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81.
Bühling S Wyrowski F 《Journal of the Optical Society of America. A, Optics, image science, and vision》2002,19(12):2414-2423
Diffractive beam-splitting elements are typically designed for replicating beams on positions belonging to an equidistant grid in the spatial spectrum. The parameter of the output grid follows directly from the period of the beam-splitter transmission through the grating equation. Our objective is to develop design strategies allowing a more accurate positioning of the replicated beams. Issues occurring when the output grid parameter is decreased below the output beam width are discussed and shown to be avoidable. Furthermore, a design algorithm is introduced, which permits an arbitrary positioning of the replicated beams. This algorithm is constructed for high computational efficiency by utilizing fast Fourier transform operations in the major part of its iterations. 相似文献
82.
Problem analysis is an important, but mainly neglected part of the problem‐solving process. The theory of inventive problem solving (TRIZ) offers an instrument called function analysis to conduct problem analysis, which is especially useful for exploring a problem. This process is based on an idea applied in different creativity techniques, saying that behind one problem statement many problem formulations may occur, which allows for evaluation and selection of those problem formulations before looking for problem solutions. The application of function analysis as a tool for teams is recommended for a number of reasons. In so doing so, two main questions arise: (i) Should function analysis be used with the help of an external moderator? and: (ii) Should it be applied with currently available special software? After introducing the basics of function analysis this article provides key findings deducted from an experiment addressing these questions. An important result is the remarkable learning effect, which occurs while using function analysis in teams. By applying function analysis in R&D, companies will be able to get deeper and team‐based problem explorations, which may lead to maturing problem solutions. 相似文献
83.
This study analysed how leadership and organizational support (LOS) influences creative knowledge environments for research
groups in biotechnology. A questionnaire distributed to 90 (97% responding) university and business company researchers resulted
in that leadership was rated higher than organizational support. First, leaders were more important to creativity than organizational
support. Secondly, LOS differed to a limited extent between members and leaders, universities and business companies and excellent
and less excellent groups. Thirdly, working freedom was rated higher in universities than in business companies. Fourthly,
group members perceived they were more encouraged to think freely in comparison to their group leaders. Finally, innovation
goals were more pronounced in excellent than less excellent groups. 相似文献
84.
The dynamic programming solution offered is of complexity O(n2) and is a significant improvement over the implicit enumeration procedure previously suggested by Arora and Rana in an article in the September 1980 issue of TRANSACTIONS. 相似文献
85.
Löfstedt C Zhu J Kozlov MV Buda V Jirle EV Hellqvist S Löfqvist J Plass E Franke S Francke W 《Journal of chemical ecology》2004,30(3):643-658
Under an artificial light:dark cycle, females of Lampronia capitella were observed calling, with extended terminal abdominal segments, during the first 2 hr of the photoperiod. Extracts of terminal abdominal segments from females elicited large electroantennographic responses from male antennae. Gas chromatography with electroantennographic detection revealed three active peaks. Based on comparison of retention times and mass spectra of synthetic standards, these compounds were identified as (Z,Z)-9,11-tetradecadienol and the corresponding acetate and aldehyde. The electroantennographic activity of the four geometric isomers of all three compounds was investigated, and the respective (Z,Z)-isomer was found to be the most active in all cases. Aldehydes generally elicited larger antennal responses than alcohols, whereas acetates were the least active compounds. A subtractive trapping assay in the field, based on a 13:26:100 micrograms mixture of (Z,Z)-9,11-tetradecadienal, (Z,Z)-9,11-tetradecadienyl acetate, and (Z,Z)-9,11-tetradecadienol confirmed that all three compounds are pheromone components. Subtraction of (Z,Z)-9,11-tetradecadienol from the blend completely eliminated its attractiveness, whereas the other two-component blends showed reduced activity. This is the first pheromone identification from the monotrysian superfamily Incurvarioidea, confirming that the common pheromones among ditrysian moths (long-chain fatty acid derivatives comprising alcohols, acetates, and aldehydes with one or more double bonds) is not an autapomorphy of Ditrysia, but a synapomorphy of the more advanced heteroneuran lineages. 相似文献
86.
87.
Ten Philosophical Problems in Belief Revision 总被引:4,自引:0,他引:4
88.
