Antibody- and cell-mediated immune responses of Actinobacillus pleuropneumoniae-infected and bacterin-vaccinated pigs

The number involved in and the rate of migration of donor leucocytes into the following recipient organs (spleen, thymus, bone marrow, lung and mesenteric lymph nodes) were measured in two rat models of orthotopic liver transplantation (OLT) using donor-specific MHC class I antibodies. The first OLT model is one that does not require immunosuppression in order to achieve tolerance and involved the transplantation of DA (MHC haplotype, RT1a) livers into PVG (RT1c) recipients. The second model was one that required a 7-day (10 mg/kg) treatment with cyclosporin A (CsA) to achieve tolerance and used DA donors into Lewis (RT1(1)) recipients. Recipient organs were biopsied on days 3, 20 and 87 following OLT and donor leucocyte migration was quantified by immunohistochemistry and computer densitometry of immunoblots of detergent-solublized tissues in order to resolve both membrane-bound and soluble donor MHC class I antigen. In a separate experiment, spleen biopsies were taken following OLT on days 3 and 15 from the naturally tolerizing OLT model (DA into PVG), treated with and without CsA for 7 days and compared with the (DA into Lewis) model. The initial rate of leucocyte migration between days 3 and 21 following OLT was found to be the most rapid into the spleen, followed by the bone marrow and mesenteric lymph nodes in the naturally tolerant (DA into PVG) model when compared with the (DA into Lewis) model. The number of donor leucocytes in the spleen and mesenteric lymph nodes in both models was, however, approximately the same by 87 days. No real difference in the rate of leucocyte migration was seen in the thymus or the lung for both transplant models over the time course assayed. CsA was found to lower the rate of donor leucocyte migration only over the period it was administered. The rate of donor leucocyte migration into the spleen was still much lower 15 days after OLT in the (DA into Lewis) model compared with the (DA into PVG) model treated with and without CsA. Thus the differences in the rate of donor leucocyte migration into the spleen, bone marrow and mesenteric lymph nodes immediately following OLT may offer an explanation as to why the (DA into PVG) combination is able to accept a transplanted liver without immunosuppressive therapy.     

水淬渣罐铸造中支臂及支轴的热节问题分析

分析了钢渣水淬渣罐铸造过程中存在的支臂部位热节处理和支轴部位相对尺寸铸造定位问题。通过改造支臂结构,分散了集中于支臂部位的热节;利用特种模型材料和对支轴的准确定位,解决了支轴部位的相对尺寸精度问题。     

耐热钢主蒸汽管的焊接

1前言我厂动力区三期锅炉项目为2台75 t/h和1台130 t/h锅炉,与之配套的主蒸汽管道大量使用不同规格的15CrMo耐热管材,其主要规格为D273mm×11mm、D219mm×10mm、D159mm×9mm、D76mm×6mm、D36mm×4mm,管内蒸汽工作温度为450℃,工作压力为3.82 MPa,根据DL5007—92《电力建设施工及验收技术规范(》火力发电厂焊接篇)的要求,50%探伤,焊后24 h进行X射线探伤,评定标准为SD143—85,Ⅱ级合格。为保证质量,对15CrMo钢的焊接工艺特点进行了分析,评定了焊接工艺。2焊接工艺特点2、1 15CrMo属耐热钢,一般在热处理状态下焊接,其热处理状态为900…     

基于Web Services的水调自动化系统

在阐述水调自动化系统主要应用功能及介绍Web Services技术的基础上,结合WebServices的原理及实现机制提出了面向网络服务的水调自动化系统设计方案.介绍了该系统的总体结构、实现思路及其技术优势和不足,为水调自动化系统的研制与开发提供了一个新型、可靠的设计方案.     

上犹江水电站大坝闸墩与廊道顶拱裂缝成因分析

针对江西上犹江水电站大坝闸墩裂缝和检查廊道顶拱的贯穿裂缝,运用温度场和温度应力的有限元方法对施工和运行过程进行模拟计算,在运行期重点考虑夏季泄洪冷击和冬季寒潮对开裂的影响,根据计算结果对裂缝的成因进行分析。计算结果表明,不同时期的温度应力是产生裂缝的主要原因。提出了裂缝处理和预防的建议。     

A role for Rev in the association of HIV-1 gag mRNA with cytoskeletal beta-actin and viral protein expression

Human immunodeficiency virus type-1 (HIV-1) Rev acts by inducing the specific nucleocytoplasmic transport of a class of incompletely spliced RNAs that encodes the viral structural proteins. The transfection of HeLa cells with a rev-defective HIV-1 expression plasmid, however, resulted in the export of overexpressed, intron-containing species of viral RNAs, possibly through a default process of nuclear retention. Thus, this system enabled us to directly compare Rev+ and Rev+ cells as to the usage of RRE-containing mRNAs by the cellular translational machinery. Biochemical examination of the transfected cells revealed that although significant levels of gag and env mRNAs were detected in both the presence and absence of Rev, efficient production of viral proteins was strictly dependent on the presence of Rev. A fluorescence in situ hybridisation assay confirmed these findings and provided further evidence that even in the presence of Rev, not all of the viral mRNA was equally translated. At the early phase of RNA export in Rev+ cells, gag mRNA was observed throughout both the cytoplasm and nucleoplasm as uniform fine stippling. In addition, the mRNA formed clusters mainly in the perinuclear region, which were not observed in Rev+ cells. In the presence of Rev, expression of the gag protein was limited to these perinuclear sites where the mRNA accumulated. Subsequent staining of the cytoskeletal proteins demonstrated that in Rev+ cells gag mRNA is colocalized with beta-actin in the sites where the RNA formed clusters. In the absence of Rev, in contrast, the gag mRNA failed to associate with the cytoskeletal proteins. These results suggest that in addition to promoting the emergence of intron-containing RNA from the nucleus, Rev plays an important role in the compartmentation of translation by directing RRE-containing mRNAs to the beta-actin to form the perinuclear clusters at which the synthesis of viral structural proteins begins.     

改良可逆性局灶性脑缺血模型建立

目的改良大鼠可逆性大脑中动脉缺血模型的栓子制作方法,增加模型稳定性和成功率,提高脑缺血实验研究结果的可靠性.方法体重280～320g Wistar雄性大鼠60只,随机分成改良组和对照组,每组30只.对照组用尼龙线一端加热法制作传统栓子,改良组用尼龙线一端沾silicone胶法制作改良栓子.两组模型除栓子制作方法不同外,其他手术步骤完全相同.采用Zea Longa 5分制法进行模型的神经功能评分,采用TTC染色法测量并计算梗死灶的大小.比较两组模型制作成功率、成功模型中神经功能评分3、4级大鼠所占百分数,以     

应力、强度呈非正态分布时可靠度公式的推导

介绍一种当应力、强度呈非正态分布时的可靠度的计算方法,以用来进行机械可靠性设计,并以一个地震载荷的直立压力容器为例加以说明。