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991.
AJ Pierik W Roseboom RP Happe KA Bagley SP Albracht 《Canadian Metallurgical Quarterly》1999,274(6):3331-3337
Infrared-spectroscopic studies on the [NiFe]-hydrogenase of Chromatium vinosum-enriched in 15N or 13C, as well as chemical analyses, show that this enzyme contains three non-exchangeable, intrinsic, diatomic molecules as ligands to the active site, one carbon monoxide molecule and two cyanide groups. The results form an explanation for the three non-protein ligands to iron detected in the crystal structure of the Desulfovibrio gigas hydrogenase (Volbeda, A., Garcin, E., Piras, C., De Lacey, A. I., Fernandez, V. M., Hatchikian, E. C., Frey, M., and Fontecilla-Camps, J. C. (1996) J. Am. Chem. Soc. 118, 12989-12996) and for the low spin character of the lone ferrous iron ion observed with M?ssbauer spectroscopy (Surerus, K. K., Chen, M., Van der Zwaan, W., Rusnak, F. M., Kolk, M. , Duin, E. C., Albracht, S. P. J., and Münck, E. (1994) Biochemistry 33, 4980-4993). The results do not support the notion, based upon studies of Desulfovibrio vulgaris [NiFe]-hydrogenase (Higuchi, Y., Yagi, T., and Noritake, Y. (1997) Structure 5, 1671-1680), that SO is a ligand to the active site. The occurrence of both cyanide and carbon monoxide as intrinsic constituents of a prosthetic group is unprecedented in biology. 相似文献
992.
The general principles and experimental difficulties associated with the measurement of permeability in aluminum alloys have
been discussed, in order to ease the further development of experimental methods suitable for such measurements. In order
to avoid the surface-tension effects associated with liquid/gas interfaces in the narrow channels of the mushy zone, a flux
melt can be used at the inlet of the permeameter. Electron-beam (EB) welding has been shown to be a suitable method for ensuring
a complete wetting between the sample and flux after remelting to the test temperature. Moreover, the influence of the permeameter
design and the experimental procedure on grain detachment, preferred-flow channel formation, and coarsening have been revealed
through microstructural images. 相似文献
993.
Matthias Heymann Janet Martin‐Nielsen 《Centaurus; international magazine of the history of science and medicine》2013,55(3):221-242
With this introduction we aim to illuminate Western Europe's place on the map of Cold War science and, specifically, to draw attention to the differences in and the diversity of Western European Cold War science in comparison to the United States. By discussing narratives of Cold War science in small states and asking how they fit into the European condition, we suggest that the fact of being a small state affects the conditions for and the scope of Cold War science. As a whole, this special issue also emphasizes the importance of the spatial dimension; that is, the significant dependence of Cold War science on geographical relations and geopolitical interests. 相似文献
994.
Mean outcrossing rates can be used as a basis for decision support for ships in severe sea. The article describes a procedure for calculating the mean outcrossing rate of non-Gaussian processes with stochastic input parameters. The procedure is based on the first-order reliability method (FORM) and stochastic parameters are incorporated by carrying out a number of FORM calculations corresponding to combinations of specific values of the stochastic parameters. Subsequently, the individual FORM calculation is weighted according to the joint probability with which the specific combination of parameter values is expected to occur, and the final result, the mean outcrossing rate, is obtained by summation. The derived procedure is illustrated by an example considering the forces in containers stowed on ships and, in particular, results are presented for the so-called racking failure in the containers. The results of the procedure are compared with brute force simulations obtained by Monte Carlo simulation (MCS) and good agreement is observed. Importantly, the procedure requires significantly less CPU time compared to MCS to produce mean outcrossing rates. 相似文献
995.
Nielsen T.N. Eggleton B.J. Rogers J.A. Westbrook P.S. Hansen P.B. Strasser T.A. 《Photonics Technology Letters, IEEE》2000,12(2):173-175
A compact tunable fiber Bragg grating that uses distributed thin-film heaters on the surface of the fiber is used to dynamically optimize the post dispersion compensation of a multi-span 40-Gb/s nonreturn-to-zero (NRZ) transmission system. Dynamic post dispersion compensation with this device enables the system to operate over a much wider range of launch power than is otherwise possible with simple, fixed compensation using dispersion compensating fiber 相似文献
996.
