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61.
Mineralocorticoid action is facilitated by 11 beta-hydroxysteroid dehydrogenase type II (11 beta HSD2), which metabolizes glucocorticoids and allows aldosterone to bind to the nonselective mineralocorticoid receptor. We have recently demonstrated the presence of the 11 beta HSD2 protein in a wide range of human epithelia, suggesting that it is the sole isoform endowing specificity in man. In the present study we have used an immunopurified polyclonal antibody (RAH23) raised against a C-terminal peptide derived from the cloned rat 11 beta HSD2 protein to perform immunohistochemical and molecular analysis in rat tissues. In frozen sections of rat kidney, strong staining was seen with the RAH23 antibody in the distal tubule; weaker staining was observed in the thick ascending loop of Henle and the medullary and papillary collecting ducts. Punctate cortical staining was observed in the fetus at 20 days gestation and in 8-day-old rats, with a noticeable increase in the staining pattern at 16 days of age. The kidney did not attain the adult pattern of staining until 28 days of age. Epithelia of ileum and colon also stained with RAH23, as did excretory ducts of the submandibular gland. Intrahepatic and excretory bile ducts displayed strong immunoreactivity in the epithelial lining. Rat adrenal glands showed evidence of the 11 beta HSD2 antigen in the zona fasciculata and zona reticularis, but not in the zona glomerulosa or medulla. Western blot analysis with the RAH23 antibody revealed strong bands in the kidney, colon, adrenal gland, and submandibular gland at 40 kDa, colinear with the migration of the cloned 11 beta HSD2 enzyme. A band of medium intensity was also seen at this size in the pancreas, whereas a band of moderate intensity was seen in the bile duct, and weaker bands were noticed in the stomach, small intestine, and liver, with a diffuse band at 36-42 kDa in the prostate. Strong bands were seen in the pancreas and prostate at 78 kDa, with weaker signals in the colon, adrenal, stomach, and bile duct. A number of tissues also displayed multiple bands at about 30 kDa. Enzymatic assays on tissue homogenates showed extensive conversion of corticosterone to its 11-dehydro product in an NAD-dependent manner in the submandibular gland, adrenal gland, and kidney, but not in the pancreas or prostate. This study confirms the ubiquitous presence of 11 beta HSD2 in sodium-transporting epithelia, demonstrates the high level of 11 beta HSD2 protein and enzyme activity in the rat adrenal, and suggests a possible role for the enzyme in the biliary system. Further studies are required to determine the relevance of the various molecular species to the activity, latency, and processing of the enzyme. 相似文献
62.
Molybdenum black solar selective coatings have been produced on cobalt by immersion in a solution of ammonium paramolybdate
and nickel sulphate. The cobalt was electroplated on nickel-plated copper prior to immersion in the paramolybdate solution.
The maximum solar absorptance of the resulting molybdenum black coating was about 0.91. The minimum emittance was about 0.1
for coatings on cobalt deposited with an addition agent and about 0.23 for coatings on cobalt deposited without addition agent
in the 60 °C plating solution. These differences have been related to the coating morphology determined by scanning electron
microscopy. The emittance of coatings on cobalt deposited without addition agent decreases (or remains unchanged) during short-term
heat treatment while that of coatings deposited on cobalt plated with addition agent increases somewhat. Reduction of the
cobalt plating bath temperature to 45 °C can also lead to good initial coating properties but without the requirement for
an addition agent in the cobalt plating bath. XPS studies show that the oxidation state of molybdenum in the coatings is approximately
+ 5 corresponding to Mo4O11. This reduces to + 4 after argon ion bombardment. Some cobalt may be present in the coatings in the form of CoO. 相似文献
63.
MR Smith 《Canadian Metallurgical Quarterly》1997,176(6):1374-1375
Colposacropexy procedures restore anatomically correct apical vaginal support on the levator plate at the ischial spine level. Venous hemorrhage resulting from laceration of presacral veins during suture fixation is the major hazard of this procedure. Titanium orthopedic bone anchor fixation minimizes this risk through precision placement of the bone anchor-suture unit. 相似文献
64.
