Development of a Multi-Epitope Vaccine for Mycoplasma hyopneumoniae and Evaluation of Its Immune Responses in Mice and Piglets

Mycoplasma hyopneumoniae (Mhp), the primary pathogen causing Mycoplasma pneumonia of swine (MPS), brings massive economic losses worldwide. Genomic variability and post-translational protein modification can enhance the immune evasion of Mhp, which makes MPS prone to recurrent outbreaks on farms, even with vaccination or other treatments. The reverse vaccinology pipeline has been developed as an attractive potential method for vaccine development due to its high efficiency and applicability. In this study, a multi-epitope vaccine for Mhp was developed, and its immune responses were evaluated in mice and piglets. Genomic core proteins of Mhp were retrieved through pan-genome analysis, and four immunodominant antigens were screened by host homologous protein removal, membrane protein screening, and virulence factor identification. One immunodominant antigen, {"type":"entrez-protein","attrs":{"text":"AAV27984.1","term_id":"53987783","term_text":"AAV27984.1"}}AAV27984.1 (membrane nuclease), was expressed by E. coli and named rMhp597. For epitope prioritization, 35 B-cell-derived epitopes were identified from the four immunodominant antigens, and 10 MHC-I and 6 MHC-II binding epitopes were further identified. The MHC-I/II binding epitopes were merged and combined to produce recombinant proteins MhpMEV and MhpMEVC6His, which were used for animal immunization and structural analysis, respectively. Immunization of mice and piglets demonstrated that MhpMEV could induce humoral and cellular immune responses. The mouse serum antibodies could detect all 11 synthetic epitopes, and the piglet antiserum suppressed the nuclease activity of rMhp597. Moreover, piglet serum antibodies could also detect cultured Mhp strain 168. In summary, this study provides immunoassay results for a multi-epitope vaccine derived from the reverse vaccinology pipeline, and offers an alternative vaccine for MPS.     

Spatial distribution of laser energy and its influence on the stability of extreme narrow keyholes during ultra-high power laser welding

The instability of extremely narrow keyholes is the main challenge in high-power laser welding.In this work,the spatial energy distribution and its influence on the stability of extremely narrow keyholes during ultra-high power laser welding are studied.A multiphase flow model that considers the vapor plume impact and the multiple scattering of laser radiation is built to simulate the topology and downstream wave flow on the keyhole wall.Drastic keyhole fluctuation is due to excessive energy acc...     

亭台楼阁类建筑的文学价值

亭台楼阁作为中国古代的传统建筑,其特殊的体式、功能及其与自然环境所构成的独特意境,成为历代文人登高临远的重要场所,形成了大量诗文,这些作品往往寄寓了游子怀乡、壮士悲歌、文士不遇等情感,许多名篇对后人产生了强大的精神感召力,成为中华民族精神文化遗产的一部分。     

ZnO薄膜的研究进展

光泵浦紫外激光的获得使ＺｎＯ成为热门的新型光电材料,文章从ＺｎＯ的基本特性、光电特性、生长方法,用途和应用前景等方面综述了ＺｎＯ的最新研究及进展,并对ＺｎＯ今后的发展作了展望。     

Insights into the Reaction Network and Mechanism of Green Aerobic Oxidative Esterification of Methacrolein over Different Heterogeneous Catalysts

The oxidative esterification of methacrolein (MAL) is an important way to prepare high-valued methyl methacrylate (MMA), but this process is ultra-complex due t...     

东濮凹陷砂岩储集层物性参数关系研究及随钻应用方法

储集层的物性参数是评价储集层的重要基础数据,对储集层评价的准确程度有较大影响,文章统计分析结果表明砂岩储集层岩石密度与储集层主要物性参数之间有良好的相关性。以区域统计规律为基础,建立了东濮凹陷砂岩储集层体积密度与孔隙度和渗透率的关系方程。介绍了录井施工现场应用岩屑样品体积置换法、泥岩密度计法两种常规检测方法,能较准确的获取砂岩储集层的体积密度,反演计算孔隙度和渗透率数据,合理评价储集层物性参数,为准确评价储集层提供了可信度高的重要基础数据,从而弥补了录井现场物性参数获取不足的缺陷,在随钻评价油气储集层过程中有一定的实用价值。     

Optical coherent dot-product chip for sophisticated deep learning regression

Optical implementations of neural networks (ONNs) herald the next-generation high-speed and energy-efficient deep learning computing by harnessing the technical...     

Hydrogen Bond-Assisted Ultra-Stable and Fast Aqueous NH4+ Storage

Aqueous ammo-nium ion batteries are regarded as eco-friendly and sustainable energy storage systems.And appli-cable host for NH4+ in aqueous solution is always ...     

多服务器环境下基于扩展混沌映射的认证密钥协商协议

传统的单服务器环境下基于智能卡认证方案,单个服务器对所有的注册远程用户提供服务。如果用户想要从不同的服务器获得网络服务,则必须分别在不同的服务器注册。为解决以上问题,研究者提出了多服务器认证方案。然而,文献中的大部分方案都不能实现强安全特性。受到切比雪夫映射的半群特性和基于扩展混沌映射的密钥协商协议启发,本文提出一种多服务器环境中的认证方案。新方案不需要使用验证表并且允许用户访问不同的服务器而不需要分别注册。新方案不仅可以抵抗各类攻击,还实现了用户的强匿名性。和以前的相关协议相比,新协议具有高效性和安全性,因而适合在实际环境中应用。     

一体式活塞内模结构设计及应用

采用一体式内模代替原来的两体式内模铸造模具,简化了模具制造工艺、节约了制造成本、美化了活塞内腔。利用Magma软件模拟浇注,发现一体式内模容易出现的缺陷以及缺陷存在的位置,并提出有效的解决方法。通过在小缸径活塞上的实际应用,取得了良好的效果。