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191.
Clment Leveque Simona Mrakic-Sposta Pierre Lafre Alessandra Vezzoli Peter Germonpr Alexandre Beer Stphane Mievis Fabio Virgili Kate Lambrechts Sigrid Theunissen Franois Guerrero Costantino Balestra 《International journal of molecular sciences》2023,24(1)
Oxygen is a powerful trigger for cellular reactions and is used in many pathologies, including oxidative stress. However, the effects of oxygen over time and at different partial pressures remain poorly understood. In this study, the metabolic responses of normobaric oxygen intake for 1 h to mild (30%) and high (100%) inspired fractions were investigated. Fourteen healthy non-smoking subjects (7 males and 7 females; age: 29.9 ± 11.1 years, height: 168.2 ± 9.37 cm; weight: 64.4 ± 12.3 kg; BMI: 22.7 ± 4.1) were randomly assigned in the two groups. Blood samples were taken before the intake at 30 min, 2 h, 8 h, 24 h, and 48 h after the single oxygen exposure. The level of oxidation was evaluated by the rate of reactive oxygen species (ROS) and the levels of isoprostane. Antioxidant reactions were observed by total antioxidant capacity (TAC), superoxide dismutase (SOD), and catalase (CAT). The inflammatory response was measured using interleukin-6 (IL-6), neopterin, creatinine, and urates. Oxidation markers increased from 30 min on to reach a peak at 8 h. From 8 h post intake, the markers of inflammation took over, and more significantly with 100% than with 30%. This study suggests a biphasic response over time characterized by an initial “permissive oxidation” followed by increased inflammation. The antioxidant protection system seems not to be the leading actor in the first place. The kinetics of enzymatic reactions need to be better studied to establish therapeutic, training, or rehabilitation protocols aiming at a more targeted use of oxygen. 相似文献
192.
Internet of Construction: Potential of LoRaWAN quality assurance in the In-situ concrete prozess Achieving high quality assurance standards when using in-situ concrete requires a great amount of manual documentation. In particular, in-situ concrete installation requires a great deal of attention, as the use of the right concrete in the intended building element must be ensured and errors mean additional time and costs. In other industries, it can be observed that IoT systems are already used profitably to support the execution processes and for documentation needs. However, despite the numerous digital solutions available, the use of Internet of Things (Iot) technologies for such monitoring tasks on the construction site are rarely observed. Yet the technologies have the potential to simplify quality assurance measures and make construction more attractive. This paper deals with the process step of concrete installation within the value chain of in-situ concrete construction, which has so far been performed mainly manually, involves a high risk of error and is very time-consuming to monitor. A Long Range Wide Area Network (LoRaWAN-)-based IoT system is designed to support humans in documenting the concrete installations. The system will then undergo a field test on a construction site. The results obtained are compared with the process-related requirements, and with this it is shown that some important requirements are already satisfactorily fulfilled with the presented setup. 相似文献
193.
Stephanie Arndt Fadi Fadil Katja Dettmer Petra Unger Marko Boskovic Claudia Samol Anja-Katrin Bosserhoff Julia L. Zimmermann Michael Gruber Wolfram Gronwald Sigrid Karrer 《International journal of molecular sciences》2021,22(15)
Cold Atmospheric Plasma (CAP) is an ionized gas near room temperature. Its anti-tumor effect can be transmitted either by direct treatment or mediated by a plasma-treated solution (PTS), such as treated standard cell culture medium, which contains different amino acids, inorganic salts, vitamins and other substances. Despite extensive research, the active components in PTS and its molecular or cellular mechanisms are not yet fully understood. The purpose of this study was the measurement of the reactive species in PTS and their effect on tumor cells using different plasma modes and treatment durations. The PTS analysis yielded mode- and dose-dependent differences in the production of reactive oxygen and nitrogen species (RONS), and in the decomposition and modification of the amino acids Tyrosine (Tyr) and Tryptophan (Trp). The Trp metabolites Formylkynurenine (FKyn) and Kynurenine (Kyn) were produced in PTS with the 4 kHz (oxygen) mode, inducing apoptosis in Mel Im melanoma cells. Nitrated derivatives of Trp and Tyr were formed in the 8 kHz (nitrogen) mode, elevating the p16 mRNA expression and senescence-associated ß-Galactosidase staining. In conclusion, the plasma mode has a strong impact on the composition of the active components in PTS and affects its anti-tumor mechanism. These findings are of decisive importance for the development of plasma devices and the effectiveness of tumor treatment. 相似文献
194.
