Immunohistochemical and histochemical findings favoring the occurrence of autocrine/paracrine as well as nerve-related cholinergic effects in chronic painful patellar tendon tendinosis

The pathogenesis of the pain in patellar tendon tendinosis ("jumper's knee") is unclear. We have recently presented new information about the sensory nervous system in the human patellar tendon, but there is very little information regarding the possible occurrence of a cholinergic system in this tendon. In the present study, specimens of pain-free normal tendons and chronically painful tendinosis tendons were examined by different immunohistochemical and histochemical methods. Antibodies against the M(2) receptor, choline acetyltransferase (ChAT), and vesicular acetylcholine transporter (VAChT) were applied, and staining for demonstration of activity of acetylcholinesterase (AChE) was also utilized. It was found that immunoreactions for the M(2) receptor could be detected intracellularly in both blood vessel cells and tenocytes, especially in tendinosis specimens. Furthermore, in the tendinosis specimens, some tenocytes were seen to exhibit immunoreaction for ChAT and VAChT. AChE reactions were seen in fine nerve fibers associated with small blood vessels in both the normal control tendons and the tendinosis tendons. The observations suggest that there is both a nerve related and a local cholinergic system in the human patellar tendon. As ChAT and VAChT immunoreactions were detected in tenocytes of tendinosis tendons, these cells might be a source of local acetylcholine (Ach) production. As both tenocytes and blood vessel cells were found to exhibit immunoreactions for the M(2) receptor, it is likely that both of these tissue cells may be influenced by ACh. Thus, in conclusion, there appears to be an upregulation of the cholinergic system, and an occurrence of autocrine/paracrine effects in this system, in the tendinosis patellar tendon.     

Numerical modelling of plastic forming of aluminium single crystals

The current study focused on modelling of micro-deep drawing of aluminium single crystals using a physically-based crystal plasticity model. The approach consisted in modelling the gauge area located at the centre of the crystals with boundary conditions extracted from displacement fields measured experimentally by digital image correlation. Thanks to three different geometries of samples, three loading paths were considered: uniaxial tension, plane strain tension and equi-biaxial tension. Comparisons between simulations and experimental observations were performed in terms of displacement fields, strain fields and slip traces. Data obtained from the simulations also allowed analysis of the results in terms of crystal rotation, dislocation density evolution and accumulated slip. Reasonable agreement between experimental observations and simulations was generally obtained, except for the prediction of strain localisation which was only correct in the case of uniaxial and plane strain tension. Discrepancies and validity of the modelling approach are then discussed in terms of experimental inaccuracies and numerical approximations.     

Volumetric strain measurement of polymeric materials subjected to uniaxial tension

A novel method for measuring and calculating volumetric strain in circular cylindrical uniaxial tension samples made from polymeric materials is proposed. It is shown that special considerations must be taken when calculating volumetric strain when a sample is in a postnecking state. Solely based on surface data, the key feature of the proposed correction is that it allows for an inhomogeneous distribution of longitudinal strain through the diameter of the sample, where a more traditional approach would be to assume a homogeneous distribution. These two approaches are evaluated by applying them to data from a close‐to‐incompressible steel sample. Whereas the proposed method indicates only a small positive increase in volume, the assumption of a homogeneous distribution results in substantial negative volumetric strains. Applying the two methods to tension samples made from HDPE and PVC, where plastic dilatation is nonlinear, again shows an initial negative volumetric strain for HDPE with the assumption of a homogeneous longitudinal strain. The proposed method predicts close‐to‐zero early‐stage volumetric strain for the same test. The differences are more subtle for samples of PVC. Micrographs obtained with scanning electron microscope show that the dilatation of PVC is related to voiding of the material around filler particles, while the underlying mechanism for HDPE is less clear. The results indicate that earlier reports of negative volumetric strain in polymers subjected to uniaxial tension might be artefacts of the implicit assumption made when calculating the volumetric strain.     

Ventilation system design for a church pipe organ using numerical simulation and on-site measurement

Since old churches have had heating installed, more complaints have arisen of organs sounding out of tune. Sound frequency of organ pipes is dependent on air temperature. Old churches tend to have very large volumes, so are typically heated just before and during services in wintertime, in order to reduce energy usage. Warm air plumes rise at radiators and spread out into the room, finally reaching the cold organ casing where they cause a non-uniform temperature distribution within. If pipes standing in different temperature zones are played at the same time the organ sounds out of tune due to clearly audible beats (interference between two slightly different frequencies). The purpose of this study was to design a ventilation system inside the organ casing, able to create a uniform temperature distribution around the pipes. A computational fluid dynamic (CFD) model was developed for the St. Martin church in Oberesslingen, Germany. It was validated by on-site measurements that had been carried out in the organ casing of the church. Four organ divisions containing 300–500 pipes were represented by equivalent porous material zones. Their properties were determined using an auxiliary two-dimensional model of a staggered array consisting of 392 pipes. The effect of different ventilation system settings on the temperature field in the organ casing was examined. Best results were achieved by a system consisting of two ventilation lines together with a thin, synthetic net placed at the open interface between church and organ casing in order to reduce warm air inflow.     

On the description of ductile fracture in metals by the strain localization theory

Numerical simulations based on the bifurcation and imperfection versions of the strain localization theory are used in this paper to predict the failure loci of metals and applied to an advanced high strength steel subjected to proportional loading paths. The results are evaluated against the 3D unit cell analyses of Dunand and Mohr (J Mech Phys Solids 66(1):133–153, 2014. doi: 10.1016/j.jmps.2014.01.008) available in the literature. The Gurson porous plasticity model (Gurson in J Eng Mater Technol 99(1):2–15, 1977. doi: 10.1115/1.344340) is used to induce strain softening and drive the localization process. The effects of the void growth, void nucleation and void softening in shear are investigated over a large range of stress triaxialities and Lode parameters. A correlation between the imperfection and bifurcation results is established.     

A note on vowel centralization in stuttering and nonstuttering individuals

Inferences were made regarding vocal tract vowel space during fluently produced utterances through examination of the first two formant frequencies. Fifteen adult males served as subjects, representing separate groups of untreated and treated individuals who stutter and nonstuttering controls. The steady-state portion of formant one (F1) and formant two (F2) was examined in the production of various CVC tokens containing the vowels /i/, /u/, and /a/. Vocal tract vowel space was estimated three ways. The first analysis scheme involved measurement of formant frequency spacing. The second measure involved calculating the area of the vowel space triangle. The third measure was based on calculating the average Euclidean distance from each subject's midpoint "centroid" vocal tract position to the corresponding /i/, /u/, and /a/ points on the vowel triangle. The formant frequency spacing measures proved to be most revealing of group differences, with the untreated stutterers showing significantly greater vowel centralization than the treated group and control group. Discussion focuses on the vocal tract articulation characterizing fluent speech productions and possible treatment implications for persons who stutter.