A novel liquid-liquid extraction (LLE) procedure was investigated for preparation of peptide and protein samples for matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). LLE using ethyl acetate as the water-immiscible organic solvent enabled segregation of hydrophobic and hydrophilic polypeptides in mixtures, thereby reducing the complexity of mass spectra obtained by MALDI MS. The LLE technique was optimized for rapid and sensitive in situ (on-target) sample preparation for MALDI MS analysis of proteins and peptides at low-picomole and subpicomole levels. Addition of MALDI matrix to the organic solvent enhanced the efficiency of the LLE-MALDI MS method for analysis of hydrophobic peptides and proteins. LLE-MALDI MS enabled the detection of the hydrophobic membrane protein bacteriorhodopsin as a component in a simple protein mixture. Peptide mixtures containing phosphorylated, glycosylated, or acylated peptides were successfully separated and analyzed by the in situ LLE-MALDI MS technique and demonstrate the potential of this method for enhanced separation and structural analysis of posttranslationally modified peptides in proteomics research. 相似文献
89.
Screening of biomarkers in rat urine using LC/electrospray ionization-MS and two-way data analysis 总被引:8,自引:0,他引:8
Idborg-Björkman H Edlund PO Kvalheim OM Schuppe-Koistinen I Jacobsson SP 《Analytical chemistry》2003,75(18):4784-4792
Biofluids, like urine, form very complex matrixes containing a large number of potential biomarkers, that is, changes of endogenous metabolites in response to xenobiotic exposure. This paper describes a fast and sensitive method of screening biomarkers in rat urine. Biomarkers for phospholipidosis, induced by an antidepressant drug, were studied. Urine samples from rats exposed to citalopram were analyzed using solid-phase extraction (SPE) and liquid chromatography mass spectrometry (LC/MS) analysis detecting negative ions. A fast iterative method, called Gentle, was used for the automatic curve resolution, and metabolic fingerprints were obtained. After peak alignment principal component analysis (PCA) was performed for pattern recognition, PCA loadings were studied as a means of discovering potential biomarkers. In this study a number of potential biomarkers of phospholipidosis in rats are discussed. They are reported by their retention time and base peak, as their identification is not within the scope of the study. In addition to the fact that it was possible to differentiate control samples from dosed samples, the data were very easy to interpret, and signals from xenobiotic-related substances were easily removed without affecting the endogenous compounds. The proposed method is a complement or an alternative to NMR for metabolomic applications. 相似文献
90.
Ke Feng Feng Yan Birgit W. Hütsch Sven Schubert 《Nutrient Cycling in Agroecosystems》2003,67(3):283-292
The effect of liming an acidic mineral soil (Dystric Nitosol from southern China), used for arable agriculture, on N2O emission was studied in an incubation experiment. After the soil pH had been raised from pH 4.4 to 5.2, 6.7 and 8.1, soil samples were either amended with NH4
+ and incubated aerobically, favoring nitrification or, after application of NO3
–, the incubation took place under anaerobic conditions, favoring denitrification. Gas sampling for N2O determination and soil analyses were performed at regular intervals up to 13 days. Under nitrification conditions only small N2O emission rates were observed (max. 6 g N kg–1 d–1) with significant differences between high and low pH values during the first 2 days of incubation. The nitrifying activity was low, even with high pH, and this, together with good aeration conditions, could partly explain the small N2O evolution. During denitrification, however, cumulative N2O emissions reached much higher values (1600 g N kg–1 in comparison to 40 g N kg–1 under nitrification conditions). N2O emission during denitrification was significantly enhanced by increasing soil pH. Under alkaline conditions (pH 8.1) a large nitrite accumulation occurred, which was in line with the highest nitrate reductase activity determined in this treatment. The limited availability of organic carbon is probably the main reason for the absence of further reduction of NO2
– to N2O or N2. At pH 6.7 the total N2O emission was slightly higher than at pH 8.1, although the start of pronounced emissions was retarded and only small amounts of NO2
– accumulated. Acid soil conditions caused either negligible (pH 4.4) or only small (pH 5.2) N2O emissions. It can be concluded that these kinds of soil, used alternatively for production of upland crops or paddy rice, are prone to high N2O emissions after flooding, particularly under neutral to alkaline conditions. In order to avoid major N2O evolution and accumulation of nitrite, which can be leached into groundwater, the pH should not be raised to values above 5.5–6. 相似文献