筛查基因突变的新技术——循环双脱氧指纹图谱法及其应用

建立了适用于大批量标本基因突变筛查的双脱氧指纹图谱法即ｄｄＦ。该技术民测定ＤＮＡ序列的双脱氧末端终止法及ＳＳＣＰ二者长处于一身，即只作一个双脱氧末端终止反应，然后将反应产物在与ＳＳＣＰ相同的电泳条件下进行聚丙烯酰胺凝胶电泳，这样有突变的样品不会漏检，阳性检出率高达１００％。     

Fischer-Tropsch synthesis on Fe-Mn ultrafine catalysts

The Fischer-Tropsch (FT) synthesis on Fe-Mn ultrafine catalysts prepared by a special degradation method of Fe-Mn complexes is presented. The effects of preparation method and Mn content on the FT performance are examined and the active phases and the role of Mn are elucidated.     

天然调味料──酵母抽提物的研究进展

着重论述了酵母抽提物的特性、在食品中的应用前景、制造方法、工业化生产中存在的问题及其解决方法，并对酵母抽提物的发展方向作一展望。     

被Kerr介质包围的J—C模型中光场位相特性

本文应用Pegg-Barnett位相理论讨论了被Kerr介质包围的J-C模型中光场的位相性质。     

Ab initio calculations on the mechanism of isobutane and 2-butene alkylation reaction catalyzed by hydrofluoric acid

Ab initio method was employed to determine the mechanism of alkylation reaction of 2-butene with isobutane. The results indicated that 2-butene is rapidly protonated to form sec-butyl carbonium ion, which then reacts with isobutane to form tert-butyl carbonium ion. The resulting carbonium ion is then deprotonated to form isobutene that reacts rapidly with tert-butyl carbonium ion to form trimethylpentane (TMP) carbonium ion. Finally, the TMP⁺ carbonium ion is converted to TMP via the hydride transfer process. The transition states of the interactions were obtained by using the SCAN method (a calculation key word for potential energy surface) first and then the intrinsic reaction coordinates were traced using the Gaussian program. In addition, the energy changes and geometric parameters of the interactions were analyzed. The computed results indicated that the energy barriers are close to or less than zero for the interaction of isobutane with protonated 2-butene or for the interaction of tert-butyl carbonium ion with isobutene, suggesting rapid reaction steps of the carbonium ions. The 2,2,3-TMP is kinetically controlled, while the formation of 2,2,4-TMP is controlled by reaction equilibrium. The calculated results were in good agreement with the experimental results.     

Electron microscopic observations and DNA chain fragmentation studies on apoptosis in bone tumor cells induced by ~(153)Sm-EDTMP

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贝氏体钢内水冷穿孔顶头的粘钢及防止措施

从顶头生产工艺控制的角度,分析了贝氏体钢内水冷穿孔顶头产生粘钢的原因、特点和机制,指出掉鼻、缺肉是顶头粘钢的另一种更为严重的失效形式,强化熔炼工艺控制和保证夹杂物总量在A0.5B1C0.5D1级水平,粘钢问题可以得到有效控制。     

军械调运方案优化算法的设计与仿真

军械调运问题的数学模型和手工算法过去已有研究,但是当问题较为复杂时,手工算法就要花费大量时间而且准确性也难以保证。针对这一缺陷,本文在已有的数学模型和手工算法的基础上,设计了一种可以用计算机实现的基于递归的算法来寻求军械紧急调运问题的最优方案,并使用面向对象的java语言将算法实现。     

万安水利枢纽底孔坝段中隔墙流激振动响应分析

 研究了万安水利枢纽底孔坝段中隔墙在水流脉动壁压作用下的结构随机振动问题，进行了一种泄流工况下的隔墙结构流激振动响应统计量的理论计算，模拟计算了隔墙－水流－基础耦合系统固有频率对流激振动响应的影响，并对水流脉动壁压随机荷载作用下的结构动力可靠度进行了理论分析。