One-sample pregnancy diagnosis in elk using fecal steroid metabolites

In utero hypoxia may affect the development of the brain and result in altered respiratory responses postnatally. Using a barometric plethysmograph, we examined the effects of exposing pregnant guinea pigs to 200 ppm carbon monoxide (CO) for 10 h/d from d 23-25 of gestation until term (approximately 68 d) on the ventilatory responses of their 4-5-d-old neonates at rest, and during progressive asphyxia and steady state hypercapnia. Exposure to this concentration of CO produced significantly higher levels of carboxyhemoglobin (COHb) in maternal (8.53 +/- 0.6% versus 0.25 +/- 0.1%) and fetal blood (13.0 +/- 0.4% versus 1.6 +/- 0.1%) from CO-treated animals when compared with controls. Hematocrit was significantly higher in the CO-treated neonates (46.3 +/- 1.0% versus 41.3 +/- 0.9%) at 5-6 d of age, although no difference existed between the groups for COHb at this time. There was no difference between the groups for length of gestation, litter size, or birth weight, but CO-treated neonates were significantly smaller at 4 d of age (102.4 +/- 3.7 g) compared with controls (132.0 +/- 5.0 g). At 4-5 d of age there was no difference between the groups for either tidal volume (VT), respiratory frequency (f), or minute ventilation (VE) at rest, but during steady state hypercapnia (4 and 6% CO2) the CO-treated neonates had a significantly greater VT and VE (but not f) than did controls. During progressive asphyxia, CO-treated animals had a significantly greater VT than did controls from 1-8% CO2. There was a significant fall in f at 1 and 3% CO2 in CO-treated animals; however, this effect did not persist, resulting in a significantly increased VE from 3 to 8% CO2. The inspiratory flow rate (VT/expiratory time) was significantly increased in the CO-treated neonates during progressive asphyxia; this occurred in the absence of a difference in inspiratory time between the groups. These results indicate that prenatal exposure to CO increases CO2 sensitivity in 4-5-d-old guinea pigs. This may be due to developmental alterations in the areas of the brainstem responsible for respiratory control.     

Gamma-spectroscopy investigation of radon daughter deposition on electrostatically charged surfaces

The effect of static electricity on the deposition of radon daughters onto charged surfaces is determined by a combined experimental and theoretical analysis. Experiments with charged surfaces exposed to the air in a normal working environment are analysed to determine an empirical radon daughter deposition rate. This factor is utilized to estimate the daughter deposition on a human head which is exposed to similar conditions of air quality and static charging. The results indicate that typical levels of static electricity can enhance the deposition of radon daughters by orders of magnitude compared with the uncharged condition. The corresponding yearly alpha dose equivalents to the basal skin layer and to the eye exceed recommended limits. Beside having an important impact from the public health perspective, these results suggest that the obscure and contradictory correlations found between radon concentrations and adverse health effects may arise from a failure to account for the effects of static electricity.     

Potassium currents in motoneurones

The measurements of intrinsic unitary and macroscopic properties of potassium channels in motoneurones constitutes an important step in the characterization of cellular function. A number of potassium currents contribute to the integrated function of motoneurones and this review considers the properties of the inward rectifier (Ih-type), calcium-dependent (BK- and SK-types), delayed rectifier, transient outward, and leak currents. The modification of channel properties by agents such as serotonin and thyrotropin-releasing hormone is likely relevant to modulation of motoneurone function. Indeed, even during the course of normal cell development, the expression and properties of particular potassium channels can undergo considerable changes, which would be manifested as altered firing patterns and excitability of motoneurones. Brainstem and spinal cord slices have considerable utility for electrophysiological studies and have been used in recordings from spinal, hypoglossal and facial motoneurones. New procedures have also been developed which would enhance the purification of cultured motoneurones for patch-clamp experiments.     

Regression of childhood Barrett's esophageal mucosa by antireflux surgery and bipolar electrocoagulation

The authors report a case of a 13-year-old girl with Barrett's esophagus who underwent antireflux surgery and was subsequently treated with endoscopic thermal coagulation using bipolar electrocoagulation. Follow-up endoscopy 15 months after completion of the endoscopic therapy showed normal esophageal mucosa without intestinal metaplasia. Longer follow-up is needed to assess the long-term effects of endoscopic treatment of the Barrett's mucosa with thermal coagulation, and this procedure should still be considered under investigation.