Treatment of Periodontal Inflammation in Diabetic Rats with IL-1ra Thermosensitive Hydrogel

Periodontitis is a chronic inflammatory disease that is considered to be the main cause of adult tooth loss. Diabetes mellitus (DM) has a bidirectional relationship with periodontitis. Interleukin-1β (IL-1β) is an important pre-inflammatory factor, which participates in the pathophysiological process of periodontitis and diabetes. The interleukin-1 receptor antagonist (IL-1ra) is a natural inhibitor of IL-1, and the balance between IL-1ra and IL-1β is one of the main factors affecting chronic periodontitis (CP) and diabetes. The purpose of this study is to develop a drug carrier that is safe and nontoxic and can effectively release IL-1ra, which can effectively slow down the inflammation of periodontal tissues with diabetes, and explore the possibility of lowering the blood sugar of this drug carrier. Therefore, in this experiment, a temperature-sensitive hydrogel loaded with IL-1ra was prepared and characterized, and its anti-inflammatory effect in high-sugar environments in vivo and in vitro was evaluated. The results showed that the hydrogel could gel after 5 min at 37 °C, the pore size was 5–70 μm, and the cumulative release of IL-1ra reached 83.23% on the 21st day. Real-time polymerase chain reaction (qRT-PCR) showed that the expression of IL-1β, Interleukin 6 (IL-6), and tumor necrosis factor α (TNF-α) inflammatory factors decreased after the treatment with IL-1ra-loaded thermosensitive hydrogel. Histological evaluation and micro-computed tomography (Micro-CT) showed that IL-1ra-loaded thermosensitive hydrogel could effectively inhibit periodontal inflammation and reduce alveolar bone absorption in rats with diabetic periodontitis. It is worth mentioning that this hydrogel also plays a role in relieving hyperglycemia. Therefore, the temperature-sensitive hydrogel loaded with IL-1ra may be an effective method to treat periodontitis with diabetes.     

Cyt-C Mediated Mitochondrial Pathway Plays an Important Role in Oocyte Apoptosis in Ricefield Eel (Monopterus albus)

Apoptosis plays a key role in the effective removal of excessive and defective germ cells, which is essential for sequential hermaphroditism and sex change in vertebrates. The ricefield eel, Monopterus albus is a protogynous hermaphroditic fish that undergoes a sequential sex change from female to male. Previous studies have demonstrated that apoptosis is involved in sex change in M. albus. However, the apoptotic signaling pathway is unclear. In the current study, we explored the underlying mechanism of apoptosis during gonadal development and focused on the role of the mitochondrial apoptosis signaling pathway in sex change in M. albus. Flow cytometry was performed to detect apoptosis in gonads at five sexual stages and ovary tissues exposed to hydrogen peroxide (H₂O₂) in vitro. Then the expression patterns of key genes and proteins in the mitochondrial pathway, death receptor pathway and endoplasmic reticulum (ER) pathway were examined. The results showed that the apoptosis rate was significantly increased in the early intersexual stage and then decreased with the natural sex change from female to male. Quantitative real-time PCR revealed that bax, tnfr1, and calpain were mainly expressed in the five stages. ELISA demonstrated that the relative content of cytochrome-c (cyt-c) in the mitochondrial pathway was significantly higher than that of caspase8 and caspase12, with a peak in the early intersexual stage, while the levels of caspase8 and caspase12 peaked in the late intersexual stage. Interestingly, the Pearson’s coefficient between cyt-c and the apoptosis rate was 0.705, which suggests that these factors are closely related during the gonadal development of M. albus. Furthermore, the cyt-c signal was found to be increased in the intersexual stage by immunohistochemistry. After incubation with H₂O₂, the mRNA expression of mitochondrial pathway molecules such as bax, apaf-1, and caspase3 increased in ovary tissues. In conclusion, the present results suggest that the mitochondrial apoptotic pathway may play a more important role than the other apoptotic pathways in sex change in M. albus.     

浅谈教育建筑综合体-以广州中匮药大学国际楼为例

当前校园规划出现了复杂性导向,在校园规划的发展过程中,建筑单体也出现了混合功能的倾向.该文就此倾向进行剖析,并结合广州中医药大学国际楼实际工程提出教育建筑综合体的设计特点.     

