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991.
The interactions of the 936-species phages sk1, jj50, and 64 with the cell surface of Lactococcus lactis LM0230 were analyzed. Cell envelopes (walls + plasma membrane), cell wall, or plasma membrane from L. lactis ssp. lactis LM0230 each inactivated the phages in vitro. However, other 936-species phages kh and P008, which do not infect strain LM0230, were not inactivated by any of the subcellular fractions. Treating cell walls or plasma membrane with the cell wall hydrolase mutanolysin eliminated inactivation of phage sk1. This suggested that intact cell wall fragments were required for inactivation. A role for plasma membrane in phage sk1 inactivation was further investigated. Boiling, washing in 2 M KCl, 8 M urea, or 0.1 M Na(2)CO(3)/pH 11, or treating the plasma membrane with proteases did not reduce adsorption or inactivation of phage. Adding lipoteichoic acid or antibodies to lipoteichoic acid did not reduce inactivation of phage in a mixture with membrane, suggesting that lipoteichoic acid was not involved. Inactivation by envelopes or cell wall correlated with ejection of DNA from the phage sk1 capsid. Although calcium is required for plaque formation, it was not required for adsorption, inactivation, or ejection of phage DNA by envelopes or cell wall. The results suggest that at least for phages sk1, jj50, and 64, adsorption and phage DNA injection into the host does not require a host membrane protein or lipoteichoic acid, and that cell wall components are sufficient for these initial steps of phage infection. 相似文献
992.
超低碳奥氏体不锈钢00Cr18Ni10还原脱磷研究 总被引:1,自引:0,他引:1
在 2 5kg真空感应炉上用镁钙合金对超低碳奥氏体不锈钢 0 0Cr18Ni10进行了还原脱磷试验。通过充入工业纯氩使炉内压力为 6 6 .6 6kPa和精炼时的反应温度 14 5 0~ 15 0 0℃ ,加入镁钙合金 5 0kg t和助熔剂CaF2 11kg t,可使精炼后 0 0Cr18Ni10的磷含量进一步降低到 (5 0~ 6 0 )× 10 - 6 ,钢中氧、硫含量分别稳定降到(4~ 6 )× 10 - 6 和 15× 10 - 6 。 相似文献
993.
994.
995.
To study the effect of ischemia reperfusion injury on microvascular reactivity and tissue metabolism in skeletal muscle, a Sprague-Dawley rat cremaster muscle was prepared as a tourniquet ischemia model and subjected to 2 hr ischemia followed by 1 hr reperfusion to simulate the timing of ischemia during microvascular surgery. The dose-response curve of arteriolar reactivity to norepinephrine, lipid peroxidation, and ultrastructure of capillaries was determined in both the control and postischemic reperfusion stages. Judging from the results, we summarize our observations as follows: (1) Postischemic reperfusion significantly increased arteriolar reactivity to norepinephrine, in which the EC50 for vasoconstriction decreased in all three orders of arterioles. These results suggest that reperfusion could have impaired the vasodilation control mechanism, possibly being endothelium dependent. (2) Lipid peroxidation increased sixfold in the reperfusion group, suggesting that oxygen free radicals have produced significant tissue damage under the created conditions. (3) Significant endothelial damage in the capillaries shown by electron microscope observation supports these studies, indicating that ischemia/reperfusion in clinically transplanted skeletal muscles could cause significant damage to the tissue microcirculation both physiologically and metabolically. 相似文献
996.
钼还原过程相变化研究 总被引:5,自引:0,他引:5
采用钼酸铵直接氢还原及钼酸铵先焙解然后再分段氢还原两种不同的还原方法进行了钼粉还原。通过X射线衍射方法分析了两种工艺还原过程中的反应序列及相变化规律,并讨论了不稳定相Mo4O11对于钼粉还原过程的影响。 相似文献
997.
将数字样机技术应用到某型号飞机的前起落架机构的可靠性设计分析中,成功解决了其中的关键技术,取得良好的效果.以工程应用为目的,在商业软件基础上进行了二次开发,搭建了一套基于数字样机技术的机构可靠性设计分析系统,同时引入了灵敏度分析,将可靠性设计与灵敏度分析相结合,提高了可靠性设计分析的效率,增强了该技术的工程实用性. 相似文献
998.
999.
Preparation of Neodymium-Doped Yttrium Aluminum Garnet Transparent Ceramics by Homogeneous Precipitation Method 总被引:3,自引:0,他引:3
Neodymium-doped yttriumaluminumgarnet(Nd∶YAG)transparent polycrystalline ceramics,laser ce-ramic matrix materials,are gradually becomingsubsti-tutes for single crystals because they are provided withpredominantly chemical stability,highly doped ionconcentration,excellent optical performance and hightemperature stability[1,2].Presently,the conventionallyused methods to produce Nd∶YAG powders are solid-state processes[3],chemical precipitation methods[4,5]andlowtemperature combustiontechniqu… 相似文献
1000.