Comparison of filter media,plant communities and microbiology within constructed wetlands treating wastewater containing heavy metals

The aim of this study was to investigate the treatment efficiency of passive vertical‐flow wetland filters containing Phragmites australis and/or Typha latifolia and granular media of different adsorption capacities. A cost–benefit analysis was performed on the characteristics of constructed wetlands. Cheap gravel and sand as well as expensive granular activated carbon, charcoal and Filtralite (light expanded clay) were used as filter media. Different concentrations of lead and copper sulfate were added to polluted urban beck inflow water in order to simulate pre‐treated mine wastewater, landfill leachate or highway runoff. The relationships between growth media, microbial and plant communities as well as the reduction of lead, copper, five‐day biochemical oxygen demand (BOD) in particular, and potentially pathogenic bacteria were investigated. A breakthrough of copper within the first 9 weeks was only recorded for filters containing media with limited adsorption capacities. The breakthrough was independent of the presence of plants. However, after maturation of the biomass, which dominated the schmutzdecke and litter zone, lead and copper concentrations were reduced by 97–99% and BOD by 41–60% in all wetlands. There appears to be no additional benefit in using macrophytes and expensive adsorption media in constructed wetlands to enhance metal reduction during the set‐up period of 5 months. © 2001 Society of Chemical Industry     

Ecological equilibrium on biological activated carbon

This paper examines the potential of a biological activity control system (BACS) for biological activated carbon (BAC) in comparison to granular activated carbon (GAC) for the treatment of potable water. The overall objective of the project is to produce drinking water of a higher quality more economically by developing a BACS for exhausted GAC that can be transformed to BAC by the development of a natural biofilm during the bio-regeneration mode. The research therefore may be interesting for water companies and the activated carbon industry. Findings show that the lifetime of a GAC filter can be significantly extended by maintaining an active biofilm that has to be controlled in order to avoid filter clogging. The most important parameters are dissolved oxygen (DO), pH and a correct balance of nutrients, which enables a natural control of the biomass. pH control was required to maintain an optimal bacteria-protozoa level. Excessive growth of filamentous bacteria can be prevented by a decrease in DO, increase in pH and the reduction of one essential nutrient, e.g. total phosphorus (P). Total organic carbon (TOC) and chemical oxygen demand (COD) values were reduced by bioactivity. DO, turbidity and suspended solids (SS) values were kept in acceptable ranges with respect to drinking water objectives. Plants without a significant population of protozoa deliver turbid low quality effluent high on SS and biochemical oxygen demand (BOD). It was possible to control the biofilm on GAC containing a natural biofilm and BAC during the bio-regeneration mode. Natural and artificial bio-regeneration lead to similar performance characteristics.     

Effects of the Na+/H+-exchange inhibitor Hoe 642 on intracellular pH, calcium and sodium in isolated rat ventricular myocytes

The inhibitors of the Na+/H+-exchange (NHE1) system Hoe 694 and Hoe 642 possess cardioprotective effects in ischaemia/reperfusion. It is assumed that these effects are due to the prevention of intracellular sodium (Nai) and calcium (Cai) overload. The purpose of the present study was to investigate the effects of Hoe 642 on intracellular pH, Na+ and Ca2+ (pHi, Nai and Cai) in isolated rat ventricular myocytes under anoxic conditions or in cells in which oxidative phosphorylation had been inhibited by 1.5 mmol/l cyanide. In cells which were dually loaded with the fluorescent dyes 2, 7-biscarboxyethyl-5,6-carboxyfluorescein (BCECF) and Fura-2, anoxia caused acidification of the cells (from pHi 7.2 to pHi 6.8) and an increase in Cai from about 50 nmol/l to about 1 micromol/l. The decrease in pHi began before the cells underwent hypoxic (rigor) contracture, whereas Cai only began to rise after rigor shortening had taken place. After reoxygenation, pHi returned to its control value and Cai oscillated and then declined to resting levels. It was during this phase that the cells rounded up (hypercontracture). When 10 micromol/l Hoe 642 was present from the beginning of the experiment, pHi and Cai were not significantly different from control experiments. At reoxygenation, pHi did not recover, but Cai oscillated and returned to its resting level. To monitor Nai, the cells were loaded with the dye SBFI. After adding 1.5 mmol/l cyanide or 100 micromol/l ouabain, Nai increased from the initial 8 mmol/l to approximately 16 mmol/l. Hoe 642 or Hoe 694 (10 micromol/l) did not prevent the increase in Nai. In contrast, the blocker of the persistent Na+ current R56865 (10 micromol/l) attenuated the CN--induced rise in Nai. The substance ethylisopropylamiloride was not used because it augmented considerably the intensity of the 380 nm wavelength of the cell's autofluorescence. In conclusion, the specific NHE1 inhibitor Hoe 642 did not attenuate anoxia-induced Cai overload, nor CN--induced Nai and Cai overload. Hoe 642 prevented the recovery of pHi from anoxic acidification. This low pHi maintained after reoxygenation may be cardioprotective. Other possible mechanisms of NHE1 inhibitors, such as prevention of Ca2+ overload in mitochondria, cannot be ruled out. The increase in Nai during anoxia is possibly due to an influx of Na+ via persistent Na+ channels.     