基于深度学习的真实尺度运动恢复结构方法

传统的多视图几何方法获取场景结构存在两个问题：一是因图片模糊和低纹理带来的特征点误匹配,从而导致重建精度降低;二是单目相机缺少尺度信息,重建结果只能确定未知的比例因子,无法获取准确的场景结构。针对这些问题本文提出一种基于深度学习的真实尺度运动恢复结构方法。首先使用卷积神经网络获取图片的深度信息;接着为了恢复单目相机的尺度信息,引入惯性传感单元(IMU),将IMU获取的加速度和角速度与ORB-SLAM2获取的相机位姿进行时域和频域上的协同,在频域中获取单目相机的尺度信息;最后将图片的深度图和具有尺度因子的相机位姿进行融合,重建出场景的三维结构。实验表明,使用Depth CNN网络获取的单目图像深度图解决了多层卷积池化操作输出图像分辨率低和缺少重要特征信息的问题,绝对值误差达到了0.192,准确率高达0.959;采用多传感器融合的方法,在频域上获取单目相机的尺度能够达到0.24 m的尺度误差,相比于VIORB方法获取的相机尺度精度更高;重建的三维模型与真实大小具有0.2 m左右的误差,验证了本文方法的有效性。     

螺杆真空泵单头型线变双头方法研究

针对目前发展较为成熟的单头转子型线动不平衡问题,提出了一种将单头转子型线变为双头对称型线的方法。总结了该方法所适用的单头转子型线所需满足的条件,并用数学方法证明了所得到的双头型线能够正确啮合。定性分析计算了型线从单头变为双头后面积的变化,并以特定单头型线为例,计算了型线面积利用系数的变化。将特定单头型线变换得到对应的双头对称型线,用Solidworks三维软件建立转子模型并进行两个转子的装配,模拟验证了双头型线的正确性。该方法保留了原型线的接触线、泄漏三角形等特性,得到了具有较好动平衡性的双头型线。     

ICP-MS法测定白酒中Mn和毒理性元素的研究

建立电感耦合等离子体质谱（ICP-MS）法测定白酒中Mn和9个毒理性元素含量的分析方法。白酒采用硝酸酸化并加入低浓度盐酸后测定了其中的Be、Cr、Mn、Ni、As、Cd、Sn、Sb、Hg、Pb等元素,盐酸的加入有效地改善了样品溶液中元素的长时间稳定性,尤其是元素Hg和Sn。应用八极杆碰撞/反应池（ORS）技术有效地消除了盐酸的使用所带入的氯离子干扰以及其他质谱干扰,采用基体匹配并通过加入内标溶液有效地补偿样品传输的影响以及溶剂蒸发速率的差异,消除了基体所产生的影响。在选定的工作条件下,方法的线性关系良好,各元素的检出限为0.13¹2.15 ng/L之间,相对标准偏差（RSD）在0.75%³.48%之间,样品的加标回收率为93.80%¹10.00%。该方法具有操作简单、快速、结果准确等特点,可用于白酒的质量控制和安全评价。      

温度对调和油品质的影响

以水酶法调和油、溶剂浸提调和油、市售调和油为研究对象,探讨在100、150、200、250、300℃条件下加热1h,各调和油理化指标的变化。结果表明:随着加热温度的升高,3种调和油的色泽加深,酸价、p-茴香胺值明显增加,过氧化值均先上升后下降,三种调和油的饱和脂肪酸含量增加,不饱和脂肪酸含量降低,但在加热温度低于200℃时,水酶法调和油的脂肪酸比例仍能接近中国营养学会推荐的均衡比例0.27∶1∶1。      

纳米等离子体激光器研究进展

半导体激光器在生物技术、信息存储、光子医学诊疗等方面得到了广泛应用。随着纳米技术和纳米光子学的发展,紧凑微型化激光器应用前景引人关注。当激光器谐振腔尺寸减小到发射波长时,电磁谐振腔中将产生更为有趣的物理效应。因此,在发展低维、低泵浦阈值的超快相干光源,以及纳米光电集成和等离激元光路时,减小半导体激光器的三维尺寸至关重要。在本综述中,首先介绍了纳米等离子体激光器中的谐振腔模式增益和限制因子的总体理论,并综述了金属-绝缘材料-半导体纳米(MIS)结构或其它相关金属覆盖半导体结构的纳米等离子体激光器各方面的总体研究进展。特别地,对基于MIS结构的等离子体谐振腔实现纳米等离子体激光器三维衍射极限的突破,进行了详细的介绍。本文也介绍并展望了纳米等离子体激光器的技术挑战和发展趋势,为纳米激光器进一步研究提供参考。