The two-spin model of a tracking chamber: a phase-space perspective

We study the dynamics of classical localization in a simple, one-dimensional model of a tracking chamber. The emitted particle is represented by a superposition of Gaussian wave packets moving in opposite directions, and the detectors are two spins in fixed, opposite positions with respect to the central emitter. At variance with other similar studies, we give here a phase-space representation of the dynamics in terms of the Wigner matrix of the system. This allows a better visualization of the phenomenon and helps in its interpretation. In particular, we discuss the relationship of the localization process with the properties of entanglement possessed by the system.

     

Genetic Architecture and Molecular,Imaging and Prodromic Markers in Dementia with Lewy Bodies: State of the Art,Opportunities and Challenges

Dementia with Lewy bodies (DLB) is one of the most common causes of dementia and belongs to the group of α-synucleinopathies. Due to its clinical overlap with other neurodegenerative disorders and its high clinical heterogeneity, the clinical differential diagnosis of DLB from other similar disorders is often difficult and it is frequently underdiagnosed. Moreover, its genetic etiology has been studied only recently due to the unavailability of large cohorts with a certain diagnosis and shows genetic heterogeneity with a rare contribution of pathogenic mutations and relatively common risk factors. The rapid increase in the reported cases of DLB highlights the need for an easy, efficient and accurate diagnosis of the disease in its initial stages in order to halt or delay the progression. The currently used diagnostic methods proposed by the International DLB consortium rely on a list of criteria that comprises both clinical observations and the use of biomarkers. Herein, we summarize the up-to-now reported knowledge on the genetic architecture of DLB and discuss the use of prodromal biomarkers as well as recent promising candidates from alternative body fluids and new imaging techniques.     

Proinflammatory Interleukin-33 Induces Dichotomic Effects on Cell Proliferation in Normal Gastric Epithelium and Gastric Cancer

Interleukin (IL)-33 is a member of the interleukin (IL)-1 family of cytokines linked to the development of inflammatory conditions and cancer in the gastrointestinal tract. This study is designed to investigate whether IL-33 has a direct effect on human gastric epithelial cells (GES-1), the human gastric adenocarcinoma cell line (AGS), and the gastric carcinoma cell line (NCI-N87) by assessing its role in the regulation of cell proliferation, migration, cell cycle, and apoptosis. Cell cycle regulation was also determined in ex vivo gastric cancer samples obtained during endoscopy and surgical procedures. Cell lines and tissue samples underwent stimulation with rhIL-33. Proliferation was assessed by XTT and CFSE assays, migration by wound healing assay, and apoptosis by caspase 3/7 activity assay and annexin V assay. Cell cycle was analyzed by means of propidium iodine assay, and gene expression regulation was assessed by RT-PCR profiling. We found that IL-33 has an antiproliferative and proapoptotic effect on cancer cell lines, and it can stimulate proliferation and reduce apoptosis in normal epithelial cell lines. These effects were also confirmed by the analysis of cell cycle gene expression, which showed a reduced expression of pro-proliferative genes in cancer cells, particularly in genes involved in G0/G1 and G2/M checkpoints. These results were confirmed by gene expression analysis on bioptic and surgical specimens. The aforementioned results indicate that IL-33 may be involved in cell proliferation in an environment- and cell-type-dependent manner.     

Film Blowing of Biodegradable Polymer Nanocomposites for Agricultural Applications

Films for agricultural applications, such as greenhouses films or mulching films are generally made of polyolefins such as linear low-density polyethylene (LLDPE) or low-density polyethylene. However, the use of biodegradable and/or compostable polymers is increasing, which enjoy the additional advantage that they can be left on the site since a fine life would be gradually assimilated to the underlying soil. Nevertheless, biodegradable polymeric films often do not have suitable mechanical performances. In this work, biodegradable polymer-based nanocomposite films are prepared by film blowing and compared with traditional LLDPE based nanocomposites. In particular, a biodegradable polymer blend and two different inorganic nanofillers (an organo-modified clay and a calcium carbonate with a hydrophobic coating) are used for the preparation of the nanocomposites. A detailed investigation of obtained materials is performed through rheological, mechanical, and optical characterizations. Adding nanofillers led to an increase of rigidity and tear strength of blown films without negatively affecting their ductility.     