Oxide ceramic fibers via dry spinning process—From lab to fab

Oxide fibers preparation and manufacturing capabilities at Fraunhofer-Center HTL are introduced, showing the development and preparation of oxide ceramic fibers from lab scale to pilot scale up to near production scale. As a specific example, the development of an aluminosilicate fiber with mullite composition is discussed in more detail. Fiber development started from nonaqueous sol-gel precursors in the early lab scale. With increasing fiber spinning volume, precursors were switched to water-soluble systems. Transformation from green fiber to ceramic fiber was monitored by thermogravimetric and differential thermal analysis, X-ray diffraction, and scanning electron microscopy. The evolution of ceramic phases, microstructure formation, and the effects on tensile strength and Young's modulus were investigated. Weibull statistics and fracture analysis helped to understand the results. Next step will be the transition from large lab scale to pilot scale, demonstrating manufacturing capability.     

Order barriers for the B-convergence of ROW methods

The convergence of ROW methods is studied when these methods are applied to the stiff model equation of Prothero and Robinson. It turns out that there are barriers of the attainable order of convergence. Furthermore, the existence of ROW methods is shown the accuracy of which increases asymptotically with the stiffness of the model. The theoretical results are demonstrated by numerical examples.     

Cyclophilin and trigger factor from Bacillus subtilis catalyze in vitro protein folding and are necessary for viability under starvation conditions

Cyclophilin (the product of the ppiB gene) and the trigger factor (the product of the tig gene) are the only cytosolic peptidyl-prolyl cis-trans isomerases that are known in Bacillus subtilis. Both enzymes catalyze the in vitro refolding of ribonuclease T1, a reaction that is limited in rate by a prolyl cis/trans isomerization. The efficiency of cyclophilin as a folding catalyst is only modest with a kcat/KM value of 3.8 x 10(4) M-1 s-1, but the trigger factor shows an almost 40-fold higher specific activity with a kcat/KM value of 1.4 x 10(6) M-1 s-1. This high catalytic activity originates from the tight binding to the protein substrate as reflected in both the low KM value of 0.5 microM and in the strong inhibition of the trigger factor by unfolded proteins. By use of a protein-folding assay, the concentrations of cyclophilin and the trigger factor in the cytosol of B. subtilis could be determined as 26 and 35 microM, respectively. Together they account for the entire folding activity that is detectable in crude extracts of wild-type B. subtilis cells. The genes encoding cyclophilin and the trigger factor in the B. subtilis chromosome were disrupted individually and simultaneously. Even in combination, these disruptions had no effect on cell viability in rich medium or under several stress conditions, such as heat, osmotic, or oxidative stress. However, in poor medium and, in particular, in the absence of amino acids, the growth of the double mutant strain was strongly decelerated, indicating that the prolyl isomerases become essential for growth under starvation conditions. It is not yet known whether this function relates to the catalysis of the proline-limited folding of essential proteins.     

Inactivation of (Gialpha) proteins increases arrhythmogenic effects of beta-adrenergic stimulation in the heart

Chronic treatment of rats with carbachol downregulates M-cholinoceptors and inhibitory, pertussis toxin (PTX)-sensitive G proteinalpha-subunits (Gialpha) and sensitizes the heart to arrhythmogenic effects of isoprenaline (ISO), suggesting a causal relationship. To test this hypothesis by a more direct and quantitative approach, nine groups of rats were treated for 24 h with increasing doses of PTX (1.25-200 microg/kg i.v.). Inactivation of cardiac Gialpha was determined biochemically by 32P-ADP-ribosylationin vitro and functionally by measuring contractile effects of carbachol. Effects of ISO were studied in spontaneously beating right atria (RA) and isolated papillary muscles (PM; paced at 1 Hz). PTX increased heart rate in conscious animals (ECG) with a bell-shaped dose-dependency (maximal increase 120 beats/min at 7.5 microg/kg). PTX dose-dependently inactivated 25-85% of total cardiac Gialpha, which linearly correlated with a loss of the direct negative chronotropic effect of carbachol in atria, but not with a loss of its indirect negative inotropic effect in PM. The latter was resistant up to PTX 20 microg/kg (=70% inactivation). The decrease in Gialpha closely correlated with an increased efficacy of ISO to induce spontaneous contractile activity (automaticity) in PM. At 3 micromol/l ISO, all PM from PTX 200 microg/kg beat spontaneously compared to 10% in control. In contrast, pretreatment with PTX only modestly and not clearly dose-dependently increased the inotropic potency of ISO (PTX 100 microg/kg: EC50 28v 81 nmol/l in control) and did not affect the chronotropic effect of ISO. The disparity of the functional consequences of PTX treatment suggest that under physiological conditions, Gialpha serve mainly to suppress arrhythmogenic, but not or to a minor extent, positive chronotropic or inotropic effects of beta-adrenoceptor activation.     

Electrochemical investigations on the stationary and non-stationary behaviour of the PbO2 electrode

The kinetic behaviour of the PbO₂ electrode is determined by three reactions: (i) deposition/dissolution of lead dioxide, (ii) changes in the stoichiometry of the oxide, especially by incorporation/dissolution of hydrogen; (iii) anodic evolution of oxygen.Immediately following commencement of current flow the stoichiometry-changing reaction predominates, because, we postulate, the proton transfer across the PbO₂/electrolyte phase boundary is almost uninhibited. Investigations of the kinetics of this reaction have therefore been carried out by means of current pulse measurements. Under appropriate conditions the current in the stationary state is used for PbO₂ deposition/dissolution only. Current-overvoltage curves for these reactions have been obtained from measurements with thin PbO₂ layers, where the stationary state was reached quickly.