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31.
Katja M?enp?? Ville Ell? Jari Mauno Minna Kellom?ki Riitta Suuronen Timo Ylikomi Susanna Miettinen 《Journal of the Royal Society Interface》2010,7(42):177-188
There is currently no suitable replacement for damaged temporomandibular joint (TMJ) discs after discectomy. In the present study, we fabricated bilayer biodegradable polylactide (PLA) discs comprising a non-woven mat of poly(L/D)lactide (P(L/D)LA) 96/4 and a P(L/DL)LA 70/30 membrane plate. The PLA disc was examined in combination with adipose stem cells (ASCs) for tissue engineering of the fibrocartilaginous TMJ disc in vitro. ASCs were cultured in parallel in control and chondrogenic medium for a maximum of six weeks. Relative expression of the genes, aggrecan, type I collagen and type II collagen present in the TMJ disc extracellular matrix increased in the ASC-seeded PLA discs in the chondrogenic medium. The hypertrophic marker, type X collagen, was moderately induced. Alcian blue staining showed accumulation of sulphated glycosaminoglycans. ASC differentiation in the PLA discs was close to that observed in pellet cultures. Comparison of the mRNA levels revealed that the degree of ASC differentiation was lower than that in TMJ disc-derived cells and tissue. The pellet format supported the phenotype of the TMJ disc-derived cells under chondrogenic conditions and also enhanced their hyalinization potential, which is considered part of the TMJ disc degeneration process. Accordingly, the combination of ASCs and PLA discs has potential for the development of a tissue-engineered TMJ disc replacement. 相似文献
32.
Andrea Kaiser Katja I. Hartmann Dietmar R. Kammerer Reinhold Carle 《European Food Research and Technology》2013,237(3):353-365
Initial heating was applied as the first processing step in the production of innovative pasty celeriac products. For this purpose, celeriac was converted into a paste and subsequently heated at 90 and 100 °C for 5–10 min. Alternatively, the fresh plant material was blanched prior to mincing. For the first time, phenolic compounds in celeriac were characterized by high-performance liquid chromatography coupled with mass spectrometry (LC–MSn). Among the 14 phenolics detected, several caffeic, ferulic and quinic acid derivatives as well as malonylated and acetylated flavonoid derivatives were newly identified. Upon thermal treatment, the antioxidant capacities (TEAC assay) and the total phenolic contents remained virtually unchanged. The antioxidant capacities of heated samples determined by the FRAP assay were even higher than those of the unheated control. The contents of the main phenolic compound apiin decreased upon heat treatment, whereas the levels of the minor compounds malonylapiin A and B increased. Only by extended steam- and water-blanching at 100 °C, respectively, complete inactivation of peroxidase and polyphenol oxidase was achieved. The obtained celeriac products were characterized by their bright white color. Consequently, blanching is recommended as the initial operation in the processing of celeriac into novel pasty products. 相似文献
33.
34.
Public key cryptography has been invented to overcome some key management problems in open networks. Although nearly all aspects of public key cryptography rely on the existence of trapdoor one-way functions, only a very few candidates for this primitive have been observed yet. In this paper, we introduce a new trapdoor one-way permutation based on the hardness of factoring integers of p2q-type. We point out that there are some similarities between Rabin's trapdoor permutation and our proposal. Although our function is less efficient, it possesses a nice feature which is not known for modular squaring, namely there is a variant with a different and easy-to-handle domain. Thus it provides some advantages for practical applications. To confirm this statement, we develop a simple hybrid encryption scheme based on our proposed trapdoor permutation that is CCA-secure in the random oracle model. 相似文献
35.
36.
A study was conducted to evaluate the skill to predict the development of traffic situations. A stop-controlled intersection was filmed over several days, and 12 scenes with varying traffic complexity were selected. In half of the scenes, the traffic rules were violated, in half of the scenes, the rules were observed. A total of 36 participants were asked to watch the scenes and predict how the scene would most likely develop in the 2s after the film was paused. Additionally, the participants rated how certain they were about their prediction, and how complex and dangerous they assessed the scenes to be. With the method used here, experienced drivers were not found to make more correct predictions of situational development, and no difference in skill to predict could be found between genders. Nevertheless, more experienced drivers were more certain in their judgements and evaluated the situations on average as less complex and dangerous than did less experienced drivers. Scenes in which the traffic rules were violated were more difficult to predict correctly. The scenes in which the participants predicted violations were rated as more complex and dangerous. It is concluded that the low-cost method used here is more useful for examining which scenes are generally easy or difficult to predict and how they are experienced subjectively than to investigate differences in performance for different driver categories. 相似文献
37.
Vogel K 《Accident; analysis and prevention》2003,35(3):427-433
The two safety indicators "headway" and "time to collision (TTC)" are discussed and compared with respect to their usefulness in determining the safety of different traffic situations, like different locations in a junction. Over a 6-day-period traffic flow measures were taken in a four-way junction with stop signs on the minor road. It was found that for vehicles in a car following situation headway and TTC are independent of each other. The percentage of small headways is relatively constant across different locations in the junction, while the percentage of small TTC values varies between different locations. It is recommended to use headway for enforcement purposes, because small headways generate potentially dangerous situations. TTC, on the other hand, should be used when a certain traffic environment is to be evaluated in terms of safety, because it indicates the actual occurrences of dangerous situations. 相似文献
38.
A multi-criteria assessment tool for screening preliminary product platform concepts 总被引:2,自引:0,他引:2
Platform concept evaluation is a more challenging task than evaluating a single product concept since a platform must effectively
support multiple product variants over a prolonged period of time. Existing platform methods develop specific criteria in
depth, yet an evaluation of alternative platforms should be based on a broad set of criteria. Based on expert interviews,
personal experience, and a literature search we propose a platform assessment tool consisting of 19 criteria for platform
evaluation. The criteria are group into six categories: customer satisfaction, variety, after-sale, organization, flexibility,
and complexity. The tool is focused on the early platform architecture phase, before proof-of-concept prototyping. However,
it can also be used subsequently for platform refinement when more data becomes available. We demonstrate our platform assessment
tool through an example with a cordless drill platform. 相似文献
39.
The purpose of this paper is to present inverse optimal control as a promising approach to transfer biological motions to
robots. Inverse optimal control helps (a) to understand and identify the underlying optimality criteria of biological motions
based on measurements, and (b) to establish optimal control models that can be used to control robot motion. The aim of inverse
optimal control problems is to determine—for a given dynamic process and an observed solution—the optimization criterion that
has produced the solution. Inverse optimal control problems are difficult from a mathematical point of view, since they require
to solve a parameter identification problem inside an optimal control problem. We propose a pragmatic new bilevel approach
to solve inverse optimal control problems which rests on two pillars: an efficient direct multiple shooting technique to handle
optimal control problems, and a state-of-the art derivative free trust region optimization technique to guarantee a match
between optimal control problem solution and measurements. In this paper, we apply inverse optimal control to establish a
model of human overall locomotion path generation to given target positions and orientations, based on newly collected motion
capture data. It is shown how the optimal control model can be implemented on the humanoid robot HRP-2 and thus enable it
to autonomously generate natural locomotion paths. 相似文献
40.