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141.
Lately software based virtual mobile voice over Internet Protocol (VMVoIP) services have become available to end-users which may endanger profit of regular telephone operators from phone calls. In this situation, telephone operators should decide on a proper mechanism for interconnection with such VMVoIP operators which secures mutual benefit of both operators. In this paper, we study both monopolistic and oligopolistic markets of VMVoIP in which a single VMVoIP and several VMVoIP operators available in the market respectively. We model the interaction among a phone operator, VMVoIP operators and end-users as a leader-follower dynamic game and find a sub-game perfect Nash equilibrium (SPNE) for the game. The SPNE shows the phone operator’s best pricing strategy (i.e., wholesale price for VMVoIP operators and retail price for end-users) and its best decision on the interconnection with VMVoIP operators. We further study economic interpretation of the SPNE. We show that in specific end-users’ demands, the phone operator may increase its total profit by allowing the interconnection and taking right price discrimination strategy. 相似文献
142.
We demonstrate the use of the Integrated Volume Method (IVM) to estimate activity coefficients of semi-volatile organic compounds pertinent to ambient/atmospheric aerosols in binary mixtures. We generate binary solution aerosols with different mole fractions of individual components; for each mixture, we measure total change in aerosol volume upon heating from 25 ○ C to 35 ○ C, with the aerosols being at equilibrium in both states. The change in aerosol volume, or in other words, the partitioning between the particle phase and the gas phase, is used to determine activity coefficients of the compounds as a function of their mole fraction in the mixture. We demonstrate this method using the following four model systems. System 1: adipic acid–pimelic acid, which illustrates polar organic–polar organic interactions. Non-ideal behavior was observed with activity coefficients around three at infinite dilution. System 2: adipic acid–dioctyl sebacate, which illustrates polar organic–non-polar organic interactions. The compounds in this experiment did not form a solution. System 3: adipic acid–ammonium sulfate, which illustrates polar organic–inorganic interactions. The compounds in this experiment did not form a solution. System 4: adipic acid–ambient extracts, which illustrates the potential use of the method to study partitioning behavior of individual components in a complex matrix approximating that of real ambient aerosol. The measured activity coefficients of adipic acid were less than unity for the tested range of mixing ratios, indicating suppression of volatility of this compound in ambient organic matrix. 相似文献
143.
Saleh A. Al‐Shamali Baowei Ji Oscar D. Crisalle Haniph A. Latchman 《国际强度与非线性控制杂志
》2005,15(14):619-634
》2005,15(14):619-634
The Nyquist robust sensitivity margin is proposed as a new scalar indicator of robust stability that also provides a meaningful quantitative assessment of the worst sensitivity realized by the uncertain closed loop. After formulating and discussing in detail the underlying optimization problem required for the calculation of the margin, the approach is applied to the characterization of the robust stability of a closed‐loop featuring a linear system with an affine uncertainty structure and a parametric uncertainty set described by a real rectangular polytope. The capabilities of the methodology are illustrated through examples, which include an approach for quantifying alternative robustness margins, such as a parametric stability margin. The computational algorithm is systematic and can be carried out with high numerical precision. Copyright © 2005 John Wiley & Sons, Ltd. 相似文献
144.
Web-based e-commerce software is typically real-time, responsive, and distributed software that must meet high quality requirements.
Moreover, because of its Internet-based operating environment, e-commerce software must deal with concurrency, synchronization,
and non-deterministic issues (such as delays) primarily caused by the heavy usage load and underlying network conditions.
In this paper, we introduce a generic, cost-effective approach to optimize requirements capture (and subsequent development
and testing) with respect to functional coverage and customer perception of quality. This high-yield approach is presented
and illustrated by application to an e-commerce online shopping system and used to explain the principles of use-case requirements
captured in the unified modeling language.
Published online: 25 July 2001 相似文献
145.
We describe a general method for plasmid assembly that uses yeast and extends beyond yeast-specific research applications. This technology exploits the homologous recombination, double-stranded break repair pathway in Saccharomyces cerevisiae to join DNA fragments. Synthetic, double-stranded "recombination linkers" were used to "subclone" a DNA fragment into a plasmid with > 80% efficiency. Quantitative data on the influence of DNA concentration and overlap length on the efficiency of recombination are presented. Using a simple procedure, plasmids were shuttled from yeast into E. coli for subsequent screening and large-scale plasmid preps. This simple method for plasmid construction has several advantages. (i) It bypasses the need for extensive PCR amplification and for purification, modification and/or ligation techniques routinely used for plasmid constructions. (ii) The method does not rely on available restriction sites, thus fragment and vector DNA can be joined within any DNA sequence. This enables the use of multifunctional cloning vectors for protein expression in mammalian cells, other yeast species, E. coli and other expression systems as discussed. (iii) Finally, the technology exploits yeast strains, plasmids and microbial techniques that are inexpensive and readily available. 相似文献
146.
