Encephalocytozoon intestinalis-specific monoclonal antibodies for laboratory diagnosis of microsporidiosis

Two monoclonal antibodies which can be used for the unambiguous identification by fluorescence microscopy of Encephalitozoon intestinalis spores in clinical specimens are described. Monoclonal antibody Si91 is specific for the extruded polar filament, and Si13 recognizes the surfaces of E. intestinalis spores. No cross-reaction with spores of Encephalitozoon hellem was observed. Immunogold electron microscopy confirmed the specific reactivities of both antibodies. Combined in an indirect immunofluorescence assay, these antibodies are used to identify spores in feces. Although there was some cross-reaction with fecal bacteria and fungi, the typical morphology of the extruded polar filaments enabled proper identification of the E. intestinalis spores. Parasites could also be demonstrated to be present in urine, nasal swabs, lung brush biopsy specimens, and bronchoalveolar lavage fluid from a patient with disseminated infection with E. intestinalis. The use of these monoclonal antibodies facilitates the detection and species determination of E. intestinalis in clinical specimens.     

Performance of fractionating reactors in the absence of rate limitations

A fractionating reactor for equilibrium‐limited reactions is studied theoretically. Reactant A is fed in the center of the countercurrent fractionating system. Product P is effectively transported with the auxiliary phase, while product Q is effectively transported with the main phase, in which the reaction takes place. Model calculations were based on partition and reaction equilibrium at all stages. These show that if the initial reactant concentration and the flow rates are properly selected, the extent of conversion will significantly exceed the corresponding batch conversion. To approach complete conversion in the fractionating reactor, and to recover both products in a pure form, net transport of reactant in either of the countercurrent directions should be prevented. However, irrespective of the number of equilibrium stages, this situation cannot be fully reached when the reactant feed stream is too large (compared with the main and auxiliary streams). Nonetheless, one of the two products may be recovered in a pure form even for such large feed streams. Copyright © 2004 Society of Chemical Industry     

Migration and foreign direct investment: education matters

The rapid growth in the foreign-born population in many high- and middle-income countries in recent decades has prompted much research on the socio-economic determinants and impacts of immigration. This paper investigates the relationship between the stock of foreign population by nationality living in the UK and the bilateral stock of foreign direct investment (FDI), both inward FDI into the UK and outward FDI from the UK. This study contributes to the literature on the above-mentioned association between migration and FDI, by using the UK annual data from 2001 to 2007 for 22 countries on the inward volume of FDI and for 27 countries on the outward volume of FDI. Our study finds a significant and positive relationship between migration and outward FDI. This result also holds, if we correct for endogeneity by using an instrumental variable approach. If we then include the education level of migrants living in the UK, our results indicate that the more educated migrants from a certain country are, the stronger positive effect they have on FDI in both directions (inward and outward).     

Reactive Spray Deposition Technology - An one-step deposition technique for Solid Oxide Fuel Cell barrier layers

In order to reduce the cost of the manufacturing of Solid Oxide Fuel Cells (SOFC), and to enable metal supported cell fabrication, a new fabrication method called Reactive Spray Deposition Technology (RSDT) for direct deposition of the material onto ceramic or metal support for low temperature SOFC is currently being developed. The present work describes the effect on the performance of a SOFC when a Gd_0.2Ce_0.8O_1.9 (GDC) layer has been introduced as diffusion barrier layer between the yttria stabilized zirconia (YSZ) electrolyte and the La_0.6Sr_0.4CoO_3−δ (LSC) cathode. The dense, thin and fully crystalline GDC films were directly applied by RSDT, without any post-deposition heating or sintering step. The quality of the film and performance of the cell prepared by RSDT was compared to a GDC blocking layer deposited by screen printing (SP) and then sintered. The observed ohmic resistance of the ASC with a GDC layer deposited by RSDT is 0.24 Ω cm², which is close to the expected theoretical value of 0.17 Ω cm² for a 5-μm thick 8 mol% yttria YSZ (8YSZ) electrolyte at 873 K.     

Dynamic MRI of swallowing: real-time volumetric imaging at 12 frames per second at 3 T

Objective

Dysphagia or difficulty in swallowing is a potentially hazardous clinical problem that needs regular monitoring. Real-time 2D MRI of swallowing is a promising radiation-free alternative to the current clinical standard: videofluoroscopy. However, aspiration may be missed if it occurs outside this single imaged slice. We therefore aimed to image swallowing in 3D real time at 12 frames per second (fps).

Materials and methods

At 3 T, three 3D real-time MRI acquisition approaches were compared to the 2D acquisition: an aligned stack-of-stars (SOS), and a rotated SOS with a golden-angle increment and with a tiny golden-angle increment. The optimal 3D acquisition was determined by computer simulations and phantom scans. Subsequently, five healthy volunteers were scanned and swallowing parameters were measured.

