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51.

This paper proposes a modular system of precision agriculture to automate sprayers, optimizing the application of pesticides through a robotic system based on computer vision and individual nozzle on/off control. The system uses low-cost equipment such as Arduino boards, solenoid valves, pressure and flow sensors, smartphone, webcam, and Raspberry Pi. The motivation is to reduce the amount of pesticides applied in crops, not just for potential savings for the farmers, but also for environment protection issues, as well as for food safety. The system can be used in any crop planted in rows such as onion, soybean, corn, beans, and rice. The results show that our system can detect lines in plantations and can be used to retrofit conventional boom sprayers, so it is an important step to develop a kit capable of upgrade a conventional sprayer to a fully autonomous robotic sprayer even at affordable cost in the context of small and medium size farms.

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Background. Monitoring of biological TNF inhibitors is a very important tool to guide clinical decisions using specialized algorithms, especially in gastroenterology. A new chemiluminescent instrument (i-TRACK10® from Theradiag) could replace ELISA techniques to calculate the dosage of drugs and anti-drug antibodies. In this bi-centric study, we explored the analytical performances of i-TRACK10® using manual or automated (DS2®) ELISA Lisa-Tracker® assays, and compared the results. Patients and methods. Intra- and inter-run performances were evaluated with i-TRACK10® in two different laboratories and for two different ranges of values for infliximab, adalimumab, and their respective antibodies. Patients’ samples were used in the labs to compare the results obtained between the new instrument and either the manual Lisa-Tracker® or the automated DS2. Results. Intra- and inter-run performances were satisfactory, with values between 1.8% and 16.1% (for inter-run imprecision at low/medium values of infliximab). Results were generally comparable between assays. with the lowest value of correlation at 0.59 (anti-adalimumab dosage between i-TRACK10® and manual ELISA). Most often, values of drugs and anti-drug antibodies were higher with i-TRACK10® than with manual ELISA assay, and correlation values were better with automated ELISA. Agreements were globally acceptable, and the lowest coefficients of 0.7 was obtained for adalimumab values between i-TRACK10® and the two ELISA methods, and for anti-adalimumab values between i-TRACK10® and manual ELISA. The type of assay can potentially induce a change in the class of patients and lead to divergent therapeutic decisions. Conclusions. The new random-access instrument i-TRACK10® presents many advantages in a routine laboratory: rapidity, the possibility of standardization, usability, and expansion of the measurement range. Despite the relatively good agreement of results, it is preferable to use the same assay in longitudinal follow-up of a patient, because quantitative results were not completely equivalent especially for anti-drug antibodies.  相似文献   
54.
Salima Boutti 《Polymer》2005,46(4):1211-1222
A process for the production of high solid content latexes with low viscosity that does not require the use of intermediate seeds is developed. It is shown that the second population of a bimodal latex can be generated by controlled homogeneous nucleation through the use of a combination of an initiator that generates charged free radicals and a mixed surfactant system that is very rich in non-ionic surfactant. The process was shown to be reproducible, and was used to produce a latex with a solid content of almost 77% (v/v) and a viscosity of 1.5 Pa s at a shear rate of 20 s−1.  相似文献   
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Due to different oral and dental conditions, oral mucosa lesions such as those caused by the human papilloma virus and temporomandibular joint pathologies often have to be treated by surgical, ablative or extractive procedures. The treatment and control of pain and inflammation during these procedures is essential to guarantee the patient’s well-being. For the foregoing reason, a hydrogel based on sodium alginate and hyaluronic acid containing 2% of ketorolac tromethamine has been developed. We characterized it physically, mechanically and morphologically. The rheological results suggest that the formulation can be easily and gently applied. Ex vivo permeation studies show that Ketorolac Tromethamine is able to penetrate through the buccal and sublingual mucosae, in addition to being retained in the mucosae’s structure. Through an in vitro test, we were able to evaluate the role that saliva plays in the bioavailability of the drug, observing that more than half of the applied dose is eliminated in an hour. The histological and cytotoxic studies performed on pigs in vivo showed the excellent safety profile of the formulation, as well as its high tolerability. In parallel, a biomimetic artificial membrane (PermeaPad®) was evaluated, and it showed a high degree of correlation with the oral and sublingual mucosa.  相似文献   
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Enterovirus A71 (EV-A71) is a major neurovirulent agent capable of causing severe hand, foot and mouth disease (HFMD) associated with neurological complications and death. Currently, no FDA-approved antiviral is available for the treatment of EV-A71 infections. The flavonoid silymarin was shown to exert virucidal effects, but the binding site on the capsid was unknown. In this study, the ligand interacting site of silymarin was determined in silico and validated in vitro. Moreover, the potential of EV-A71 to develop resistance against silymarin was further evaluated. Molecular docking of silymarin with the capsid of EV-A71 indicated that silymarin binds to viral protein 1 (VP1) of EV-A71, specifically at the GH loop of VP1. The in vitro binding of silymarin with VP1 of EV-A71 was validated using recombinant VP1 through ELISA competitive binding assay. Continuous passaging of EV-A71 in the presence of silymarin resulted in the emergence of a mutant carrying a substitution of isoleucine by threonine (I97T) at position 97 of the BC loop of EV-A71. The mutation was speculated to overcome the inhibitory effects of silymarin. This study provides functional insights into the underlying mechanism of EV-A71 inhibition by silymarin, but warrants further in vivo evaluation before being developed as a potential therapeutic agent.  相似文献   
57.
The purpose of semantic web services discovery is to use semantic knowledge to find services that can be composed to form an admissible answer to a query. In this paper we consider the advantage of adopting a knowledge-based approach to web services discovery. Services are described by means of terminological axioms and constrained clauses. We apply a specific resolution and a constraint propagation mechanism to evaluate queries over services.Recommended by: Athman Bouguettaya and Boualem Benatallah  相似文献   
58.
Organisations are increasingly relying on Big Data to provide the opportunities to discover correlations and patterns in data that would have previously remained hidden, and to subsequently use this new information to increase the quality of their business activities. In this paper we present a ‘story’ of Big Data from the initial data collection and to the end visualization, passing by the data fusion, and the analysis and clustering tasks. For this, we present a complete work flow on (a) how to represent the heterogeneous collected data using the high performance RDF language, how to perform the fusion of the Big Data in RDF by resolving the issue of entity disambiguity and how to query those data to provide more relevant and complete knowledge and (b) as the data are received in data streams, we propose batchStream, a Micro-Batching version of the growing neural gas approach, which is capable of clustering data streams with a single pass over the data. The batchStream algorithm allows us to discover clusters of arbitrary shapes without any assumptions on the number of clusters. This Big Data work flow is implemented in the Spark platform and we demonstrate it on synthetic and real data.  相似文献   
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