Magneto‐Thermal Metrics Can Mirror the Long‐Term Intracellular Fate of Magneto‐Plasmonic Nanohybrids and Reveal the Remarkable Shielding Effect of Gold

Multifunctional nanoparticles such as magneto‐plasmonic nanohybrids are rising theranostic agents. However, little is yet known of their fate within the cellular environment. In order to reach an understanding of their biotransformations, reliable metrics for tracking and quantification of such materials properties during their intracellular journey are needed. In this study, their long‐term (one month) intracellular fate is followed within stem‐cell spheroids used as tissue replicas. A set of magnetic (magnetization) and thermal (magnetic hyperthermia, photothermia) metrics is implemented to provide reliable insightsinto the intracellular status. It shows that biodegradation is modulated by the morphology and thickness of the gold shell. First a massive dissolution of the iron oxide core (nanoflower‐like) is observed, starting with dissociation of the multigrain structure. Second, it is demonstrated that an uninterrupted gold shell can preserve the magnetic core and properties (particularly magnetic hyperthermia). In addition to the magnetic and thermal metrics, intracellular high‐resolution chemical nanocartography evidences the gradual degradation of the magnetic cores. It also shows different transformation scenarios, from the release of small gold seeds when the magnetic core is dissolved (interesting for long‐term elimination) to the protection of the magnetic core (interesting for long‐term therapeutic applicability).     

STEP-NC compliant process planning of additive manufacturing: remanufacturing

Additive manufacturing is becoming one of the key methods for reproducing repair sections in remanufacturing processes. The major advantage of using additive processes is to minimize production time and waste. However, the surface quality and shape accuracy are usually insufficient for the final product because the approximated representation format causes the accumulation of the error during the geometric operations of the process planning. This limitation is a barrier to utilize additive processes as finishing processes, such as general metal cutting. There is need to improve the final quality of parts obtained with additive manufacturing. In this paper, STEP-based numerical control (STEP-NC)-based process planning is applied to the additive manufacturing. ISO 14649 (STEP-NC) describes part programs with geometric data directly and also contains the information necessary for the intelligent process planning. This paper proposes the STEP-NC-based representation method of additive manufacturing and the series of geometric reasoning to automate the derivation of the repair section. The proposed representation has the benefits to provide a high accuracy for the final surface and to describe multiple materials. Topological data maintain low error during the series of process planning through the CAD-CAM-CNC chain. The proposed platform supports consideration of the process tolerance and comparison of the selected plan with alternative processes. In order to show the practical advantages, an analysis of the remanufacturing process is carried out. The case study of remanufacturing a pocket part is presented in order to validate the proposed process plan. The result of the case study shows the improvement in terms of automatic process planning and surface quality accuracy.     

Same Neighbourhood … Different Views? A Confrontation of Internal and External Neighbourhood Reputations

Residents and non-residents are likely to think differently about a neighbourhood's reputation. Relatively little is known about the similarities and differences between these internal and external types of neighbourhood reputation or the relationship between reputations and ‘real’ or ‘objective’ neighbourhood characteristics. This paper addresses two points: first, the extent to which neighbourhood reputations differ between and within groups; second, the extent to which these neighbourhood reputations are associated with measured neighbourhood characteristics. Data from a specially designed survey carried out in 24 neighbourhoods in Utrecht, the fourth largest city in the Netherlands, are used. Analysis of the data showed that neighbourhood reputations are rated higher by residents and estate agents than by other city residents. Within the group of other city residents, differences were found in how neighbourhood reputations are rated by socio-economic status, ethnicity and educational background. Further, it was found that neighbourhood reputations are correlated with measured social characteristics of the neighbourhood, while physical and functional neighbourhood characteristics are of less importance.     

Moisture balance assessment at room scale for four cases based on numerical simulations of heat–air–moisture transfers for a realistic occupancy scenario

The moisture balance at room scale is influenced by the air change rate, moisture production and vapour transfer with the envelope. However, results may differ strongly from one study to another depending on the sizing of the ventilation system, the boundary conditions and the modelling of vapour transfer in the walls. This paper aims to provide a realistic comparison of usual construction techniques based on energy consumption, indoor comfort and durability. To achieve this objective, an existing whole-building heat–air–moisture simulation tool was selected to compute coupled transfer at room scale over an entire year. Moisture production due to occupancy was modelled using a stochastic approach. Four cases were selected to emphasize the differences of both a vapour-permeable wall assembly and a relative humidity sensitive ventilation system compared to common practices.     

3D electron microscopy study of metal particles inside multiwalled carbon nanotubes

The location of palladium nanoparticles on and inside the multiwalled carbon nanotubes channel is presented for the first time using electron tomography (3D TEM). The palladium salt precursor was rapidly sucked inside the nanotube channel by means of capillarity that is favored by the hydrophilic character of the tube wall after acidic treatment at low temperature. Statistical analysis indicates that the palladium particles were well dispersed and the palladium particle size was relatively homogeneous, ranging from 3 to 4 nm regardless of their location within the nanotube, within the resolution limit of the technique for our experimental conditions, i.e., about 2 nm. Three-dimensional TEM analysis also revealed that introduction of foreign elements inside the tube channel is strongly influenced by the diameter of the tube inner channel, i.e., easy filling seems to occur with a tube channel >or=30 nm , whereas with tubes having a smaller channel (<15 nm), almost no filling by capillarity occurred leading to the deposition of the metal particles only on the outer wall of the tube.     

