On optimal parameter selection

Pontryagin's results on optimal parameter selection problems [1, p. 193] have been found to contain some errors, as reported in Boltyanskii [2, p. 263] and Hofer and Sagirow [4]. In this note, it is shown that Boltyanskii's results (on optimal parameters) are easily derived from Gamkrelidze's general maximum principle [3], which, in contrast to the former, also yields an explicit solution to the problem in certain special cases.     

The dependence of the creep properties of din 1.4970 austenitic stainless steel at 973 K on different thermomechanical pre-treatments

The creep-rupture behaviour of a Type DIN 1.4970 austenitic stainless steel has been investigated at 973 K (700°C) in a high vacuum for three conditions of thermomechanical treatment and a wide range of applied stresses using foil specimens of 105 μm thickness. After solution-annealing at 1373 K (1100°C) for all specimens, the three treatments were: — condition 1: 13% cold-worked — condition2: aged for 24 h at 1073 K (800°C) and 13% cold-worked — condition 3: (“Standard condition”): 13% cold-worked and aged for 24 h at 1073 K (800°C).The rupture lives and the minimum creep rates were found to be highly dependent on the applied stresses. The treatment of condition 1 material yielded a product as strong as the “standard” condition 3, while the condition 2 material was less creep resistant.Structural changes as well as fractography were followed using metallographic, transmission and scanning electron microscope techniques. Transgranular ductile fracture was clearly observed in all three conditions. TEM investigations showed that dispersive TiC precipitates were present in the matrix of condition 3 material before creep testing, contrary to condition 1 and 2 material. In condition 1, the TiC dispersion was already found after short creep times, while no dispersive TiC precipitates were found in condition 2 material in any test condition.     

Evaluation and treatment of urinary tract infections in children

Urinary tract infections (UTIs) are among the most common bacterial infections encountered by primary care physicians. Although UTIs do not occur with as great a frequency in children as in adults, they can be a source of significant morbidity in children. For reasons that are not yet completely understood, a minority of UTIs in children progress to renal scarring, hypertension and renal insufficiency. Clinical presentation of UTI in children may be nonspecific, and the appropriateness of certain diagnostic tests remains controversial. The diagnostic work-up should be tailored to uncover functional and structural abnormalities such as dysfunctional voiding, vesicoureteral reflux and obstructive uropathy. A more aggressive work-up, including renal cortical scintigraphy, ultrasound and voiding cystourethrography, is recommended for patients at greater risk for pyelonephritis and renal scarring, including infants less than one year of age and all children who have systemic signs of infection concomitant with a UTI. Antibiotic prophylaxis is used in patients with reflux or recurrent UTI who are at greater risk for subsequent infections and complications.     

Linking assembly code to PASCAL programs for microprocessors

High level languages used for programming microprocessors still need some assembly code to cover all the programming requirements and aspects. Using assembly code in a high level language program introduces certain difficulties and disadvantages. Practical suggestions to minimise the use of assembly code procedures in a high level language program is presented. (The high level language discussed is pascal/64000.)     

Vectors for Cu2+-inducible production of glutathione S-transferase-fusion proteins for single-step purification from yeast

We describe six new yeast episomal vectors which encode glutathione S-transferase (GST) affinity tags. These allow for the production of GST-fusion proteins in Saccharomyces cerevisiae under the control of the CUP1 promoter. Affinity chromatography with glutathione-Sepharose permits convenient purification of the fusion protein from a yeast lysate. The presence of a protease cleavage site facilitates subsequent removal of the GST tag. The expression and single-step purification of both GST and a functional GST-metallothionein fusion from yeast are shown as an example of the application of these vectors.     