Fifty methicillin-resistant Staphylococcus aureus (MRSA) initial isolates obtained from patients hospitalized in the orthopedic clinic of the Frankfurt University Hospital and 150 methicillin-sensitive Staphylococcus aureus (MSSA) isolates were investigated in this study to determine whether the Slidex Staph-Kit is capable of differentiating between MRSA and MSSA owing to its unique performance characteristics. The Slidex Staph-Kit is a combined latex hemagglutination test designed to detect clumping factor, protein A, and a specific surface immunogen for S. aureus. Clumping factor-positive strains cause erythrocytes sensitized with fibrinogen to hemagglutinate, thereby resulting in visible red clumps. S. aureus strains deficient in clumping factor agglutinate latex particles sensitized with specific antibodies against surface proteins of S. aureus, thereby resulting in visible white clumps. Our results demonstrate that white clumping has a 99% specificity as well as a 98% positive predictive value for MRSA. Clumping factor-negative MRSA, which have been reported to occur in several countries, are epidemic in the Frankfurt area and account for 80% of all MRSA initial isolates in the orthopedic clinic of the Frankfurt University Hospital. Genotyping of all MRSA isolates by macrorestriction analysis of chromosomal DNA revealed that 83% of clumping factor-negative MRSA are closely related to the "southern-German" epidemic strain. This is the first study demonstrating the Slidex Staph-Kit's capability for identifying epidemic clumping factor-negative S. aureus strains as methicillin resistant even prior to antimicrobial susceptibility testing. 相似文献
147.
148.
The effect of dead space on the statistics of the gain in a double-carrier-multiplication avalanche photodiode (APD) is determined using a recurrence method. The dead space is the minimum distance that a newly generated carrier must travel in order to acquire sufficient energy to become capable of causing an impact ionization. Recurrence equations are derived for the first moment, the second moment, and the probability distribution function of two random variables that are related, in a deterministic way, to the random gain of the APD. These equations are solved numerically to produce the mean gain and the excess noise factor. The presence of dead space reduces both the mean gain and the excess noise factor of the device. This may have a beneficial effect on the performance of the detector when used in optical receivers with photon noise and circuit noise 相似文献
149.
Protein kinase C (PKC) acutely increases calcium currents in Aplysia bag cell neurons by recruiting calcium channels different from those constitutively active in the plasma membrane. To study the mechanism of PKC regulation we previously identified two calcium channel alpha1-subunits expressed in bag cell neurons. One of these, BC-alpha1A, is localized to vesicles concentrated primarily in somata and growth cones. We used antibodies to BC-alpha1A to analyze its expression in the bag cell neurons of juvenile Aplysia at a developmental stage at which PKC-sensitive calcium currents have previously been shown to be low. We find that vesicular BC-alpha1A staining is generally reduced in juvenile bag cell neurons but that its expression level can vary among juvenile animals. In 17 bag cell clusters examined, the percentage of neurons that displayed punctate alphaBC-alpha1A staining ranged from 0 to 85%. Sampling of calcium currents from cells of the same clusters by whole cell patch-clamp techniques revealed that the PKC-sensitive calcium current density is significantly correlated with the degree of vesicular staining. In contrast, no correlation of basal calcium current levels with aBC-alpha1A staining was found. These results strongly suggest that BC-alpha1A, a member of the ABE-subfamily of calcium channels, carries the PKC-sensitive calcium current in bag cell neurons. They are consistent with a model in which PKC recruits channels from the vesicular pool to the plasma membrane. 相似文献
150.
Saleh A. H. Khalil Sawsan Abd El-Fattah M. A. Shams-Eldeen 《Drug development and industrial pharmacy》1984,10(10):1737-1755
A study has been carried out to investigate the dissolution rate profiles of twelve batches of sugar-coated phenylbutazone tablets belonging to four commercial brands. Using the rotating basket method, significant inter-brand and inter-batch variations in dissolution rates were found. Only two batches of one brand passed the B.P. dissolution limit whilst other batches had percentages dissolution between 0.3 and 58 after 45 min. Batches with poor dissolution characteristics exhibited significant tablet-to-tablet variations in dissolution rates; a finding which was not observed in the relatively fast-dissolving batches. When the paddle method was substituted for the B.P. basket method, the dissolution rates were relatively faster but similar dissolution failure was found. However, the tablet-to-tablet dissolution variability was decreased in some of the batches. The observed differences in dissolution rates of the batches examined were unrelated to their disintegration times. Inspite of the poor dissolution characteristics of most of the batches studied, no apparent chemical degradation was found. It is recommended that when evaluating the dissolution rates of brands of phenylbutazone tablets, a number of batches from each brand should be tested. 相似文献