Monoclonal antibody-based competitive ELISA for simultaneous detection of rinderpest virus and peste des petits ruminants virus antibodies

An experimental competitive enzyme-linked immunosorbent assay (morbillivirus cELISA) using a recombinant N antigen (rRPV N) expressed in a baculovirus and a ruminant morbillivirus (RPV and PPRV)-specific monoclonal antibody (P-13A9) was developed for simultaneous detection of rinderpest virus (RPV) and peste des petits ruminants virus (PPRV) antibodies and its diagnostic performance was evaluated. A set of known reference antisera against RPV and PPRV belonging to different lineages, experimental sera from cattle vaccinated for a RPV of Asian lineage, and field sera from cattle and sheep/goat populations known to be positive (West Africa) and negative (Korea) for RPV and PPRV were used for the evaluation. Morbillivirus cELISA results on the panel of experimental RPV and PPRV antisera showed high correlation (r=0.97) between the whole virus and the rRPV N antigens, suggesting that the rRPV N contains a ruminant morbillivirus-specific antigenic determinant recognized by the P-13A9 and it may be suitable as an ELISA antigen in place of the whole virus. Morbillivirus cELISA detected anti-RPV and anti-PPRV antibodies in all reference RPV and PPRV antisera containing VN titers >/=1:8, suggesting that the assay can simultaneously detect antibodies against RPV and PPRV. Anti-RPV antibody was detected by morbillivirus cELISA in vaccinated cattle as early as the VNT and continued to be detectable by both the cELISA and the VNT until termination of the study. When applied to field samples from Africa, morbillivirus cELISA showed good agreement with a RP cELISA kit (kappa value of 0.86) in bovine sera and with a peste des petits ruminant cELISA kit (kappa value of 0.81) in caprine/ovine sera. Usefulness of morbillivirus cELISA using the rRPV N protein was discussed.     

An anti-plane crack perpendicular to the weak/micro-discontinuous interface in a bi-FGM structure with exponential and linear non-homogeneities

Treated was an anti-plane crack perpendicular to the interface of an exponential-type FGM strip bonded to another linear-type FGM substrate with infinite thickness. Through Fourier integral transform, the problem was reduced as a Cauchy singular integral equation, which was further solved numerically by the Lobatto–Chebyshev collocation method. Based on the numerical solution, the effects of the geometrical and physical parameters on the stress intensity factor (SIF) were analyzed and the following conclusions were obtained: (a) A notable discrepancy between the interface-perpendicular crack and the interfacial one is that, to reduce the weak-discontinuity of interface or to make the interface micro-discontinuous will not necessarily decrease the SIF of the former, but will surely decrease that of the latter. (b) When a crack tip is situated very near to the interface (or free surface), its SIF will be high and totally dominated by the interface (or free surface). (c) To increase the stiffness of the FGM on one side of the interface is beneficial to preventing the crack on the other side from growing toward the interface. Besides, some practical suggestions were further given for material design in the field of composites.     

我国跳汰选煤市场简析

通过对我国跳汰选煤的用户及制造厂家情况的调研，认为跳汰选煤仍是我国主要的选煤工艺。介绍了国内外跳汰机制造厂家的产品经营现状，分析了现存的问题。     

540kN力基准机砝码摆动的研究

日本国家计量研究院(National Research Laboratory of Metrology)540kN静重式力基准机中的振荡分量主要是由于砝码的摆动引起的。本文对砝码的动态特性做了详细的分析，给出了其摆动的轨迹图，并对由于砝码摆动引起的相对不确定度进行了评估。提出了一类采用传感器并联的叠加系统(以下称叠加系统)输出的力值信号来评估砝码动态特性的信号处理方法。它对研究静重式测力机砝码摆动提供了有效的分析方法。     

依靠科技,强基固本,建设"安全发展年"——访山西焦煤集团总经理柴久茂

在2007年夏天一个难得凉爽的日子，我采访了思维严谨、幽默风趣的山西焦煤集团总经理柴久茂。听他解读的企业安全观，既科学实在，又引人深思，很受启发。如果每一个煤炭企业的带头人都有如此理性的安全发展观，那么我国煤矿的真正安宁和谐就会加快实现。     

对油田企业建设和执行内部控制体系的思考

内部控制是一个由企业董事会、管理层和其他员工实施的,为实现企业经营的效果和效率目标、财务报告的可靠性目标和舍规性目标提供合理保证的过程。有效地建设以及有力地执行内控制度,对石油企业发展过程中有效地避免风险的发生起着非常重要的作用。但是,由于种种原因,油田企业目前的内部控制体系还不成熟,还存在着一些建设上的不足和执行上的不力。同时,企业员工普遍对内部控制还存在着一些认识误区,这就要求我们必须努力学习内控知识,加强内控知识培训,充分调动企业全体员工建设和雏护内部控制体系的积极性和自觉性,使得内控可以真正发挥其防范风险的作用。     

新型引线热浸镀锡合金的研究

分析比较了进口锡与国产锡的成分和性能。设计出了新型的引线热浸镀锡合金，它具有较低的工作温度、优良的抗氧化性、流动性和可焊性。     

Superconductivity in Lithium- and Sodium-Doped Mercury-Based Cuprates

The effect of Na- and Li-doping in Hg-based cuprates has been studied. It has been found that superconductivity can be achieved in samples containing small amount of Na or Li. While little change is found in the crystalline structure, the formed superconducting phase have the onset T_c typically above 140 K and zero-resistance T_c up to 136K. More interestingly, the processing temperature for the doped Hg-1223 can be reduced significantly to near 700 °C which is promising for thin films growth on many technologically compatible substrates.     

Statistical properties of out-of-frame detection schemes fordigital transmission systems

The importance of the misframe times for DS1 frame synchronization has often been addressed in designing and using network elements and mediation units. By extending the work of J. H. Eu and W. W. Rollins (1991), Markov chain first passage time analysis is used to derive higher moments of the misframe times in both random and burst error environments. This methodology is of great value in evaluating frame synchronization techniques for digital transmission systems. By experimenting with several out-of-frame detection schemes and reasonable values for bit error ratio (BER), it is shown that misframe times are always approximately exponentially distributed for all schemes