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151.
Ten site-specific mutants of pea apophytochrome A were expressed in Saccharomyces cerevisiae and analyzed for chromophore assembly with apoprotein and photoreversible absorbance changes. The mutants constitute two specific changes for each of five conserved amino acid residues located in the microenvironment of the chromophore attachment residue, which is Cys-323 in pea phytochrome A. All mutant apophytochromes were autocatalytically able to covalently attach phycocyanobilin, indicating that there were no major structural perturbations in the apoproteins. However, the rate of chromophore ligation varied significantly among the mutants. Spectrally, the mutant holophytochromes are of three types: mutant phytochromes that are indistinguishable from the wild-type adduct, mutants with blue-shifted Pr and Pfr absorption maxima compared to the wild-type adduct, and mutants that are not photoreversible. From an analysis of the results, we concluded that the residues Asp-309, Arg-318, His-321, and Gln-326 are probably not catalytically involved in the chromophore ligation reaction, but some residues may play significant structural and stereochemical roles. Arg-318 might anchor the chromophore, as has been suggested [Partis, M. D., & Grimm, R. (1990) Z. Naturforsch, 45c, 987-998; Parker, W., et al. (1993) Bioconjugate Chem. (in press)]. The conserved Gln-326, three residues downstream from the chromophore attachment site, is not electrostatically critical for the spectral integrity and photoreversibility of phytochrome, but this residue is sterically important to the lyase activity. It appears that the role of the five amino acid residues in the N- and C-terminal vicinities of the chromophore binding Cys-323 is structural rather than catalytic for the ligation reaction. 相似文献
152.
Exhaled air condensate provides a noninvasive means of obtaining samples from the lower respiratory tract. Hydrogen peroxide (H2O2) in exhaled air has been proposed as a marker of airway inflammation. We hypothesized that in stable asthmatic children the H2O2 concentration in exhaled air condensate may be elevated as a result of airway inflammation. In a cross-sectional study, 66 allergic asthmatic children (of whom, 41 were treated with inhaled steroids) and 21 healthy controls exhaled through a cold trap. The resulting condensate was examined fluorimetrically for the presence of H2O2. All subjects were clinically stable, nonsmokers, without infection. The median H2O2 level in the exhaled air condensate of the asthmatic patients was significantly higher than in healthy controls (0.60 and 0.15 micromol, respectively; p<0.05), largely because of high values in the stable asthmatic children who did not use anti-inflammatory treatment (0.8 micromol; p<0.01 compared to controls). We conclude that hydrogen peroxide is elevated in exhaled air condensate of children with stable asthma, and may reflect airway inflammation. 相似文献
153.
混酸PEG酯的合成及性能研究 总被引:3,自引:1,他引:2
用从大豆油脚水解制得的脂肪酸合成混酸聚乙二醇酯,通过测定酸值的方法确定了最佳反应时间为16h。与油酸聚乙二醇酯相比,混酸聚乙二醇酯具有更优良的乳化性、润湿性,可以替代油酸聚乙二醇酯用于纺织、农药和化妆品工业中。 相似文献
154.
155.
Modern digital communication systems rely heavily on baseband signal processing for in-phase and quadrature (I-Q) channels, and complex number processing in low-voltage CMOS has become a necessity for channel equalization, timing recovery, modulation, and demodulation. In this work, redundant binary (RB) arithmetic is applied to complex number multiplication for the first time so that an N-bit parallel complex number multiplier can be reduced to two RE multiplications (i.e., an addition of N RB partial products) corresponding to real and imaginary parts, respectively. This efficient RE encoding scheme proposed can generate RB partial products with no additional hardware and delay overheads. A prototype 8-bit complex number multiplier containing 11.5 K transistors is integrated on 1.05×1.33 mm2 using 0.8 μm CMOS. The chip consumes 90 mW with 2.5 V supply when clocked at 200 MHz 相似文献
156.
