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81.
Hosokawa F Tomita T Naruse M Honda T Hartel P Haider M 《Journal of electron microscopy》2003,52(1):3-10
A spherical aberration (Cs)-corrected 200 kV TEM was newly developed. The column of the microscope was extended by 25 cm and the inner yoke of the objective lens was modified to insert some parts of the corrector elements. The corrector has two hexapole elements that play a main role in Cs correction and they are placed at a position equivalent to the coma-free point of the objective lens by using two transfer doublet lenses. The Cs correction was successfully carried out by means of the third-order aberration that was generated in the two extended hexapoles. The Cs can be corrected to the desired value and also can be overcompensated in order to produce a negative Cs, as with the corrected Cs of -23 microm shown in this work. The optical system of the corrector does not produce second- and fourth-order aberrations, and can correct residual aberrations up to the third order. All of the corrector elements are computer-controlled and the third-order aberrations are quite stable after they are properly corrected. The resolution of 0.135 nm was experimentally confirmed by the Young's fringe method. Image simulations of a silicon [110] single crystal were made with various Cs and defocus values to demonstrate the effectiveness of arbitral control of Cs. 相似文献
82.
Nunziante M Gilch S Schätzl HM 《Chembiochem : a European journal of chemical biology》2003,4(12):1268-1284
Prion diseases are fatal neurodegenerative infectious disorders for which no therapeutic or prophylactic regimens exist. Understanding the molecular process of conformational conversion of the cellular prion protein (PrP(c)) into its pathological isoform (PrP(Sc)) will be necessary to devise effective antiprion strategies. In recent years, new findings in the cell biology of PrP(c), in the molecular pathogenesis of PrP(Sc), and in the cellular quality control mechanisms involved in these scenarios have accumulated. A function of the prion protein in signalling, the possible impact of the proteasome, and aggresomes as intracellular waste deposits have been described. Here, important pathogenetic similarities with the more frequent neurodegenerative disorders are evident. The need for therapeutic, postexposure, and prophylactic possibilities was drastically illustrated by the emergence of variant Creutzfeldt-Jakob disease (vCJD), a new human prion disease caused by bovine spongiform encephalopathy (BSE) derived prions. Although prion infectivity in humans is usually restricted to the central nervous system, in vCJD patients prions are present in the lympho-reticular system, posing a theoretical risk of accidental human-to-human transmission. A variety of chemical antiprion substances have been reported in in vitro and cell culture based assays or in animal studies. Occasionally, they have also made their way into the first human trials. In addition, various promising interference strategies have been devised in transgenic models, although they are usually hard to transfer into nontransgenic in vivo situations. New findings in the fields of peripheral prion pathogenesis and immune system involvement fuelled the search for antiprion strategies formerly considered to be entirely impossible. This opened the door towards classical immunological interference techniques. Remarkably, passive and even active vaccination approaches now seem to be realistic goals. 相似文献
83.
Wilson Keith G.; Chochinov Harvey Max; McPherson Christine J.; Skirko Merika Graham; Allard Pierre; Chary Srini; Gagnon Pierre R.; Macmillan Karen; De Luca Marina; O'Shea Fiona; Kuhl David; Fainsinger Robin L.; Karam Andrea M.; Clinch Jennifer J. 《Canadian Metallurgical Quarterly》2007,26(3):314
Objective: To investigate the attitudes of terminally ill individuals toward the legalization of euthanasia or physician-assisted suicide (PAS) and to identify those who would personally desire such a death. Design: In the Canadian National Palliative Care Survey, semistructured interviews were administered to 379 patients who were receiving palliative care for cancer. Patients who expressed a desire for physician-hastened death were followed prospectively. Main Outcome Measures: Attitudes toward the legalization of euthanasia or PAS were determined, as was the personal interest in receiving a hastened death. Demographic and clinical characteristics were also recorded, including a 22-item structured interview of symptoms and concerns. Results: There were 238 participants (62.8%) who believed that euthanasia and/or PAS should be legalized, and 151 (39.8%) who would consider making a future request for a physician-hastened death. However, only 22 (5.8%) reported that, if legally permissible, they would initiate such a request right away, in their current situations. This desire for hastened death was associated with lower religiosity (p = .010), reduced functional status (p = .024), a diagnosis of major depression (p 相似文献
84.
