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A contact layer element for large deformations 总被引:1,自引:0,他引:1
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Dr. Adarshi P. Welegedara Dr. Ansis Maleckis Ruchira Bandara Dr. Mithun C. Mahawaththa Iresha Dilhani Herath Yi Jiun Tan Dr. Angeliki Giannoulis Prof. Daniella Goldfarb Prof. Gottfried Otting Prof. Thomas Huber 《Chembiochem : a European journal of chemical biology》2021,22(8):1480-1486
The selenol group of selenocysteine is much more nucleophilic than the thiol group of cysteine. Selenocysteine residues in proteins thus offer reactive points for rapid post-translational modification. Herein, we show that selenoproteins can be expressed in high yield and purity by cell-free protein synthesis by global substitution of cysteine by selenocysteine. Complete alkylation of solvent-exposed selenocysteine residues was achieved in 10 minutes with 4-chloromethylene dipicolinic acid (4Cl-MDPA) under conditions that left cysteine residues unchanged even after overnight incubation. GdIII−GdIII distances measured by double electron–electron resonance (DEER) experiments of maltose binding protein (MBP) containing two selenocysteine residues tagged with 4Cl-MDPA-GdIII were indistinguishable from GdIII−GdIII distances measured of MBP containing cysteine reacted with 4Br-MDPA tags. 相似文献
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Kazuo Tsubota Stephen C. Pflugfelder Zuguo Liu Christophe Baudouin Hyo Myung Kim Elisabeth M. Messmer Friedrich Kruse Lingyi Liang Jimena Tatiana Carreno-Galeano Maurizio Rolando Norihiko Yokoi Shigeru Kinoshita Reza Dana 《International journal of molecular sciences》2020,21(23)
Over the past decades, the number of patients with dry eye disease (DED) has increased dramatically. The incidence of DED is higher in Asia than in Europe and North America, suggesting the involvement of cultural or racial factors in DED etiology. Although many definitions of DED have been used, discrepancies exist between the various definitions of dry eye disease (DED) used across the globe. This article presents a clinical consensus on the definition of DED, as formulated in four meetings with global DED experts. The proposed new definition is as follows: “Dry eye is a multifactorial disease characterized by a persistently unstable and/or deficient tear film (TF) causing discomfort and/or visual impairment, accompanied by variable degrees of ocular surface epitheliopathy, inflammation and neurosensory abnormalities.” The key criteria for the diagnosis of DED are unstable TF, inflammation, ocular discomfort and visual impairment. This definition also recommends the assessment of ocular surface epitheliopathy and neurosensory abnormalities in each patient with suspected DED. It is easily applicable in clinical practice and should help practitioners diagnose DED consistently. This consensus definition of DED should also help to guide research and clinical trials that, to date, have been hampered by the lack of an established surrogate endpoint. 相似文献
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Mechanical durability of extruded fish feed must be optimized to lower economic losses as well as emission of organic matter to aquatic environments. The glass transition hypothesis for viscoelastic biopolymers is demonstrated and confirmed experimentally to be valid for extruded fish feed pellets. It is proposed and demonstrated that it is important to avoid early glass transition onset, to optimize the obtained mechanical durability. From the proposed glass transition hypothesis, immediate process relevance to the pre-drying transport mechanism is demonstrated. Furthermore, measured mechanical durability is found to range from 1.5 to 5.0% loss, for different combinations of drying parameters. 相似文献