Real-Time Monitoring the Effect of Cytopathic Hypoxia on Retinal Pigment Epithelial Barrier Functionality Using Electric Cell-Substrate Impedance Sensing (ECIS) Biosensor Technology

Disruption of retinal pigment epithelial (RPE barrier integrity is a hallmark feature of various retinal blinding diseases, including diabetic macular edema and age-related macular degeneration, but the underlying causes and pathophysiology are not completely well-defined. One of the most conserved phenomena in biology is the progressive decline in mitochondrial function with aging leading to cytopathic hypoxia, where cells are unable to use oxygen for energy production. Therefore, this study aimed to thoroughly investigate the role of cytopathic hypoxia in compromising the barrier functionality of RPE cells. We used Electric Cell-Substrate Impedance Sensing (ECIS) system to monitor precisely in real time the barrier integrity of RPE cell line (ARPE-19) after treatment with various concentrations of cytopathic hypoxia-inducing agent, Cobalt(II) chloride (CoCl₂). We further investigated how the resistance across ARPE-19 cells changes across three separate parameters: R_b (the electrical resistance between ARPE-19 cells), α (the resistance between the ARPE-19 and its substrate), and C_m (the capacitance of the ARPE-19 cell membrane). The viability of the ARPE-19 cells and mitochondrial bioenergetics were quantified with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and seahorse technology, respectively. ECIS measurement showed that CoCl₂ reduced the total impedance of ARPE-19 cells in a dose dependent manner across all tested frequencies. Specifically, the ECIS program’s modelling demonstrated that CoCl₂ affected R_b as it begins to drastically decrease earlier than α or C_m, although ARPE-19 cells’ viability was not compromised. Using seahorse technology, all three concentrations of CoCl₂ significantly impaired basal, maximal, and ATP-linked respirations of ARPE-19 cells but did not affect proton leak and non-mitochondrial bioenergetic. Concordantly, the expression of a major paracellular tight junction protein (ZO-1) was reduced significantly with CoCl_2-treatment in a dose-dependent manner. Our data demonstrate that the ARPE-19 cells have distinct dielectric properties in response to cytopathic hypoxia in which disruption of barrier integrity between ARPE-19 cells precedes any changes in cells’ viability, cell-substrate contacts, and cell membrane permeability. Such differences can be used in screening of selective agents that improve the assembly of RPE tight junction without compromising other RPE barrier parameters.     

Gurum Seeds: A Potential Source of Edible Oil

Cucurbitaceae family seeds are mostly discarded as agro-industrial wastes. Gurum (Citrullus lanatus var. colocynthoide) is an underutilized wild cucurbit plant, closely related to desert watermelon, which is grown abundantly in some African countries. Gurum seeds can play a significant role in health and nutrition due to their high oil content. This review describes the nutritional composition of gurum seeds and their oil profile. Gurum seeds are a good source of oil (27–35.5%), fiber (26–31%), crude protein (15–18%), and carbohydrates (14–17%). Gurum seeds oil is extracted by supercritical CO₂ (SFE), screw press, and solvent extraction techniques. The gurum seeds oil is composed of unsaturated fatty acids with a high proportion of linoleic acid (C18:2) and oleic acid (C18:1). Gurum seeds oil contains various bioactive compounds, such as tocopherols, phytosterols, and polyphenols. It is reported that solvent extraction gives a higher yield than the screw press and SFE, but the SFE is preferred due to safety issues. More studies are required for producing better quality gurum seeds oil by using novel extraction techniques that can increase oil yield.     

Combustion synthesis of NbC/ZrO2 composites: Influence of Cr additives on the microstructure and mechanical properties

Composites of NbC/ZrO₂ reinforced with different weight ratios of Cr metal were prepared by dynamic compaction combustion from a blend of Nb₂O₅–Zr–Cr powders. Factors controlling the synthesis process, the microstructure, and the mechanical properties of the samples, such as Cr wt% and the compression loads were studied. The samples were characterized by XRD, SEM, porosity, and hardness measurements. The porosity value of the sample with no additives was high and reached 31.6 vol%. A sudden decrease in the sample porosity to 2.4 vol % was noticed for the sample containing 3.0 wt% of Cr. A high-density sample with less than 2.0 vol % porosity and maximum hardness of 1038 HV was produced using 5.0 wt% Cr under 300 MPa compression load. Detailed thermodynamic calculations for the effect of Cr additives on the physicochemical properties of the system were introduced.     

The Impact of Metastable Intermolrecular Nanocomposite Particles on Kinetic Decomposition of Heterocyclic Nitramines Using Advanced Solid‐Phase Decomposition Models

Journal of Inorganic and Organometallic Polymers and Materials - Surface oxygen of oxide catalyst has low coordination number; they are negatively charged. Surface oxygen can act active site for...     