997.
High-entropy alloys are formed by synthesizing five or more principal elements in equimolar or near equimolar concentrations. Microstructure of the CoCrCuFeNiAl0.5Bx (x = 0, 0.2, 0.6, 1) high-entropy alloys under investigation is composed of a mixture of disordered bcc and fcc phases and borides. These alloys were tested gravimetrically for their corrosion resistance in simulated syngas containing 0, 0.01, 0.1, and 1 % H2S at 500 °C. The exposed coupons were characterized using XRD and SEM. No significant corrosion was detected at 500 °C in syngas containing 0 and 0.01 % H2S while significant corrosion was observed in syngas containing 0.1 and 1 % H2S. Cu1.96S was the primary sulfide in the external corrosion scale on the low-boron high-entropy alloys, whereas FeCo4Ni4S8 on the high-boron high-entropy alloys. Multi-phase Cu-rich regions in the low-B high-entropy alloys were vulnerable to corrosive attack. 相似文献
998.
Erzeng Xue Julian R. H. Ross Reyes Mallada Miguel Menendez Jesus Santamaria Jens Perregard Poul E. Hojlund Nielsen 《Applied Catalysis A: General》2001,210(1-2):271-274
The addition of CO2 to the reaction mixture for the oxidation of butane over a VPO catalyst gives rise to significantly improved yields of maleic anhydride. 相似文献
999.
Despite having been relegated to the realm of superstition during the dominant years of behaviorism, the investigation and discussion of consciousness has again become scientifically defensible. However, attempts at describing animal consciousness continue to be criticized for lacking independent criteria that identify the presence or absence of the phenomenon. William James recognized that mental traits are subject to the same evolutionary processes as are physical characteristics and must therefore be represented in differing levels of complexity throughout the animal kingdom. James's proposals with regard to animal consciousness are outlined and followed by a discussion of 3 classes of animal consciousness derived from empirical research. These classes are presented to defend both James's proposals and the position that a theory of animal consciousness can be scientifically supported. It is argued that by using particular behavioral expressions to index consciousness and by providing empirical tests by which to elicit these behavioral expressions, a scientifically defensible theory of animal consciousness can be developed. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
1000.
Methods are described for isolating smooth muscle cells from the tracheae of adult and neonatal sheep and measuring the single-cell shortening velocity. Isolated cells were elongated, Ca2+ tolerant, and contracted rapidly and substantially when exposed to cholinergic agonists, KCl, serotonin, or caffeine. Adult cells were longer and wider than preterm cells. Mean cell length in 1.6 mM CaCl2 was 194 +/- 57 (SD) microm (n = 66) for adult cells and 93 +/- 32 microm (n = 20) for preterm cells (P < 0.05). Mean cell width at the widest point of the adult cells was 8.2 +/- 1.8 microm (n = 66) and 5.2 +/- 1.5 microm (n = 20) for preterm cells (P < 0.05). Cells were loaded into a perfusion dish maintained at 35 degreesC and exposed to agonists, and contractions were videotaped. Cell lengths were measured from 30 video frames and plotted as a function of time. Nonlinear fitting of cell length to an exponential model gave shortening velocities faster than most of those reported for airway smooth muscle tissues. For a sample of 10 adult and 10 preterm cells stimulated with 100 microM carbachol, mean (+/- SD) shortening velocity of the preterm cells was not different from that of the adult cells (0.64 +/- 0.30 vs. 0.54 +/- 0.27 s-1, respectively), but preterm cells shortened more than adult cells (68 +/- 12 vs. 55 +/- 11% of starting length, respectively; P < 0.05). The preparative and analytic methods described here are widely applicable to other smooth muscles and will allow contraction to be studied quantitatively at the single-cell level. 相似文献