Purification and identification of novel antioxidant peptides from enzymatic hydrolysates of sardinelle (Sardinellaaurita) by-products proteins

In order to utilise sardinelle (Sardinellaaurita) protein by-products, which is normally discarded as industrial waste in the process of fish manufacturing, heads and viscera proteins were hydrolysed by different proteases to obtain antioxidative peptides. All hydrolysates showed different degrees of hydrolysis and varying degrees of antioxidant activities. Hydrolysate generated with crude enzyme extract from sardine (Sardinapilchardus) displayed high antioxidant activity, and the higher DPPH radical-scavenging activity (87 ± 2.1% at 2 mg/ml) was obtained with a degree of hydrolysis of 6%. This hydrolysate was fractionated by size exclusion chromatography on a Sephadex G-25 into eight major fractions (P₁–P₈). Fraction P₄, which exhibited the highest DPPH scavenging activity, was then fractionated by reversed-phase high performance liquid chromatography (RP-HPLC). Seven antioxidant peptides were isolated. The molecular masses and amino acids sequences of the purified peptides were determined using ESI-MS and ESI-MS/MS, respectively. Their structures were identified as Leu-His-Tyr, Leu-Ala-Arg-Leu, Gly-Gly-Glu, Gly-Ala-His, Gly-Ala-Trp-Ala, Pro-His-Tyr-Leu and Gly-Ala-Leu-Ala-Ala-His. The first peptide displayed the highest DPPH radical-scavenging activity (63 ± 1.57%; at 150 μg/ml) among these peptides.     

Chemical composition, antioxidant and antibacterial properties of the essential oils of Etlingera elatior and Cinnamomum pubescens Kochummen

BACKGROUND: Plant essential oils are widely used as fragrances and flavours. Therefore, the essential oils from the leaves of Cinnamomum pubescens Kochummen (CP) and the whole plant of Etlingera elatior (EE) were investigated for their antioxidant, antibacterial and phytochemical properties. RESULTS: CP and EE were found to contain appreciable levels of total phenolic contents (50.6 and 33.41 g kg^?1 as gallic acid equivalent) and total flavonoid contents (205.6 and 244.8 g kg^?1 as rutin equivalent), respectively. DPPH free radical scavenging activity of CP is superior to EE (P < 0.05) showing IC₅₀ of 77.2 and 995.1 µg mL^?1, respectively. Methicillin‐resistant Staphylococcus aureus (MRSA), Bacillus subtilis, Pseudomonas aeruginosa and Salmonella choleraesuis were tested against CP and EE. Only MRSA was the most susceptible bacteria to CP. GC/MS studies resulted in the identification of 79 and 73 compounds in CP and EE, respectively. The most abundant components of EE included β‐pinene (24.92%) and 1‐dodecene (24.31%). While the major compound in CP were 1,6‐octadien‐3‐ol,3,7‐dimethyl (11.55%), cinnamaldehyde (56.15%) and 1‐phenyl‐propane‐2,2‐diol diethanoate (11.38%). CONCLUSION: This study suggests that the essential oils from Cinnamomum pubescens Kochummen and Etlingera elatior could be potentially used as a new source of natural antioxidant and antibacterial in the food and pharmaceutical industries. Copyright © 2010 Society of Chemical Industry     

Static and low order anti-windup synthesis for cascade control systems with actuator saturation: An application to temperature-based process control

In this paper, a new framework for designing static and low order anti-windup compensator (AWC) for industrial cascade control systems with actuator saturation constraint is presented. Based on less conservative block diagonal quadratic Lyapunov function, sector boundedness, decoupled architecture, norm reduction and cascade loop compensation, linear matrix inequalities are developed which guarantee stability and suitable performance for overall closed-loop system. Static AWC parameters are obtained by comparing the full order AWC architecture with generalized architecture for cascade control system. Low order AWC is designed by sub-optimal approach in which AWC weights are tuned by designer. Anti-windup compensator is divided into inner and outer loop compensators which compensate the effect of saturation at each level. It is observed that the proposed methodology is less conservative than the traditional AWC schemes when applied to cascade control systems. The proposed scheme is successfully tested experimentally on a temperature-based process control system and results are outlined.