新型无灰分散剂的结构分析研究 总被引:3,自引:1,他引:2
通过薄膜渗析、红外光谱、凝胶渗透色谱、皂化反应等一系列手段建立了一套对无灰分散剂的系统分析方法,并利用该方法得到了一种新型无灰分散剂及其衍生物的平均结构、相对分子质量分布、分散度等重要结构信息,得出该新型无灰分散剂的结构与传统无灰分散剂的结构不同。 相似文献
157.
MH Collins R Yang Q Liu A Cnaan MD Pescovitz JL Grosfeld 《Canadian Metallurgical Quarterly》1997,32(4):555-559
The spectrum of photoaging in human skin shows a recognizable progression from subtle to profound, reflecting the various anatomic and structural changes that appear with aging skin. Qualification and quantification of these changes on both the macroscopic and microscopic levels enable the physician to select and evaluate appropriate therapeutic approaches to the problem of chronic solar damage. Comparative studies remain to be done that will further refine our abilities to match the right solutions to the recognized problems. 相似文献
158.
DJ Li K Ohsaki K Ii Q Ye Y Nobuto S Tenshin T Takano-Yamamoto 《Canadian Metallurgical Quarterly》1997,111(8):702-706
The present study evaluates histological characteristics of the soft tissue response to long-term implantation of Apaceram discs composed of dense hydroxyapatite in rats. Discs were implanted into the subcutaneous tissue of 76 rats for six to 20 months. Decalcified histological sections stained with haematoxylin and eosin (H & E) and Mallory's azan were examined. Different cell types surrounding implants were counted. The greatest proportion of macrophages was found at six months (13.5 per cent). This proportion gradually decreased to four per cent at 20 months. Small numbers of lymphocytes and foreign body giant cells were observed in every group, but neither neutrophils nor osteogenesis were observed in any specimens. Results of the present study and previous related studies indicate that despite reappearance of a small number of macrophages six months after implantation, Apaceram is useful for reconstructive surgery. 相似文献
159.
Chien S.Y.P. Xue L.Q. Palakal M. 《IEEE transactions on systems, man, and cybernetics. Part B, Cybernetics》1997,27(6):1007-1007
For a mobile robot to be practical, it needs to navigate in dynamically changing environments and manipulate objects in the environment with operating ease. The main challenges to satisfying these requirements in mobile robot research include the collection of robot environment information, storage and organization of this information, and fast task planning based on available information. Conventional approaches to these problems are far from satisfactory due to their requirement of high computation time. In this paper, we specifically address the problems of storage and organization of the environment information and fast task planning in the area of robotic research. We propose an special object-oriented data model (OODM) for information storage and management in order to solve the first problem. This model explicitly represents domain knowledge and abstracts a global perspective about the robot's dynamically changing environment. To solve the second problem, we introduce a fast task planning algorithm that fully uses domain knowledge related to robot applications and to the given environment. Our OODM based task planning method presents a general frame work and representation, into which domain specific information, domain decomposition methods and specific path planners can be tailored for different task planning problems. This method unifies and integrates the salient features from various areas such as database, artificial intelligence, and robot path planning, thus increasing the planning speed significantly 相似文献
160.
How senile plaques and neurofibrillary tangles are linked represents a major gap in our understanding of the pathophysiology of Alzheimer's disease. We characterized a hippocampal neuronal culture system in which tau undergoes maturation in vivo; rat neurons maintained in culture for more than 3 weeks replicated the splicing and phosphorylation changes that tau undergoes upon maturation in situ. Using this model system, we induced an Alzheimer-like neuritic dystrophy following the application of fibrillar beta-amyloid. The dystrophy consisted of focal distortions and swellings within the neurites and an altered phosphorylation of the adult tau isoforms. Fibrillar beta-amyloid induced the concomitant activation of MAP kinase and GSK3 beta. The aberrant activation of several signaling pathways may lead to the abnormal phosphorylation of tau and neuritic degeneration. 相似文献