Technical Validation and Clinical Implications of Ultrasensitive PCR Approaches for EGFR-Thr790Met Mutation Detection in Pretreatment FFPE Samples and in Liquid Biopsies from Non-Small Cell Lung Cancer Patients

In pretreatment tumor samples of EGFR-mutated non-small cell lung cancer (NSCLC) patients, EGFR-Thr790Met mutation has been detected in a variable prevalence by different ultrasensitive assays with controversial prognostic value. Furthermore, its detection in liquid biopsy (LB) samples remains challenging, being hampered by the shortage of circulating tumor DNA (ctDNA). Here, we describe the technical validation and clinical implications of a real-time PCR with peptide nucleic acid (PNA-Clamp) and digital droplet PCR (ddPCR) for EGFR-Thr790Met detection in diagnosis FFPE samples and in LB. Limit of blank (LOB) and limit of detection (LOD) were established by analyzing negative and low variant allele frequency (VAF) FFPE and LB specimens. In a cohort of 78 FFPE samples, both techniques showed an overall agreement (OA) of 94.20%. EGFR-Thr790Met was detected in 26.47% of cases and was associated with better progression-free survival (PFS) (16.83 ± 7.76 vs. 11.47 ± 1.83 months; p = 0.047). In LB, ddPCR was implemented in routine diagnostics under UNE-EN ISO 15189:2013 accreditation, increasing the detection rate of 32.43% by conventional methods up to 45.95%. During follow-up, ddPCR detected EGFR-Thr790Met up to 7 months before radiological progression. Extensively validated ultrasensitive assays might decipher the utility of pretreatment EGFR-Thr790Met and improve its detection rate in LB studies, even anticipating radiological progression.     

Improving the Management of Endometrial Cancer Patients through the Use of Liquid Biopsy Analyses: A Case Report

Endometrial cancer (EC) is the 4th most common neoplasm of the female genital tract, with 15–20% of patients being of high risk of recurrence which leads to a significant decrease in patient survival. Current therapeutic options for patients with EC are poor, being the combined therapy of carboplatin and paclitaxel the standard of care, with limited efficacy. Therefore, new therapeutic options and better monitoring tools are needed to improve the management of the disease. In the current case report, we showcase the value of liquid biopsy analyses in a microsatellite instability EC patient with initially good prognosis that however underwent rapid progression disease within 6 months post-surgery; through the study of plasma cfDNA/ctDNA dynamics to assess the tumour evolution during treatment, as well as the study of the uterine aspirate as a valuable sample that captures the intra-tumour heterogeneity that allows a comprehensive genomic profiling of the disease to identify potential therapeutic options. Furthermore, preclinical models were generated at the time of tumour progression to assess the efficacy of the identified targeted therapies.     

一种新的基于分层分组式结构的组播密钥管理方案

分析了常见的几种分层分布式密钥管理方案,并且在前人的基础上提出了一种新的基于分层分布式结构的组播密钥管理构想,介绍了这种方案的基本思想。通过实验分析比较了这种方案与前人方案的不同点及相同点,以及各自的优缺点。     

小型无人直升机的纵向横向解耦优化控制

论文根据小型无人直升机的机理建立了数学模型,并在悬停状态下近似线性化,运用Matlab的非线性控制工具箱(NCD)设计了具有很强鲁棒性的控制器,实现了对于小型无人直升机的纵向和横向的解耦控制,仿真结果证明了方法的有效性。     

城市人防警报监控系统软件设计和实现

主要介绍用VB实现无线通信的软件设计。并且提出了使系统应用程序更有效和可靠的方法。     

BaCe0.5-xZr0.5-xYb2xO3-δ质子导体的制备及电性能

采用固相法制备了BaCe0.5-xZr0.5-xYb2xO3-δ(2x=0.1,0.15,0.2,0.25)固体电解质。X射线衍射(XRD)谱图分析结果表明,材料为立方钙钛矿结构。利用直流四探针法测定不同温度和不同气氛下材料的电导率,BaCe0.5-xZr0.5-x-Yb2xO3-δ的电导率与氧空位以及固溶度有关。在空气气氛下材料以电子空穴和氧离子电导为主,而在氢气气氛下,表现为质子导体。当2x=0.2时,在湿氢气气氛下于950℃的电导率为3.6×10-3S/cm。     

关于提高高职专业英语学生语言能力的思考

长期以来,我国高职英语教育培养目标为基于国内中小企业的实际应用型人才.相较普通本科专业英语重基础而言,学生的英语综合素质低,理论基础不扎实,已经严重影响到高职英语专业学生的就业竞争力.如何提高高职英语学生的语言能力成为高专英语教育的一个关键问题.拟从英语教育的四个基本能力--听、说、读、写方面探讨,试图探索出适合高专英语语言教育的新途径.     

Optimization of the transmission mechanism for wireless TCP

A novel method which can detect the possible handoffs for mobile nodes is proposed in this article. The method can be deployed on mobile nodes. So when a possible handoff occurs, mobile nodes can detect and take some necessary actions in time. In this study, the method is also used to optimize the transmission mechanism of Q-TCP. Moreover, the simulation results show that Q-TCP can provide better quality of service （QoS） for mobile nodes when they are moving among different agents.     

Realizing simultaneously enhanced energy and power density fullcell construction using mixed hard carbon/Li_4Ti_5O_(12) electrode

Practical applications of lithium-ion batteries(LIBs) with both high energy and power density are urgently demanded,which require suitable charge/discharge platform,fast charge-transfer kinetics,as well as optimal solid electrolyte interphase(SEI) layer of electrode materials.In this work,a high-performance lithium-ion battery(LIB) full cell was assembled by using commercial LiNi_(0.33)Co_(0.33)Mn_(0.33)O_2(NCM111) as the positive electrode and mixed Li_4 Li_5 O_(12)(LTO)/hard carbon(HC) as the negative electrode.It reveals that the component ratio between LTO and HC plays a critical role in manipulating the electric conductivity and the electro-reaction platform.The electrochemical test results show that when the content of HC is 10 wt%,the as-constructed full cell demonstrates the best electrochemical,with a maximum energy density of 149.2 Wh·kg~(-1) and a maximum power density of2195 W·kg~(-1) at 10 A·g~(-1)(30 C).This outperforms all the assembled systems within our work range and the state-ofthe-art literatures.The NCM//Li_4 Ti_5 O_(12)+10 wt% HC battery system also exhibits a good capacity retention after1000 cycles at the current density of 1 A·g~(-1).This work provides a new approach to enhance the full-cell performance by mixing electrode materials with different charge potentials and reaction kinetics.     

我国小城镇发展中的增长管理研究

美国较早经历了城市无限制增长的危害,因而也较早进行了相应的公共活动并取得了一定的效果,本文介绍了美国增长管理概念,并把城市增长管理与我国的小城镇发展相结合,针对我国小城镇发展中出现的问题,探讨了如何对发展中的小城镇进行管理,以实现小城镇的可持续发展。