195.
The mechanical properties and the swelling behaviour of aqueous poly(acryl amide) and poly(acryl amide-co-Na acrylate) gels were investigated. The ionic networks were prepared by hydrolysis of the nonionic ones, in this way preserving the network topology. By measurement of the modulus it was detected that networks prepared in solution contain a rather large portion of elastically ineffective rings. Hydrolysing a part of the amide groups resulted in a considerably increased swelling ratio. The modulus of such ionic gels did not show the predicted decrease with rising swelling ratio, but on the contrary increased up to a fivefold value. The reason for this behaviour is seen in the limited extensibility (non-Gaussian behaviour) of the network chains. At constant swelling ratio, the modulus of gels having identical topology decreased with rising degree of hydrolysis. This reflects the influence of the ionic groups on the mean-square end-to-end distance of the free polymer chains. 相似文献
196.
Elena Bartkiene Vadims Bartkevics Iveta Pugajeva Vita Krungleviciute Sigrid Mayrhofer Konrad Domig 《International Journal of Food Science & Technology》2017,52(6):1473-1482
A Lactobacillus plantarum strain was used for the production of rye, wheat, barley, and oat sourdoughs, and the influence of different sourdoughs on mixed rye–wheat bread quality parameters and acrylamide formation was evaluated. L. plantarum LUHS135 demonstrated versatile carbohydrate metabolism, good growth and acidification rates, and the ability to excrete amylolytic and proteolytic enzymes in various cereal sourdoughs. The same starter and different cereal substrates allow to produce sourdoughs showing different characteristics. The type of sourdough and its quantity had significant influence on acrylamide content in bread (P ≤ 0.0001), and using 5% or 10% of wheat sourdough, 5%, 15%, or 20% of barley sourdough, and 5% or 15% of oat sourdough, it is possible to reduce acrylamide content in bread. Thus, manufacturers need to take into account application of apparent technological approaches for acrylamide in bread reducing. 相似文献
197.
In the paper, an integrated approach for the modeling and enforcement of delegation policies in process-aware information systems is presented. In particular, a delegation extension for process-related role-based access control (RBAC) models is specified. The extension is generic in the sense that it can be used to extend process-aware information systems or process modeling languages with support for process-related RBAC delegation models. Moreover, the detection of delegation-related conflicts is discussed and a set of pre-defined resolution strategies for each potential conflict is provided. Thereby, the design-time and runtime consistency of corresponding RBAC delegation models can be ensured. Based on a formal metamodel, UML2 modeling support for the delegation of roles, tasks, and duties is provided. A corresponding case study evaluates the practical applicability of the approach with real-world business processes. Moreover, the approach is implemented as an extension to the BusinessActivity library and runtime engine. 相似文献
198.
Andrea Kellenberger Nicolae Vaszilcsin Mircea Laurentiu Dan Ion Mitelea Alexandru Adrian Geana Sigrid Lædre Corneliu M. Craciunescu 《金属学报(英文版)》2024,37(4):607
The components of proton exchange membrane water electrolysers frequently experience corrosion issues, especially at high anodic polarization, that restrict the use of more affordable alternatives to titanium. Here, we investigate localized corrosion processes of bare and Ti-coated AISI 446 ferritic stainless steel under anodic polarization by scanning electrochemical microscopy (SECM) in sodium sulphate and potassium chloride solutions. SECM approach curves and area scans measured at open-circuit potential (OCP) of the samples in the feedback mode using a redox mediator evidence a negative feedback effect caused by the surface passive film. For the anodic polarization of the sample, the substrate generation-tip collection mode enables to observe local generation of iron (II) ions, as well as formation of molecular oxygen. For the uncoated AISI 446 sample, localized corrosion is detected in sodium sulphate solution simultaneously with oxygen formation at anodic potentials of 1.0 V vs. Ag/AgCl, whereas significant pitting corrosion is observed even at 0.2 V vs. Ag/AgCl in potassium chloride solution. The Ti-coated AISI 446 sample reveals enhanced corrosion resistance in both test solutions, without any evidence of iron (II) ions generation at anodic potentials of 1.2 V vs. Ag/AgCl, where only oxygen formation is observed. 相似文献