Tumor-Promoting Actions of HNRNP A1 in HCC Are Associated with Cell Cycle,Mitochondrial Dynamics,and Necroptosis

Hepatocellular carcinoma (HCC) is one of the most frequent malignancies in the world. Although increasing evidence supports the role of heterogeneous ribonucleoprotein particle A1 (HNRNP A1) in tumor progression, the function of HNRNP A1 in HCC remains unclear. Here, we focused on the role of HNRNP A1 in the development of HCC. In this study, we found HNRNP A1 participates in many aspects of HCC, such as progression and prognosis. Our results showed that HNRNP A1 is upregulated in human HCC tissues and cell lines. High expression of HNRNP A1 can promote the proliferation, migration, and invasion in HCC cells and accelerate tumor progression in mice. Moreover, we found that HNRNP A1 prevents the senescence process of HCC cells. Knocking down of HNRNP A1 promotes the expression of P16^INK4, which arrests the cell cycle and then induces the senescence phenotype in HCC cells. Furthermore, we found that HNRNP A1 regulated necroptosis and mitochondrial dynamics. In summary, our study indicates that HNRNP A1 promotes the development of HCC, which suggests a potential therapeutic target for HCC.     

Precision Killing of Sinoporphyrin Sodium-Mediated Photodynamic Therapy against Malignant Tumor Cells

Photodynamic therapy (PDT) has significant advantages in the treatment of malignant tumors, such as high efficiency, minimal invasion and less side effects, and it can preserve the integrity and quality of the organs. The power density, irradiation time and photosensitizer (PS) concentration are three main parameters that play important roles in killing tumor cells. However, until now, the underlying relationships among them for PDT outcomes have been unclear. In this study, human malignant glioblastoma U-118MG and melanoma A375 cells were selected, and the product of the power density, irradiation time and PS concentration was defined as the total photodynamic parameter (TPP), in order to investigate the mechanisms of PS sinoporphyrin sodium (DVDMS)-mediated PDT (DVDMS-PDT). The results showed that the survival rates of the U-118MG and A375 cells were negatively correlated with the TPP value in the curve, and the correlation exactly filed an e-exponential function. Moreover, according to the formula, we realized controllable killing effects of the tumor cells by randomly adjusting the three parameters, and we finally verified the accuracy and repeatability of the formula. In conclusion, the establishment and implementation of a newly functional relationship among the PDT parameters are essential for predicting PDT outcomes and providing personalized precise treatment, and they are contributive to the development of PDT dosimetry.     

热激处理减轻黄瓜冷害与细胞壁代谢的关系

为了进一步阐明热激处理缓解黄瓜冷害症状的作用机理,研究热激处理对采后黄瓜果实冷害指数、细胞壁组分以及细胞壁降解酶活性的影响,将\     

基于本体的隧道盾构过程中地表沉降的风险因素推理与应对

建筑信息模型(BIM)为传统建筑业的信息化变革带来大量技术革新,可优化建筑过程并提升建筑效率与效益。在行业应用中,围绕建筑全生命周期不同阶段的异构软件间存在诸多异构信息系统与数据交互问题,本文通过分析目前建筑行业信息交互现状,提出基于本体的异构数据交互模式,并阐述IFC数据模型、异构数据库和本体模型间的映射方法及基于事件的本体推理模式。本文针对隧道盾构施工中的地表沉降,通过Protégé酌软件构建地下隧道施工本体,并利用Jena推理机分析地表沉降的关联风险因素,并通过异构数据互用与交互实现地表沉降应对方案的提供与更新。     

1-MCP处理对鸭梨常温贮藏品质及生理指标的影响

以鸭梨果实为试材,采用1.0μL/L的1-MCP处理果实,研究了1-MCP处理对常温贮藏鸭梨果实品质及生理指标的影响。研究结果表明:与对照相比,1-MCP处理显著（p<0.05）降低了鸭梨常温贮藏的呼吸强度和乙烯释放量,推迟了呼吸和乙烯高峰的出现,抑制了果实PPO酶活性,延缓了果实可溶性固形物含量和硬度的下降。1-MCP处理较好地提高了鸭梨常温贮藏的好果率,并延长了贮藏时间。      

课堂教学-实验-课外科技活动三位一体提升硬件类课程质量

本文结合本校硬件类课程教学改革中的体会和经验,提出通过课堂教学-实验-课外科技活动三者有机结合,依靠学科知识竞赛、校企合作等形式,提高硬件类课程教学质量的教学改革方法。     

深度双重注意力的生成与判别联合学习的行人重识别

在行人重识别任务中存在数据集标注难度大,样本量少,特征提取后细节特征缺失等问题。针对以上问题提出深度双重注意力的生成与判别联合学习的行人重识别。首先,构建联合学习框架,将判别模块嵌入生成模块,实现图像生成和判别端到端的训练,及时将生成图像反馈给判别模块,同时优化生成模块与判别模块。其次,通过相邻的通道注意力模块间连接和相邻空间注意力模块间连接,融合所有通道特征和空间特征,构建深度双重注意力模块,将其嵌入教师模型,使模型能更好地提取行人细节身份特征,提高模型识别能力。实验结果表明,该算法在Market-1501和DukeMTMC-ReID数据集上具有较好的鲁棒性、判别性。     

A Novel Human Antibody,HF, against HER2/erb-B2 Obtained by a Computer-Aided Antibody Design Method

Fully human antibodies have minimal immunogenicity and safety profiles. At present, most potential antibody drugs in clinical trials are humanized or fully human. Human antibodies are mostly generated using the phage display method (in vitro) or by transgenic mice (in vivo); other methods include B lymphocyte immortalization, human–human hybridoma, and single-cell polymerase chain reaction. Here, we describe a structure-based computer-aided de novo design technology for human antibody generation. Based on the complex structure of human epidermal growth factor receptor 2 (HER2)/Herceptin, we first designed six short peptides targeting the potential epitope of HER2 recognized by Herceptin. Next, these peptides were set as complementarity determining regions in a suitable immunoglobulin frame, giving birth to a novel anti-HER2 antibody named “HF,” which possessed higher affinity and more effective anti-tumor activity than Herceptin. Our work offers a useful tool for the quick design and selection of novel human antibodies for basic mechanical research as well as for imaging and clinical applications in immune-related diseases, such as cancer and infectious diseases.