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131.
David L. Felten Suzanne Y. Felten John R. Sladek Mary D. Notter Sonia L. Carlson Denise L. Bellinger Stanley J. Wiegand 《Journal of microscopy》1990,157(3):271-283
Formaldehyde-induced and glyoxylic-acid-induced fluorescence histochemistry permits the tissue localization of catecholamines in the central nervous system (CNS) and peripheral nervous system (PNS), and in culture. Counterstains such as ethidium bromide provide excellent background identification of specific innervated regions in both the CNS and the periphery. Use of fluorescence histochemistry with immunocytochemistry can elucidate catecholamine-peptide relationships. Gelatin-ink perfusion used with fluorescence histochemistry permits the investigation of neuro-vascular relationships and documentation of vascular and parenchymal compartmentation of innervation. Combined use of fluorescence histochemistry and retrograde tracing methods demonstrates the specific cellular sources of innervation of target regions. Micropunch neurochemical analysis provides quantitative data for correlation with fluorescence histochemistry within a target region of innervation, and micro-spectrofluorometric analysis provides a semi-quantitative evaluation of the amount of fluorophore within a target region or within specific subcellular compartments such as the cell body or terminals. 相似文献
132.
133.
The CLAS forward electromagnetic calorimeter 总被引:1,自引:0,他引:1
M. Amarian G. Asryan K. Beard W. Brooks V. Burkert T. Carstens A. Coleman R. Demirchyan Yu. Efremenko H. Egiyan K. Egiyan H. Funsten V. Gavrilov K. Giovanetti R. M. Marshall B. Mecking R. C. Minehart H. Mkrtchan M. Ohandjanyan Yu. Sharabian L. C. Smith S. Stepanyan W. A. Stephens T. Y. Tung C. Zorn 《Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment》2001,460(2-3):239-265
The CEBAF Large Acceptance Spectrometer (CLAS) at Jefferson Lab utilizes six iron-free superconducting coils to provide an approximately toroidal magnetic field. The six sectors are instrumented individually to form six independent spectrometers. The forward region (8°<θ<45°) of each sector is equipped with a lead–scintillator electromagnetic sampling calorimeter (EC), 16 radiation lengths thick, using a novel triangular geometry with stereo readout. With its good energy and position resolution, the EC is used to provide the primary electron trigger for CLAS. It is also used to reject pions, reconstruct π° and η decays and detect neutrons. This paper treats the design, construction and performance of the calorimeter. 相似文献
134.
To address the controversy of whether an intact procedural memory system alone can support the teaming of the recursive strategy for solving the Tower of Hanoi Puzzle, the authors tested 2 amnesic patients, H.M. and P.N. Contrary to the report of N. J. Cohen, H. Eichenbaum, B. S. Deacedo, and S. Corkin (1985), both patients failed to master the recursive strategy under the active-interaction condition. In contrast, normal control participants were able to master the strategy under identical testing conditions. The failure of H.M. and P.N. could not be attributed to the differences between the original and current testing conditions. In addition, neither patient showed frontal lobe dysfunction or impairment in procedural memory. Together with evidence provided by theoretical analyses of this puzzle as well as studies on normal participants, the authors conclude that declarative memory plays a vital role in the acquisition of the recursive strategy for solving the Tower of Hanoi Puzzle. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
135.
Bruce E. Maley Mary Gail Engle Suzanne Humphreys Debra A. Vascik Kimberly A. Howes Bruce W. Newton Robert P. Elde 《Microscopy research and technique》1990,15(1):20-33
The monoamines dopamine, noradrenaline, adrenaline, and serotonin as well as the diamine histamine have a widespread distribution in the central nervous system within synaptic terminals and nonsynaptic varicosities. In certain regions of the central nervous system the monoamines are contained in varicosities that have no synaptic specialization associated with them, suggesting a possible neuromodulatory role for some of the monoamines. The majority of monoamine labelled structures are synaptic terminals which are characterized by the presence of small, clear vesicles (40–60 nm) and large, granular vesicles (70–120 nm) within the terminal. A third population of vesicles—small, granular vesicles—which are visible only after histochemical staining, are probably the equivalent of the small, clear vesicles present after either autoradiographic or immunohistochemical labelling. Most monoamine containing terminals contact dendrites and dendritic spines and, less frequently, neuronal somata and other axons. Both asymmetrical and symmetrical membrane specializations are associated with monoaminergic terminals; however, asymmetrical contacts are the most frequent type found. These ultrastructural results indicate that monoamine containing terminals and varicosities in general share many common morphological features, but still have diverse functions. 相似文献
136.