Rationally designed inhibitors as tools for comparing the mechanism of squalene-hopene cyclase with oxidosqualene cyclase

The inhibition of squalene-hopene cyclase (SHC) (E.C. 5.4.99.-), an enzyme of bacterial membranes catalyzing the formation of pentacyclic sterol-like triterpenes, was studied by using different classes of compounds originally developed as inhibitors of oxidosqualene cyclase (OCS) (E.C. 5.4.99.7), the enzyme of eukaryotes responsible for the formation of tetracyclic precursors of sterols. The mechanism of cyclization of squalene by SHC, beginning with a protonation of the 2,3 double bond by an acidic residue of the enzyme, followed by a series of electrophilic additions of the carbocationic intermediates to the double bonds, is similar to the mechanism of cyclization of 2,3-oxidosqualene by OSC. The inhibitors studied included: (i) analogs of the carbocationic intermediates formed during cyclization, such as aza-analogs of squalene and 2,3-oxidosqualene; (ii) affinity-labeling inhibitors bearing a methylidene reactive group; and (iii) vinyldioxidosqualenes and vinylsulfide derivatives of the substrates. Comparison of the results obtained with the two enzymes, SHC and OSC, showed that many of the most effective inhibitors of OSC were also able to inhibit SHC, while some derivatives acted as specific inhibitors. Differences could be easily explained on the basis of the different substrate specificity of the two enzymes.     

Steady state multiplicity depending on coalescence in liquid—liquid continuous stirred reactors with reaction in the dispersed phase

The influence of drop coalescence and breakup on the existence of multiple steady states is studied for a two-phase stirred isothermal reactor where the chemical reaction in the d?ispersed phase obeys the rate expression ? r = kC/(1 + KC)². The random coalescence model developed by Curl was simulated using a modified Spielman and Levenspiel Monte Carlo technique.For certain range of the coalescence rate, Damköhler number, and dimensionless feed concentration, multiple steady states have been investigated.A special case has also been considered wherein the existence of multiple steady states for finite values of the coalescence rate is contrasted to the unique steady state solution for an infinite coalescence rate.     

Elastomeric properties of ethylene/propylene copolymers prepared by zirconocene/methylaluminoxane catalysts

Ethylene/propylene copolymers (EPM) have been prepared by a liquid propylene suspension process, using homogeneous catalysts based on nonbridged zirconocenes and methylaluminoxane (MAO). When bis(η⁵-cyclopentadienyl)zirconium dichloride (Cp₂ZrCl₂), bis(η⁵-cyclopentadienyl) dimethyl zirconium (Cp₂ZrMe₂) and bis(η⁵-cyclopentadienyl), dibenzyl zirconium (Cp₂ZrBz₂) were used as catalyst components, EPM having low average molecular weight (MW) were in general obtained in low yield. On the contrary, a very large improvement of productivity and MW was observed when bis(η⁵-indenyl)zirconium dichloride (Ind₂ZrCl₂) and bis(η⁵-indenyl) dimethyl zirconium (Ind₂ZrMe₂) as well as bis(η⁵-tetrahydroindenyl)zirconium dichloride [(IndH₄)₂ZrCI₂] were employed in combination with MAO. In particular, this last catalytic system allowed one to obtain EPM with a narrower distribution of MW in addition to the above characteristics. Better performances, in terms of rheological and elastomeric properties, were also found for the crude and vulcanized samples prepared by (IndH₄)₂ZrCl₂/MAO catalyst.     

How changes affect software entropy: an empirical study

Context Software systems continuously change for various reasons, such as adding new features, fixing bugs, or refactoring. Changes may either increase the source code complexity and disorganization, or help to reducing it. Aim This paper empirically investigates the relationship of source code complexity and disorganization—measured using source code change entropy—with four factors, namely the presence of refactoring activities, the number of developers working on a source code file, the participation of classes in design patterns, and the different kinds of changes occurring on the system, classified in terms of their topics extracted from commit notes. Method We carried out an exploratory study on an interval of the life-time span of four open source systems, namely ArgoUML, Eclipse-JDT, Mozilla, and Samba, with the aim of analyzing the relationship between the source code change entropy and four factors: refactoring activities, number of contributors for a file, participation of classes in design patterns, and change topics. Results The study shows that (i) the change entropy decreases after refactoring, (ii) files changed by a higher number of developers tend to exhibit a higher change entropy than others, (iii) classes participating in certain design patterns exhibit a higher change entropy than others, and (iv) changes related to different topics exhibit different change entropy, for example bug fixings exhibit a limited change entropy while changes introducing new features exhibit a high change entropy. Conclusions Results provided in this paper indicate that the nature of changes (in particular changes related to refactorings), the software design, and the number of active developers are factors related to change entropy. Our findings contribute to understand the software aging phenomenon and are preliminary to identifying better ways to contrast it.