Inhibition of Very Long Chain Fatty Acids Synthesis Mediates PI3P Homeostasis at Endosomal Compartments

A main characteristic of sphingolipids is the presence of a very long chain fatty acid (VLCFA) whose function in cellular processes is not yet fully understood. VLCFAs of sphingolipids are involved in the intracellular traffic to the vacuole and the maturation of early endosomes into late endosomes is one of the major pathways for vacuolar traffic. Additionally, the anionic phospholipid phosphatidylinositol-3-phosphate (PtdIns (3)P or PI3P) is involved in protein sorting and recruitment of small GTPase effectors at late endosomes/multivesicular bodies (MVBs) during vacuolar trafficking. In contrast to animal cells, PI3P mainly localizes to late endosomes in plant cells and to a minor extent to a discrete sub-domain of the plant’s early endosome (EE)/trans-Golgi network (TGN) where the endosomal maturation occurs. However, the mechanisms that control the relative levels of PI3P between TGN and MVBs are unknown. Using metazachlor, an inhibitor of VLCFA synthesis, we found that VLCFAs are involved in the TGN/MVB distribution of PI3P. This effect is independent from either synthesis of PI3P by PI3-kinase or degradation of PI(3,5)P₂ into PI3P by the SUPPRESSOR OF ACTIN1 (SAC1) phosphatase. Using high-resolution live cell imaging microscopy, we detected transient associations between TGNs and MVBs but VLCFAs are not involved in those interactions. Nonetheless, our results suggest that PI3P might be transferable from TGN to MVBs and that VLCFAs act in this process.     

SOCRATE: an optical bench dedicated to the understanding and improvement of a laser conditioning process

We present an automatic excimer laser bench (SOCRATE) allowing for the treatment of optical components by laser conditioning. This apparatus, developed at the Commissariat a l'Energie Atomique-Le Ripault, has been designed to add to this conditioning process an in situ, accurate laser-induced damage threshold (LIDT) measurement and different nondestructive optical techniques for the characterization of the component during treatment. Through different examples, we demonstrate the importance of these characterizations to improve the understanding of the laser conditioning. The role of an in situ adapted metrology associated in real time with a laser conditioning bench offers new opportunities to analyze laser-induced damage mechanisms and subsequently to increase the LIDT of optical components.     

Predictive modeling of piezoelectric wafer active sensors interaction with high-frequency structural waves and vibration

The modeling of the interaction between piezoelectric wafer active sensors (PWAS) and structural waves and vibration is addressed. Three main issues are discussed: (a) modeling of pitch-catch ultrasonic waves between a PWAS transmitter and a PWAS receiver by comparison between exact Lamb wave solutions and various finite element method (FEM) results; (b) analytical modeling of the power and energy transduction between PWAS and ultrasonic guided waves highlighting the tuning opportunities between PWAS and the waves; (c) the use of the transfer matrix method to model the electromechanical (E/M) impedance method for direct reading of high-frequency local structural vibration and comparison with FEM results. The paper ends with a summary and conclusions followed by recommendations for further work.     

Pattern-integrated interference lithography: single-exposure fabrication of photonic-crystal structures

Multibeam interference represents an approach for producing one-, two-, and three-dimensional periodic optical-intensity distributions with submicrometer features and periodicities. Accordingly, interference lithography (IL) has been used in a wide variety of applications, typically requiring additional lithographic steps to modify the periodic interference pattern and create integrated functional elements. In the present work, pattern-integrated interference lithography (PIIL) is introduced. PIIL is the integration of superposed pattern imaging with IL. Then a pattern-integrated interference exposure system (PIIES) is presented that implements PIIL by incorporating a projection imaging capability in a novel three-beam interference configuration. The purpose of this system is to fabricate, in a single-exposure step, a two-dimensional periodic photonic-crystal lattice with nonperiodic functional elements integrated into the periodic pattern. The design of the basic system is presented along with a model that simulates the resulting optical-intensity distribution at the system sample plane where the three beams simultaneously interfere and integrate a superposed image of the projected mask pattern. Appropriate performance metrics are defined in order to quantify the characteristics of the resulting photonic-crystal structure. These intensity and lattice-vector metrics differ markedly from the metrics used to evaluate traditional photolithographic imaging systems. Simulation and experimental results are presented that demonstrate the fabrication of example photonic-crystal structures in a single-exposure step. Example well-defined photonic-crystal structures exhibiting favorable intensity and lattice-vector metrics demonstrate the potential of PIIL for fabricating dense integrated optical circuits.     

Multiscale transparent electrode architecture for efficient light management and carrier collection in solar cells

The challenge for all photovoltaic technologies is to maximize light absorption, to convert photons with minimal losses into electric charges, and to efficiently extract them to the electrical circuit. For thin-film solar cells, all these tasks rely heavily on the transparent front electrode. Here we present a multiscale electrode architecture that allows us to achieve efficiencies as high as 14.1% with a thin-film silicon tandem solar cell employing only 3 μm of silicon. Our approach combines the versatility of nanoimprint lithography, the unusually high carrier mobility of hydrogenated indium oxide (over 100 cm(2)/V/s), and the unequaled light-scattering properties of self-textured zinc oxide. A multiscale texture provides light trapping over a broad wavelength range while ensuring an optimum morphology for the growth of high-quality silicon layers. A conductive bilayer stack guarantees carrier extraction while minimizing parasitic absorption losses. The tunability accessible through such multiscale electrode architecture offers unprecedented possibilities to address the trade-off between cell optical and electrical performance.