Using a set of different search metrics and a set of model landscapes we theoretically and empirically study how ldquooptimalrdquo mutation rates for the simple genetic algorithm (SGA) depend not only on the fitness landscape, but also on population size and population state. We discuss the limitations of current mutation rate heuristics, showing that any fixed mutation rate can be expected to be suboptimal in terms of balancing exploration and exploitation. We then develop a mutation rate heuristic that offers a better balance by assigning different mutation rates to different subpopulations. When the mutation rate is assigned through a ranking of the population, according to fitness for example, we call the resulting algorithm a Rank GA. We show how this Rank GA overcomes the limitations of other heuristics on a set of model problems showing under what circumstances it might be expected to outperform a SGA with any choice of mutation rate. 相似文献
137.
Rice Robert W.; Phillips Suzanne M.; McFarlin Dean B. 《Canadian Metallurgical Quarterly》1990,75(4):386
In this study, a mail survey was used to measure pay satisfaction, current salary, 4 personal standards of comparison, and basic demographics for 169 mental health professionals. As predicted, pay satisfaction was determined by the simultaneous appraisal of current salary against several personal standards of comparison. Explained variance in pay satisfaction rose from 26.1% when only salary and demographics were used as predictors to 46.7% when discrepancy-related variables associated with 4 standards of comparison also were used. Furthermore, R–2 for the combined discrepancy-related variables associated with all 4 standards of comparison was significantly greater than R–2 for the discrepancy-related variables associated with any single standard. These discrepancy effects took both additive and nonadditive forms. Discrepancy effects were stronger when deserved salary or minimum salary was the standard of comparison than when other's salary or average salary was. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
138.
Pigeons were trained initially on a delayed matching task in which colors served as sample and comparison stimuli. During subsequent training, additional stimuli compounded with the sample signaled whether that trial involved a short (1 s) or a long (5 s) delay. In Experiment 1, miscuing reduced accuracy at the short delay markedly and tended to increase accuracy at the long delay slightly. Experiments 2 and 3 revealed a robust effect of cuing when the cues followed sample termination, thereby refuting the view that such cues evoke differential attention to the sample stimulus. Experiment 4 revealed that the cues did not influence rate of forgetting, and Experiment 5 revealed no effect of point of cue placement within the retention interval. It was concluded that cues correlated with retention interval length modulated matching accuracy independently of memory, perhaps by signaling different rates of reinforcement. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
139.
Manuela Tosin Dr. Lorena Betancor Dr. Elaine Stephens Dr. W. M. Ariel Li Peter F. Leadlay Prof. 《Chembiochem : a European journal of chemical biology》2010,11(4):539-546
Modular biocatalysis is responsible for the generation of countless bioactive products and its mining remains a major focus for drug discovery purposes. One of the enduring hurdles is the isolation of biosynthetic intermediates in a readily‐analysed form. We prepared a series of nonhydrolysable pantetheine and N‐acetyl cysteamine mimics of the natural (methyl)malonyl extender units recruited for polyketide formation. Using these analogues as competitive substrates, we were able to trap and off‐load diketide and triketide species directly from an in vitro reconstituted type I polyketide synthase, the 6‐deoxyerythronolide B synthase 3 (DEBS3). The putative intermediates, which were extracted in organic solvent and characterised by LC‐HR‐ESI‐MS, are the first of their kind and prove that small‐molecule chain terminators can be used as convenient probes of the biosynthetic process. 相似文献
140.
Flocculation,cell surface hydrophobicity and 3‐OH oxylipins in the SMA strain of Saccharomyces pastorianus 下载免费PDF全文
Three‐hydroxy‐oxylipins (3‐OH oxylipins) have been previously detected in brewing yeast production strains at flocculation onset. In this work, the SMA strain of Saccharomyces pastorianus was characterized during growth in a miniature fermentation assay by measuring flocculation and cell surface hydrophobicity (CSH). Proportions of 3‐OH oxylipin were also measured concurrently during growth in the miniature fermentation assay and a defined 3‐OH oxylipin extraction protocol using ethyl acetate is presented along with a novel derivatization and gas chromatography–mass spectrometry (GC‐MS) detection approach. When the SMA strain was grown in the assay, near maximal CSH and flocculation levels were achieved by a 36 h fermentation time. Under the same culture conditions, the oxylipin 3‐OH decanoic acid (3‐OH 10:0) was identified. This oxylipin could not be detected early in the fermentation, but elevated relative levels of 3‐OH 10:0 were reached by 36 h, coinciding with increased CSH levels. It was previously presumed that the formation of 3‐OH oxylipins at flocculation onset might increase the CSH. However, results from this study suggest that 3‐OH 10:0 may not contribute to cell wall hydrophobicity. The flocculation behaviour of the SMA strain was also monitored in the presence of 3‐OH 10:0, but exposure to this oxylipin did not impact the sedimentation of this yeast, suggesting that 3‐OH oxylipins may not act as mediators of quorum sensing in this strain. Copyright © 2015 The Institute of Brewing & Distilling 相似文献