develop some new sufficient conditions for the lack of a duality gap for infinite-dimensional SPs and give an exact characterization of the primal and dual problems for these cases. Both primal and dual problems are formed as infinite-dimensional SP problems, with finite truncations to each giving upper and lower bounds, respectively, on the exact solution to the infinite-dimensional problem. Thus, these results can form the basis of practical computation schemes for infinite-dimensional problems, which require only finite-dimensional computation tools. To illustrate the power of these tools we apply the results to two previously unsolved optimization problems, namely minimizing the l₁ norm of a closed-loop system subject to bounds on the frequency response magnitude at a finite number of points and/or bounds on the H₂ norm     

New advances on a posteriori error on constitutive relation in f.e. analysis

This paper deals with error estimators based on residuals on the constitutive relation which have been developed for the past 20 years at Cachan. Especially in the case of elasticity, these error estimators can become poor in situations where the material and the mesh are strongly anisotropic. The crucial point herein is the construction of equilibrated stress fields from the computed finite element solution. Therefore, in this paper, the approach and in particular this last point are re-examined. Several important modifications are introduced in the concepts and basic techniques. Hence, a new generation of error estimators which seem to be extremely robust upon initial applications has been generated.