HSPA4 Is a Biomarker of Placenta Accreta and Enhances the Angiogenesis Ability of Vessel Endothelial Cells

Placenta accreta spectrum (PAS) accounts for 7% of maternal mortality and is associated with intraoperative and postoperative morbidity caused by massive blood loss, infection, and adjacent organ damage. The aims of this study were to identify the protein biomarkers of PAS and to further explore their pathogenetic roles in PAS. For this purpose, we collected five placentas from pregnant subjects with PAS complications and another five placentas from normal pregnancy (NP) cases. Then, we enriched protein samples by specifically isolating the trophoblast villous, deeply invading into the uterine muscle layer in the PAS patients. Next, fluorescence-based two-dimensional difference gel electrophoresis (2D-DIGE) and MALDI-TOF/MS were used to identify the proteins differentially abundant between PAS and NP placenta tissues. As a result, nineteen spots were determined as differentially abundant proteins, ten and nine of which were more abundant in PAS and NP placenta tissues, respectively. Then, specific validation with western blot assay and immunohisto/cytochemistry (IHC) assay confirmed that heat shock 70 kDa protein 4 (HSPA4) and chorionic somatomammotropin hormone (CSH) were PAS protein biomarkers. Further tube formation assays demonstrated that HSPA4 promoted the in vitro angiogenesis ability of vessel endothelial cells, which is consistent with the in vivo scenario of PAS complications. In this study, we not only identified PAS protein biomarkers but also connected the promoted angiogenesis with placenta invasion, investigating the pathogenetic mechanism of PAS.     

Synthesis, characterization and osteoconductivity properties of bone fillers based on alendronate-loaded poly(ε-caprolactone)/hydroxyapatite microspheres

A superior drug controlled release system capable of achieving efficient osteogenesis is in imperative demand because of limited bone substitute tissue for the treatment of bone defect. In the present study, we investigated the potential of using poly(ε-caprolactone)–hydroxyapatite (PCL–HA) composite microspheres as an injectable bone repair vehicle by controlled release of alendronate (AL), a medicine that belongs to the bisphosphonates family. The PCL/HA–AL microspheres were prepared with solid/oil/water emulsion technique, which included two processes: (1) AL was loaded on the hydroxyapatite nanoparticles; (2) the HA–AL complex was built in the PCL matrix. The spherical PCL/HA–AL microspheres were characterized with its significantly improved encapsulation efficiency of hydrophilic AL and better sustained release. Human bone mesenchymal stem cells (hMSCs) were cultured on the surface of these microspheres and exhibited high proliferative profile. Specifically, in osteogenic medium, hMSCs on the surface of PCL/HA–AL microspheres displayed superior osteogenic differentiation which was verified by alkaline phosphatase activity assay. In conclusion, by presenting strong osteogenic commitment of hMSCs in vitro, the PCL/HA–AL microspheres have the potential to be used as an injectable vehicle for local therapy of bone defect.     

基于Peercast的P2P直播系统改进

分析Peercast流媒体直播系统的结构。根据分析结果,指出其在实际网络应用中的弊端。改进下载流程,将单线程下载改为多线程下载,设计多线程F载任务分配模块。重新设计缓冲区,维护多对一情况F的缓冲区。介绍在多线程下载情况下直播延时方面的改进,对改进方案的性能进行分析。     

QoS-aware placement of stream processing service

Many emerging online stream processing services require the consideration of quality of service (QoS), which is highly dependent on the placement of services at various hosts. This paper investigates the QoS-aware placement problems of stream processing services under different contexts. On condition that the client demands are stable, the QoS-aware placement problem aiming to minimize the cost when servers are CPU-uncapacitated, is equivalent to the set cover problem, and can be solved by a greedy algorithm with approximation factor O(log?n), where n is the number of clients. However, when CPU capacity constraints on servers are taken into account, the QoS-aware placement problem cannot be approximated unless P=NP. Therefore, we propose two heuristic algorithms: (1) ISCA (Iterated Set Cover-based Algorithm) and (2) KBA (Knapsack-Based Algorithm). We also consider the placement problem of client demands increasing over time. Two objectives, called extension factor and system lifetime, are proposed for demand increment-blind and increment-aware models respectively. Both of them can be solved by extending ISCA and KBA. The experimental results show that ISCA and KBA have distinct effects on different demand sizes. ISCA is more efficient when client demands are relatively small, while KBA performs better for larger demands.     

