The establishment of the maximum permissible concentration of orlok in the air of a work area when used in agriculture

Inhaled Orlok causes changes in the CNS, blood morphology, renal function. The threshold concentration of Orlok is 0.17 mg/m3, its subthreshold concentration is 0.023 mg/m3, MAC in the workplace air is 0.05 mg/m3.     

Dendritic cells in the treatment of cancer

Thomas Berdmore's book, Disorders of Deformities of the Teeth and Gums, presents examples of early concepts of preventive dentistry and treatment of periodontal diseases. Various aspects of dental calculus formation and composition are analyzed. Also the effects of periodontal disease as seen by Berdmore are discussed, and ways of treatment in 1770 summarized.     

The puncture laser dissectomy application for the discogenic lumbosacral radiculitis

How many times have you been asked to participate in a meeting, forum, or task force to address a community health issue in the last few years? Opportunities seem to crop up almost weekly as non-profit organizations acclimate to meet changing needs and health systems continue to adapt to market forces. But participating in ongoing community projects, or even attending periodic meetings, draws time away from practice and professional obligations and limits an already modest supply of personal time. Is involvement in a community collaborative effort worth the time? And, if so, what are the benefits and challenges to physicians, health systems, and communities from this type of investment?     

The impact of blood culture reporting and clinical liaison on the empiric treatment of bacteraemia

AIMS: To assess the impact of blood culture results and early clinical liaison on the treatment of patients with bacteraemia. METHODS: 123 patients with significant positive blood cultures were followed over a nine month period in a 620 bed teaching hospital. The impact of early blood culture reporting and clinical liaison on the cost and appropriateness of treatment was assessed. RESULTS: Empiric treatment was started before the Gram stain result in 107 (87%) patients. Treatment was altered on the basis of the Gram stain result in 39 (36%) of these patients, and on culture and sensitivity results in 53 (50%). The spectrum of antibiotic treatment was narrowed in 58 (54%) of these; 20 (19%) on Gram stain result alone. This resulted in a 42% reduction in daily antibiotic costs in patients who had received empiric treatment. Empiric treatment did not follow the hospital antibiotic policy in 49 (46%) of the patients treated. In patients where empiric treatment was not in accordance with hospital policy, 21 (44%) had an isolate resistant to the empiric treatment used; while in patients who received agents in accordance with hospital policy only one (1.7%) had a resistant isolate (p < 0.05). Patients who died (11 (9%)) were less likely to have received empiric treatment in accordance with the antibiotic policy, although this did not reach statistical significance (p = 0.1). CONCLUSION: Early reporting of Gram stain results from blood cultures, combined with early clinical liaison, results in more rational and cost effective treatment.     

Yeast as a model system to study drugs effective against apicomplexan proteins

Biochemical and genetic analyses are required to identify potential drug targets in apicomplexan parasites, but these studies have proved difficult in most parasite systems. We have developed methods based on expression of parasite proteins in the budding yeast, Saccharomyces cerevisiae, to rapidly screen drugs directed against particular parasite targets, to study the structure and function of these target molecules, and to identify mutations in the parasite genes that alter enzyme specificity or drug sensitivity. In this paper we outline the parameters that need to be considered to design yeast strains that function efficiently to assay function of parasite proteins. Basic protocols and methods are included. We detail some problems that might be encountered in the engineering of these yeast strains and suggest possible solutions.