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21.
MR Workman PG Wall P Tearle M O'Mahony WA Brunton 《Canadian Metallurgical Quarterly》1995,5(4):R54-R56
In microbiology laboratories highly infectious material is handled alongside complex and potentially dangerous equipment, and staff are therefore at risk of infections and accidents. Acts of parliament and regulations exist to protect staff in the workplace, including those exposed to biological agents. The current monitoring of health and safety in laboratories seeks to ensure that employers and employees comply with existing regulations, but this form of passive surveillance is of limited value because it does not highlight shortcomings in techniques, equipment, premises, or personnel. We propose a scheme for the surveillance of health and safety in microbiology laboratories that will actively seek information about laboratory incidents and practices, in order to enable appropriate preventive measures to be instituted. 相似文献
22.
23.
Matthew N. Ashby Patrick R. Errada Victor L. Boyartchuk Jasper Rine 《Yeast (Chichester, England)》1993,9(8):907-913
We isolated a mutant defective in C-terminal farnesyl cysteine:carboxyl methyltransferase activity from a screen for mutations causing a -specific sterility. A genomic fragment was cloned from a yeast multi-copy library that restored mating. Both the cloned gene and the sterile mutation were allelic to the STE14 gene. A ste14-complementing 2·17 kb BamHI fragment subclone was sequenced and found to encode a 239 amino acid protein with a molecular weight of 27,887 Daltons. The hydrophobicity profile of the methyltransferase reveals the presence of at least five potential transmembrane domains. In comparisons of the C-terminal methyltransferase amino acid sequence with those in the PIR and Swiss protein databases, no significantly similar sequences were found nor were conserved regions from other methyltransferases present. 相似文献
24.
25.
M. E. Kassner M. A. Wall M. A. Delos-Reyes 《Metallurgical and Materials Transactions A》1997,28(3):595-609
Aluminum single crystals were cyclically deformed in single slip at small strain amplitudes at 77 K to presaturation. The
observed mechanical behavior is consistent with recent work by other investigators. The dislocation substructure can be described
as consisting of dense bundles or veins of edge dislocation dipoles, of a single Burgers vector, separated by lower dislocation
density regions or channels where substantial debris is evident. This debris was determined as almost exclusively relatively
short edge-dipole segments. Screw dislocations with the same Burgers vector span the channels. In situ cyclic reverse (shear) deformation experiments in the high-voltage transmission electron microscope (HVEM) were successfully
performed using the X-Y technique where thin foils are stressed in alternating perpendicular directions. Our experiments indicate that loops frequently
expand from the dipole bundles into the channel and the edge component is absorbed by nearby bundles, leaving screw segments
behind. The screw dislocations that span the channel move easily and reverse direction with shear reversal. Screws may move
first with a strain reversal. A comparable fraction of the strain during each cycle appears to be provided by screw and edge
dislocations. Dipole “flipping” was not observed. There is no obvious evidence for internal backstresses that assist plastic
deformation on reversal of the applied shear. 相似文献
26.
Examined publishing practices of the American Psychological Association (APA) in the alcohol and drug area. Although the total number of articles published by APA increased only 7.6% between 1984 and 1990, the number of alcohol and drug articles more than doubled. Despite the disproportionate growth of alcohol and drug articles between 1984 and 1990, only 3.8% of 1,408 APA articles published in 1990 addressed alcohol and drug use issues. An earlier investigation by M. O. Howard and D. A. Howard (1992) reported that 541 articles published in 1984 in 12 alcohol and drug specialty journals received an average of 3.48 citations by the end of 1988. Alcohol and drug articles published by APA in 1984 received more than 4 times as many citations during this period. Also, alcohol and drug articles published by APA received significantly more citations between 1984 and 1988 than those published in specialty journals. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
27.
28.