In this paper, the novel inverse synthetic aperture secondary radar wireless positioning technique is introduced. The proposed concept allows for a precise spatial localization of a backscatter transponder even in dense multipath environments. A novel secondary radar signal evaluation concept compensates for the unknown modulation phase of the returned signal and thus leads to radar signals comparable to common primary radar. With use of this concept, inverse synthetic aperture radar algorithms can be applied to the signals of backscatter transponder systems. In simulations and first experiments, we used a broadband holographic reconstruction principle to realize the inverse synthetic aperture approach. The movement of the transponder along a short arbitrary aperture path is determined with assisting relative sensors (dead reckoning or inertia sensors). A set of signals measured along the aperture is adaptively focused to the transponder position. By this focusing technique, multipath reflections can be suppressed impressively and a precise indoor positioning becomes feasible. With our technique, completely new and powerful options for integrated navigation and sensor fusion in RF identification systems and wireless local positioning systems are now possible. 相似文献
85.
86.
Brett Jack Fontana Raymond P. Walsh Peter J. Thouret Wolfgang E. Gerber Max Kaufman Rudolph Thorington Luke 《Industry Applications, IEEE Transactions on》1981,(2):210-216
The design considerations for filaments in energy conserving incandescent lamps are described. Such lamps require compact high emissivity filaments to absorb infrared radiation reflected from heat mirrored focusing envelopes. The relationships between the electrical parameters in the radiation returning environment and in the clear surroundings are developed. Efficiency experiments with filaments of various geometries under conditions of radiation return were performed. A highly compact coiled coil filament seems most promising for development. A calculation is presented of the filament emissivity as a function of spacing of the windings in a coil. Measurements of the emissivity as a function of the pitch of a coiled coil filament agree with the calculation. 相似文献
87.
Julia Dittrich Susen Becker Max Hecht Uta Ceglarek 《Proteomics. Clinical applications》2015,9(1-2):5-16
The simultaneous quantification of protein concentrations via proteotypic peptides in human blood by liquid chromatography coupled to quadrupole MS/MS is an important field of bioanalytical research with a high potential for routine diagnostic applications. This review summarizes currently available sample preparation procedures and trends for absolute protein quantification in blood using LC-MS/MS. It discusses approaches of transferring established qualitative protocols to a quantitative analysis regarding their reliability and reproducibility. Techniques used to enhance method sensitivity such as the depletion of high-abundant proteins or the immunoaffinity enrichment of proteins and peptides are described. Furthermore, workflows for (i) protein denaturation, (ii) disulfide bridge reduction and (iii) thiol alkylation as well as (iv) enzymatic digestion for absolute protein quantification are presented. The main focus is on the tryptic digestion as a bottleneck of protein quantification via proteotypic peptides. Conclusively, requirements for a high-throughput application are discussed. 相似文献
88.
Abstract This paper discusses the applicability of the ideas of fuzzy sets and grades of membership to problems encountered in the quantification of clinical (i.e., diagnostic and prognostic) judgment. The methods of constrained maximum likelihood are used to derive consensus estimates of grades of membership given a set of categorical data and an a priori set of specified pure types. A numerical example is given. 相似文献
89.
Dr. Daniel Peitz Dr. Andreas M. Bernhard Dr. Max Mehring Martin Elsener Dr. Oliver Kröcher 《化学,工程师,技术》2013,85(5):625-631
In order to reduce the NOx concentration in car exhausts usually the selective catalytic reduction with ammonia is used. However, to avoid the transport of ammonia in vehicles urea is applied as NH3 precursor. Controlled urea decomposition before the injection into the exhaust gas system itself may be accomplished by the use of a separate reactor. Urea decomposition to ammonia in the liquid phase under pressure in a heated reactor was compared to its decomposition in the gas phase. In the liquid phase, higher conversion rates relative to the reactor volume were realized than in the gas phase. Catalysts which showed high activity in the gas phase influenced the decomposition in the liquid phase only slightly. 相似文献
90.