Electrochemical behaviour of Mn3+/Mn2+, Co3+/Co2+ and Ce4+/Ce4+ redox mediators in methanesulfonic acid

The redox behaviour of Mn³⁺/Mn²⁺, Co³⁺/Co²⁺ and Ce⁴⁺/Ce³⁺ mediators commonly used in indirect oxidation of organic compounds were evaluated in methane sulfonic acid on a glassy carbon working electrode employing cyclic voltammetry. Manganic methanesulfonate exhibits higher instability in dilute methanesulfonic acid. The solid MnO₂ formed during disproportionation on the glassy carbon electrode further affects the reproducibility. Cobaltic methanesulfonate formation occurs only at oxygen evolution region rendering the overall oxidation process less efficient. Ceric methane sulfonate formation is highly efficient over a wide acid concentration range. Ceric methanesulfonate can also be employed over a wide temperature range to oxidize different aromatic compounds.     

The lime-silica-water system: Equilibria at bulk calcium/silicon ratios of 1·5–2·5 AT 180–250°C

The stability relationships of xonotlite, foshagite and hillebrandite relative to kilchoanite and calcio-chondrodite have been studied under saturated steam pressures at 180° and 250°. Kilchoanite was unstable relative to xonotlite plus hillebrandite at 180°, or to foshagite plus hillebrandite at 250°; calcio-chondrodite was unstable relative to hillebrandite plus some more lime-rich phase, possibly Ca(OH)₂, at both temperatures. Foshagite was stable relative to xonotlite plus hillebrandite at 250°, but unstable relative to these phases at 180°.     

Barium aluminate hydrates. IV. α-BaO.Al2O3.4H2O

α-BaO.Al₂O₃.4H₂O has been synthesised and studied by infrared, X-ray and thermal analytical techniques. The compound of approximate formula BaO.Al₂O₃.0.5H₂O, described in Part III, forms as a dehydration product, and appears to be identical with the compound β-BAH₂ described by other workers. A possible explanation for the discrepancy in water content is discussed.     

PRIVACY LEAKAGE IN HEALTH SOCIAL NETWORKS

Members of health social networks may be susceptible to privacy leaks by the amount of information they leave behind. The threat to privacy increases when members of these networks reuse their pseudonyms in other social networks. The risk of re‐identifying users from such networks requires quantitative estimates to evaluate its magnitude. The estimates will enable managers and members of health social communities to take corrective measures. We introduce a new re‐identification attack, the social network attack, that takes advantage of the fact that users reuse their pseudonyms. To demonstrate the attack, we establish links between MedHelp and Twitter (two popular social networks) based on matching pseudonyms. We used Bayesian networks to model the re‐identification risk and used stylometric techniques to identify the strength of the links. On the basis of our model 7‐11. 8% of the MedHelp members in the sample population who reused their pseudonyms in Twitter were re‐identifiable compared with 1% who did not. The risk estimates were measured at the 5% risk threshold. Our model was able to re‐identify users with a sensitivity of 41% and specificity of 96%. The potential for re‐identification increases as more data is accumulated from these profiles, which makes the threat of re‐identification more serious.     

Molecular solutions for minimum and exact cover problems in the tile assembly model

The tile assembly model is a novel biological computing model where information is encoded in DNA tiles. It is an efficient way to solve NP-complete problems due to its scalability and parallelism. In this paper, we apply the tile assembly model to solve the minimum and exact set cover problems, which are well-known NP-complete problems. To solve the minimum set cover problem, we design a MinSetCover system composed of three parts, i.e., the seed configuration subsystem, the nondeterministic choice subsystem, and the detection subsystem. Moreover, we improve the MinSetCover system and propose a MinExactSetCover system for solving the problem of exact cover by 3-sets. Finally we analyze the computation complexity and perform a simulation experiment to verify the effectiveness and correctness of the proposed systems.     

Effect of Product Conductivity on Field-Activated Combustion Synthesis

Experimental and modeling results on the field-activated combustion synthesis (FACS) of Nb₅Si₃ are reported. In the absence of an electric field and without reactant preheating, Nb₅Si₃ cannot be prepared by self-propagating high-temperature synthesis (SHS). Under the influence of a field a self-sustaining combustion wave is established whose rate of propagation decreases with traveled distance. For relatively low field values, the wave propagation mode changes from a continuous (smooth) to a spin mode. The product of synthesis depends on the mode of propagation. Synthesis during continuous wave propagation results in the formation of Nb₅Si₃, primarily in the α-modification. In contrast, when the wave propagates in a spin mode, the product is NbSi₂ with unreacted niobium. The present observations demonstrate a case where the field effect is not localized, as was the case in previous studies. The difference in behavior is attributed to differences in the electrical conductivities of the product phases.