Artificial added mass instabilities in sequential staggered coupling of nonlinear structures and incompressible viscous flows 总被引:1,自引:0,他引:1
Christiane Förster Wolfgang A. Wall 《Computer Methods in Applied Mechanics and Engineering》2007,196(7):1278-1293
Within this paper the so-called artificial added mass effect is investigated which is responsible for devastating instabilities within sequentially staggered Fluid-structure Interaction (FSI) simulations where incompressible fluids are considered.A discrete representation of the added mass operator MA is given and ‘instability conditions’ are evaluated for different temporal discretisation schemes. It is proven that for every sequentially staggered scheme and given spatial discretisation of a problem, a mass ratio between fluid and structural mass density can be found at which the coupled system becomes unstable. The analysis is quite general and does not depend upon the particular spatial discretisation schemes used. However here special attention is given to stabilised finite elements employed on the fluid partition. Numerical investigations further highlight the results. 相似文献
29.
OBJECTIVE: To determine the results peranal excision for rectal carcinoma. DESIGN: Retrospective case series. SETTING: A university-affiliated hospital. PATIENTS: Of 178 patients who presented for curative resection of rectal carcinoma between 1975 and 1993, 19 (10.7%) were deemed suitable for local excision. There were 10 men and 9 women with a mean age of 71.2 years. The follow-up ranged from 13 to 184 months. INTERVENTION: Peranal excision. MAIN OUTCOME MEASURES: Histologic differentiation, gross morphology, depth of invasion and size of the carcinoma, adequacy of margins of excision, complications of operation, rates of recurrence, results of salvage therapy and 5-year survival. RESULTS: There were no intraoperative complications. Postoperative complications included urinary retention (one patient) and bleeding (one patient). There were five local recurrences (26%). Salvage operations were performed in three (60%) patients and were successful in two of them. The 5-year cancer-specific survival rate was 82%. The recurrence rate was higher in patients with inadequate margins of excision and ulcerative lesions. Neither size nor grade of the carcinoma correlated with recurrence. CONCLUSIONS: Local excision of rectal carcinoma can be performed successfully in selected patients. Diligent follow-up is required, because up to 60% of local recurrences can be treated successfully. 相似文献
30.
BA Posner MB Mixon MA Wall SR Sprang AG Gilman 《Canadian Metallurgical Quarterly》1998,273(34):21752-21758
Agonist-bound heptahelical receptors activate heterotrimeric G proteins by catalyzing exchange of GDP for GTP on their alpha subunits. In search of an approximation of the receptor-alpha subunit complex, we have considered the properties of A326S Gialpha1, a mutation discovered originally in Gsalpha (Iiri, T., Herzmark, P., Nakamoto, J. M., Van Dop, C., and Bourne, H. R. (1994) Nature 371, 164-168) that mimics the effect of receptor on nucleotide exchange. The mutation accelerates dissociation of GDP from the alphai1beta1gamma2 heterotrimer by 250-fold. Nevertheless, affinity of mutant Gialpha1 for GTPgammaS is high in the presence of Mg2+, and the mutation has no effect on the intrinsic GTPase activity of the alpha subunit. The mutation also uncouples two activities of betagamma: stabilization of the GDP-bound alpha subunit (which is retained) and retardation of GDP dissociation from the heterotrimer (which is lost). For wild-type and mutant Gialpha1, beta gamma prevents irreversible inactivation of the alpha subunit at 30 degreesC. However, the mutation accelerates irreversible inactivation of alpha at 37 degreesC despite the presence of beta gamma. Structurally, the mutation weakens affinity for GTPgammaS by steric crowding: a 2-fold increase in the number of close contacts between the protein and the purine ring of the nucleotide. By contrast, we observe no differences in structure at the GDP binding site between wild-type heterotrimers and those containing A326S Gialpha1. However, the GDP binding site is only partially occupied in crystals of G protein heterotrimers containing A326S Gialpha1. In contrast to original speculations about the structural correlates of receptor-catalyzed nucleotide exchange, rapid dissociation of GDP can be observed in the absence of substantial structural alteration of a Galpha subunit in the GDP-bound state. 相似文献