R. Smith 《Journal of Engineering Mathematics》1995,29(6):497-516
A two-dimensional horizontal dispersion equation is derived for the many ways that buoyancy modifies the spreading of a mono-disperse discharge of particles in a shallow-water flow. The particle-induced lateral density gradient gives rise to a secondary transverse flow which tends to carry particles outwards. Stratification with the consequent reduced mixing, changes the local vertical profile of the original flow and gives rise to a Burgers nonlinearity (slowing down of sinking particles or a speeding up of buoyant particles). Stratification also modifies the non-local horizontal distribution of the current, with an inflow towards particle-laden regions where the drag is reduced. A simple eddy-diffusivity turbulence model is used which permits explicit evaluation of most of the linear and nonlinear coefficients. Nonlinear effects are shown to be significant for particle volume concentrations of only 10 parts per million. 相似文献
65.
66.
S. H. Smith 《Journal of Engineering Mathematics》1995,29(2):195-202
The second order boundary layer effects are considered for the classical problem of the impulsive motion past a flat plate. Although Stewartson [1], [2] showed clearly how the effect of the leading edge requires a finite time to enter the boundary layer when only first order effects are included, we see here how this second order contribution is noticed immediately throughout the layer, and so becomes the dominant contribution after the Rayleigh behaviour during this time. 相似文献
67.
We propose a composite multivariate quality control (CMQC) system to control simultaneously measured variables. This system is designed to detect unacceptable trends and systematic error in one or more variables, unacceptable random error in one or more variables, and unacceptable changes in the correlation structure in any pair of variables. It is also designed to be tolerant of missing data, to be capable of rejecting as few as one or as many as all variables in a run, and to provide the analyst with control statistics and graphics that logically relate to sources of analytical error. Quality control rules for univariate, multivariate, and correlation conditions are incorporated in the system, as are plots displaying CMQC statistic values and control limits for univariate, multivariate, and correlation parameters. We also discuss advantages of the CMQC over the T2 and principal component multivariate quality control methods. We demonstrate the CMQC procedure using data from a laboratory process in which 40 variables were measured during 40 characterization runs and 23 runs analyzing unknowns. 相似文献
68.
Ken Smith 《Color research and application》2007,32(4):249-255
This study describes a process of relating the perceptual analysis of the colors of the terrestrial atmosphere to currently available pigments used in artists' painting systems. This process sought to discover how the colors of the sky could be defined and simulated by these pigments. The author also describes how confusion over the bewildering choice of suitable pigments on offer in the market place can be clarified. © 2006 Wiley Periodicals, Inc. Col Res Appl, 32, 249–255, 2007 相似文献
69.
Becker D. Vaughn; Kenrick Douglas T.; Neuberg Steven L.; Blackwell K. C.; Smith Dylan M. 《Canadian Metallurgical Quarterly》2007,92(2):179
Findings of 7 studies suggested that decisions about the sex of a face and the emotional expressions of anger or happiness are not independent: Participants were faster and more accurate at detecting angry expressions on male faces and at detecting happy expressions on female faces. These findings were robust across different stimulus sets and judgment tasks and indicated bottom-up perceptual processes rather than just top-down conceptually driven ones. Results from additional studies in which neutrally expressive faces were used suggested that the connections between masculine features and angry expressions and between feminine features and happy expressions might be a property of the sexual dimorphism of the face itself and not merely a result of gender stereotypes biasing the perception. (PsycINFO Database Record (c) 2011 APA, all rights reserved) 相似文献
70.
Enteric viral infections of calves and passive immunity 总被引:6,自引:0,他引:6
At least eight viruses have been identified, four within the last 5 yr, that produce diarrhea and pathological intestinal lesions in experimentally inoculated calves. Coronavirus and rotavirus frequently are associated with the neonatal calf diarrhea syndrome, but the etiologic role of the newly identified viruses is undefined. All diarrheal viruses replicate within small intestinal epithelial cells, resulting in variable degrees of villous atrophy. Immunity against these viral infections, therefore, must be directed toward protection of the susceptible intestinal epithelial cells. Because most of these viral infections occur in calves less than 3 wk of age, passive lactogenic immunity within the gut lumen plays an important role in protection. This report reviews methods of boosting rotavirus antibody responses in bovine mammary secretions and analyses of passive and active immunity in calves supplemented with colostrum and challenged by rotavirus. Results indicate rotavirus immunoglobulin G1 antibodies in colostrum and milk were elevated after intramuscular and intramammary vaccination of pregnant cows with an Ohio Agricultural Research and Development Center rotavirus vaccine but not after intramuscular immunization with a commercial rota-coronavirus vaccine. Feeding colostrum from intramuscular plus intramammary immunized cows to newborn calves challenged by rotavirus prevented diarrhea and shedding of rotavirus. 相似文献