基于分段地址结构的快速路由查找算法

根据IPV6地址结构和骨干路由表特点,分析了原有路由查找算法,基于IPV6的掩码长度和分段地址,采用Hash表和多分支Trie树结构,提出了一种快速的IPV6路由查找算法。根据分段地址和掩码将最常用到的路由前缀按前缀长度设置Hash表,并将前缀值有序存放在表结点中。不仅可以进行前缀长度的二分查找,同时又是其它前缀匹配的索引。对于其他的前缀匹配问题,根据Hash表中的索引到相应的多分支Trie树完成最长前缀匹配。实践证明该算法具有较好的时空效率,可以较好地提高路由查找速度。     

基于闭环矢量法的六足机器人关节位姿研究

设计出了一种新型的三自由度缩放式的并联机构,并把该机构作为六足步行机器人的腿机构.采用闭环矢量法求解腿机构运动位置的正运动学问题,用MATLAB软件编写函数来求解腿机构的最大腿行程和最大抬跨高度/研究表明,原动件较小的位移可以获得较大的足端运动空间.     

Hierarchical Magnetic Network Constructed by CoFe Nanoparticles Suspended Within “Tubes on Rods” Matrix Toward Enhanced Microwave Absorption

Hierarchical magnetic-dielectric composites are promising functional materials with prospective applications in microwave absorption(MA)field.Herein,a three-dimension hierarchical“nanotubes on microrods,”core–shell magnetic metal–carbon composite is rationally constructed for the first time via a fast metal–organic frameworksbased ligand exchange strategy followed by a carbonization treatment with melamine.Abundant magnetic CoFe nanoparticles are embedded within one-dimensional graphitized carbon/carbon nanotubes supported on micro-scale Mo2N rod(Mo2N@CoFe@C/CNT),constructing a special multi-dimension hierarchical MA material.Ligand exchange reaction is found to determine the formation of hierarchical magnetic-dielectric composite,which is assembled by dielectric Mo2N as core and spatially dispersed CoFe nanoparticles within C/CNTs as shell.Mo2N@CoFe@C/CNT composites exhibit superior MA performance with maximum reflection loss of−53.5 dB at 2 mm thickness and show a broad effective absorption bandwidth of 5.0 GHz.The Mo2N@CoFe@C/CNT composites hold the following advantages:(1)hierarchical core–shell structure offers plentiful of heterojunction interfaces and triggers interfacial polarization,(2)unique electronic migration/hop paths in the graphitized C/CNTs and Mo2N rod facilitate conductive loss,(3)highly dispersed magnetic CoFe nanoparticles within“tubes on rods”matrix build multi-scale magnetic coupling network and reinforce magnetic response capability,confirmed by the off-axis electron holography.     

Current–voltage characteristics of AlCdO Schottky contact on the polished and unpolished p-type Si surfaces with and without light illumination

In this study, the current-voltage characteristics of the AlCdO/unpolished p-type Si and AlCdO/polished p-type Si Schottky diodes with and without light illumination were examined. It is found that the Schottky barrier height (the series resistance) of the AlCdO/unpolished p-type Si Schottky diode is higher (lower) than that of the AlCdO/polished p-type Si Schottky diode. The power conversion efficiency of the AlCdO/p-type Si devices in the light (AM 1.5 G, 100 mW/cm²) was improved by increasing built-in potential at the AlCdO/p-type Si interfaces and reducing the device series resistance and surface reflectivity. It is shown that the device surface roughness plays an essential role in improving the device performance.     

Dieless drawing steel wires using a dielectric heating method and modeling the process dynamics

Modern industries require the production of multi-functional, inorganic, micron-sized metal wires. This study suggests a novel method that could potentially offer a highly efficient dieless drawing technology for manufacturing thin stainless steel fibers. The method is based on a hot-working principle, using microwaves as the heat source and SiC as the susceptor. Experimental trials with a laboratory rig showed that the new system worked effectively for drawing the stainless steel wires and should be able to realize the diameter attenuation with a diameter reduction of up to 21%. The theoretical model describing the deformation behavior of the stainless steel wires in the working zone along with the constitutive equation of Bingham model modified with a power law and Zener–Hollomon parameter turned out to match very good with the actual results of the experiment. The coefficient of variation of the drawn wire diameter increased, as the draw ratio increased, which could be attributed to the occurrence of the narrow necking zone.