Results

Although the rotated SOS approaches resulted in better image quality in simulations, in practice, the aligned SOS performed best due to the limited number of slices. The four swallowing phases could be distinguished in 3D real-time MRI, even though the spatial blurring was stronger than in 2D. The swallowing parameters were similar between 2 and 3D.

Conclusion

At a spatial resolution of 2-by-2-by-6 mm with seven slices, swallowing can be imaged in 3D real time at a frame rate of 12 fps.

     

Good modelling practice in drinking water treatment,applied to Weesperkarspel plant of Waternet

Good modelling practice increases the credibility and impact of the information and insight that modelling aims to generate. It is known to be crucial for model acceptance and it is a necessity to amass a long-term, systematic thorough knowledge base for both science and decision making. This paper shows how ten steps in model development and evaluation can also be applied to numerical modelling of drinking water treatment, using models of drinking water treatment processes of the Weesperkarspel treatment plant of Waternet. The Weesperkarspel plant consists of ozonation, pellet softening, biological activated carbon filtration and slow sand filtration. For the different processes models were developed that were used for operational improvements. The modelling resulted in new insights and knowledge about the treatment processes and improved operation of the processes. From scenario studies for the pellet softening it was concluded that chemical dosing can be diminished when by-pass ratio is increased and that pellet size can be controlled by measuring the difference in pressure guaranteeing fluidisation of the pellet bed. In addition, ozone dosage can be optimised by modelling ozone exposure, bromate formation and biologically degradable natural organic matter (NOM) under varying influent water quality.     

Model-based operational constraints for fluidised bed crystallisation

Fluidised bed reactors are used for water softening in water treatment plants. Recent research shows that under current operation of reactors the crystallisation of calcium carbonate can be hampered. Until now the operational constraints on the fluidised bed have not been exactly known. Experiments were carried out to investigate the fluidisation behaviour of calcium carbonate pellets in water. The results of the fluidisation experiments are compared to two commonly used modelling approaches of Ergun and Richardon-Zaki. Using the experimental data the models are calibrated. The calibrated Richardson-Zaki model is used to determine operational constraints on pellet size at the bottom of the reactor and water flow through the reactor. The model-based constraints are compared to operational data of the Weesperkarspel full-scale treatment plant of Waternet (The Netherlands). It can be concluded that the current operation of the treatment plant violates the calculated constraints with consequences for effluent quality and corrective maintenance. By using models for determining the operation of the fluidised bed, the softening process can thus be improved.     

Ex Ante Impact Assessment of Reservoir Construction Projects for Different Stakeholders Using Agent-Based Modeling

Reservoir construction projects are frequently met with fierce opposition. Consequently, to mitigate any potential negative impacts, environmental and social impact assessments are usually mandatory. Stakeholder perspectives are often only implicit in such assessments, and the medium-term effects of mitigating actions are assessed at the aggregate level, which fails to take into account unequal disaggregated impacts. In this paper, we design and apply an agent-based model (ABM) built on stakeholder information to conduct an ex ante assessment of the impact of a reservoir construction project in southern Thailand over a 30-year period for individual agents. We incorporate stakeholders’ knowledge into the ABM on the basis of primary data collected during the 2016–2018 period, including workshops with affected farmers to assess their interests and concerns, in-depth interviews with farmers in nearby districts to assess farming behaviors, and the expert opinions of policymakers to assess the relevant regulations and processes. In a case study for which the model was established, the results predict that, overall, farmers will have more farm income if the dam is built. We find that affected people require a standard of living similar to that provided by their previous livelihood as soon as possible after resettlement. By simulating the impacts on individual agents, we conclude that the compensation for relocation offered to affected farmers is not sufficient for sustainable resettlements. Facilitating compensation may increase the speed of project implementation and lead to better outcomes for everyone, including affected communities, whereas failure to reshape the current compensation policy leaves everyone more disadvantaged.

     

Design of Active Interfaces Using Responsive Molecular Components

Responsive interfaces are interfaces that show a defined and reversible change in physical properties in response to external stimuli. Typically, responsive interfaces result from the immobilization of responsive molecular components at the interface that translate a nanoscale signal into a macroscopic effect. Responsive interfaces can also be obtained if the topology of the interface can be reversibly changed using an external stimulus. As the surface of any material is its connection to the environment, responsive interfaces provide opportunities for interactive materials which are not only able to change properties upon demand, but also sense their environment and act autonomously. The application of responsive molecular components at interfaces, however, requires chemical and physical compatibility with the material surface of interest, posing a challenge not least in the retention of the responsive functionality. The state of the art in “active” interfaces which display responsive wettability, permeability, or adhesion is discussed, with a particular emphasis on microscale and nanoscale patterning since patterned interfaces can give rise to unique material properties. Finally, perspectives in the development of responsive interfaces, as well as promising approaches for bypassing the most prominent challenges are discussed.