An 8-Bit Digitally Controlled Programmable Phase Shifter Circuit for Sinusoidal Signals with 252∘ Phase Control Range

In this paper, the synthesis, design, and implementation of a programmable phase shifter circuit for sinusoidal signals is presented. The proposed circuit, built-up herein with operational amplifiers (OPAMPs), high precision resistors and low voltage switches, consists of a digitally controlled amplitude attenuator in combination with a single-tone orthogonalizer. Experimental results agree with theoretical background: the attained phase range was 252^° in 256 steps with a median step of 0.9^°. The inaccuracy of the circuit was determined to be of 0.03 %. Contrary to other OPAMP approaches for sinusoidal signals reported in the literature and based on a first-order all-pass filter structure, the approximation suggested in this work is based on a different concept. The achieved results demonstrate the functionality of the system for the case of a sinusoidal signal with frequency of 1 kHz. Notwithstanding, the proposed architecture can be extended to operate at higher frequencies by using different building blocks with larger bandwidth. Furthermore, it can be extended as well to work out with other periodic input waveforms, like triangular shapes or square waves, with the use of an appropriate orthogonalizer.     

Contribution of the Tyr-1 in Plantaricin149a to Disrupt Phospholipid Model Membranes

Plantaricin149a (Pln149a) is a cationic antimicrobial peptide, which was suggested to cause membrane destabilization via the carpet mechanism. The mode of action proposed to this antimicrobial peptide describes the induction of an amphipathic α-helix from Ala7 to Lys20, while the N-terminus residues remain in a coil conformation after binding. To better investigate this assumption, the purpose of this study was to determine the contributions of the Tyr1 in Pln149a in the binding to model membranes to promote its destabilization. The Tyr to Ser substitution increased the dissociation constant (K_D) of the antimicrobial peptide from the liposomes (approximately three-fold higher), and decreased the enthalpy of binding to anionic vesicles from −17.2 kcal/mol to −10.2 kcal/mol. The peptide adsorption/incorporation into the negatively charged lipid vesicles was less effective with the Tyr1 substitution and peptide Pln149a perturbed the liposome integrity more than the analog, Pln149S. Taken together, the peptide-lipid interactions that govern the Pln149a antimicrobial activity are found not only in the amphipathic helix, but also in the N-terminus residues, which take part in enthalpic contributions due to the allocation at a lipid-aqueous interface.     

Seed oils of ten traditional Portuguese grape varieties with interesting chemical and antioxidant properties

To increase the potential and better exploring of grape seeds that are an important wine-industrial waste, oils of ten traditional Portuguese grape varieties were evaluated in relation to their vitamin E content (tocopherols and tocotrienols), fatty acid profile, as well as, antioxidant properties. Our results showed that the grape-seed oils were a good source of γ-tocotrienol (499–1575 mg/kg), α-tocopherol (85.5–244 mg/kg) and α-tocotrienol (69–319 mg/kg). Concerning fatty acid profile, linoleic (C18:2cc), oleic (C18:1), palmitic (C16:0) and stearic (C18:0) acids were the predominant. Grape-seed oils demonstrated to be a good source of polyunsaturated fatty acids (PUFAs) (63.64–73.53%), whereas monounsaturated fatty acid (MUFA) and saturated fatty acid (SFA) ranged between 14.19–21.29% and 11.64–14.94%, respectively. Interesting values of DPPH and ABTS radical scavenging activities were also obtained. This study demonstrated that these seeds may be reused and their oils incorporated in other food products, taking into account the compounds with positive effects on human health that are present in their composition.     

A model for the position and ring eccentricity in transverse sections of Norway spruce logs

=0.677, when the disc eccentricity is estimated by the model) and excellent (r²=0.923), when the disc eccentricity is measured and integrated as input in the model. This result motivates further studies concerning the automatic detection of the pith position by image analysis.     

A modeling approach to the effect of resin characteristics on parison formation in extrusion blow molding

The most critical stage in the extrusion blow‐molding process is the parison formation, as the dimensions of the blow‐molded part are directly related to the parison dimensions. The swelling due to stress relaxation and sagging due to gravity are strongly influenced by the resin characteristics, die geometry, and operating conditions. These factors significantly affect the parison dimensions. This could lead to a considerable amount of time and cost through trial and error experiments to get the desired parison dimensions based upon variations in the resin characteristics, die geometry, and operating conditions. The availability of a modeling technique ensures a more accurate prediction of the entire blow‐molding process, as the proper prediction of the parison formation is the input for the remaining process phases. This study considers both the simulated and the experimental effects of various high‐density polyethylene resin grades on parison dimensions. The resins were tested using three different sets of die geometries and operating conditions. The target parison length was achieved by adjusting the extrusion time for a preset die gap opening. The finite element software BlowParison® was used to predict the parison formation, taking into account the swell and sag. Good agreements were found between the predicted parison dimensions and the experimental data. POLYM. ENG. SCI., 2009. Published by Society of Plastics Engineers     

Proteolysis during the ripening of goats’ milk cheese made with plant coagulant or calf rennet

Powdered plant coagulant (PPC) obtained from the cardoon (Cynara cardunculus) was compared with calf rennet (CR) for the manufacture of goats’ milk cheese, by determining difference in the proteolysis throughout ripening. There were no substantial differences between the compositions of cheeses made using the two types of coagulants. However, cheeses manufactured with PPC exhibited higher levels of pH 4.6-SN than cheese made using CR. The extent of breakdown of α_s-casein, as measured by urea-PAGE, was greater in cheese made using PPC than cheese made using CR. The formation of hydrophobic peptides and the ratio of hydrophobic/hydrophilic peptides throughout the ripening were higher in cheeses made with PPC than in cheeses made with CR. Principal component analysis (PCA) of peak heights of RP-HPLC peptide profiles of the ethanol-soluble and ethanol-insoluble fractions distributed the samples according to the coagulant used in their manufacture. Quantitative differences in several peptides were evident between the two types of cheese.     

Generalized orderings of excess lifetimes of renewal processes

Several concepts of generalized orderings and generalized ageing classes have been considered in the literature (see Fagiuoli and Pellerey, 1993, Nav Res Logist 40: 829–842). They have been used in reliability, economics and actuarial sciences. These generalized notions provide a knowledge of the intrinsic structure of the ageing and ordering properties of random variables and have become an important tool in applied probability. In this paper, we provide new results about generalized orderings of excess lifetimes at different times of a renewal process when the underlying distribution belongs to some generalized ageing class. We also derive some interpretations and give some discussions of these results.