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排序方式: 共有471条查询结果,搜索用时 15 毫秒
51.
Phunsiri Suthiluk Sirinnapa Saranwong Sumio Kawano Sonthaya Numthuam & Takaaki Satake 《International Journal of Food Science & Technology》2008,43(1):160-165
The possibility of near infrared (NIR) spectroscopy to measure the amount of bacterial contamination in shredded cabbage was investigated. NIR measurements in the short wavelength region from 700 to 1100 nm were done using two types of saline solutions: one was used to stomach with the samples as the conventional method and the other was used to wash the outer surface of the sample to examine the possibility of a non‐destructive method. Partial least squares regression (PLS) was used to develop the equations for bacterial amount. Spectra from the stomacher solution and the washing solution produced similar results. Sufficiently accurate results could be obtained with the bias‐corrected standard error of prediction (SEPs) of 0.46 log CFU g?1 for the stomacher solution and 0.44 log CFU g?1 for the washing solution. NIR spectroscopy was clarified to be a rapid and non‐destructive method for prediction of bacterial contamination in shredded cabbage. 相似文献
52.
Yasutoshi Akiyama Yoshika Takenaka Tomoko Kasahara Takaaki Abe Yoshihisa Tomioka Pavel Ivanov 《International journal of molecular sciences》2022,23(21)
Under stress conditions, transfer RNAs (tRNAs) are cleaved by stress-responsive RNases such as angiogenin, generating tRNA-derived RNAs called tiRNAs. As tiRNAs contribute to cytoprotection through inhibition of translation and prevention of apoptosis, the regulation of tiRNA production is critical for cellular stress response. Here, we show that RTCB ligase complex (RTCB-LC), an RNA ligase complex involved in endoplasmic reticulum (ER) stress response and precursor tRNA splicing, negatively regulates stress-induced tiRNA production. Knockdown of RTCB significantly increased stress-induced tiRNA production, suggesting that RTCB-LC negatively regulates tiRNA production. Gel-purified tiRNAs were repaired to full-length tRNAs by RtcB in vitro, suggesting that RTCB-LC can generate full length tRNAs from tiRNAs. As RTCB-LC is inhibited under oxidative stress, we further investigated whether tiRNA production is promoted through the inhibition of RTCB-LC under oxidative stress. Although hydrogen peroxide (H2O2) itself did not induce tiRNA production, it rapidly boosted tiRNA production under the condition where stress-responsive RNases are activated. We propose a model of stress-induced tiRNA production consisting of two factors, a trigger and booster. This RTCB-LC-mediated boosting mechanism may contribute to the effective stress response in the cell. 相似文献
53.
Tatsunari Ohkubo Takaaki Shiina Kayoko Kawaguchi Daisuke Sasaki Rena Inamasu Yue Yang Zhuoqi Li Keizaburo Taninaka Masaki Sakaguchi Shoko Fujimura Hiroshi Sekiguchi Masahiro Kuramochi Tatsuya Arai Sakae Tsuda Yuji C. Sasaki Kazuhiro Mio 《International journal of molecular sciences》2022,23(23)
Membrane proteins play important roles in biological functions, with accompanying allosteric structure changes. Understanding intramolecular dynamics helps elucidate catalytic mechanisms and develop new drugs. In contrast to the various technologies for structural analysis, methods for analyzing intramolecular dynamics are limited. Single-molecule measurements using optical microscopy have been widely used for kinetic analysis. Recently, improvements in detectors and image analysis technology have made it possible to use single-molecule determination methods using X-rays and electron beams, such as diffracted X-ray tracking (DXT), X-ray free electron laser (XFEL) imaging, and cryo-electron microscopy (cryo-EM). High-speed atomic force microscopy (HS-AFM) is a scanning probe microscope that can capture the structural dynamics of biomolecules in real time at the single-molecule level. Time-resolved techniques also facilitate an understanding of real-time intramolecular processes during chemical reactions. In this review, recent advances in membrane protein dynamics visualization techniques were presented. 相似文献
54.
Xintong Wu Satoi Nagasawa Kasumi Muto Maiko Ueda Chitose Suzuki Takaaki Abe Atsushi Higashitani 《International journal of molecular sciences》2022,23(17)
Mitochonic Acid 5 (MA-5) enhances mitochondrial ATP production, restores fibroblasts from mitochondrial disease patients and extends the lifespan of the disease model “Mitomouse”. Additionally, MA-5 interacts with mitofilin and modulates the mitochondrial inner membrane organizing system (MINOS) in mammalian cultured cells. Here, we used the nematode Caenorhabditis elegans to investigate whether MA-5 improves the Duchenne muscular dystrophy (DMD) model. Firstly, we confirmed the efficient penetration of MA-5 in the mitochondria of C. elegans. MA-5 also alleviated symptoms such as movement decline, muscular tone, mitochondrial fragmentation and Ca2+ accumulation of the DMD model. To assess the effect of MA-5 on mitochondria perturbation, we employed a low concentration of rotenone with or without MA-5. MA-5 significantly suppressed rotenone-induced mitochondria reactive oxygen species (ROS) increase, mitochondrial network fragmentation and nuclear destruction in body wall muscles as well as endogenous ATP levels decline. In addition, MA-5 suppressed rotenone-induced degeneration of dopaminergic cephalic (CEP) neurons seen in the Parkinson’s disease (PD) model. Furthermore, the application of MA-5 reduced mitochondrial swelling due to the immt-1 null mutation. These results indicate that MA-5 has broad mitochondrial homing and MINOS stabilizing activity in metazoans and may be a therapeutic agent for these by ameliorating mitochondrial dysfunction in DMD and PD. 相似文献
55.
ON THE SPECIFIC RESISTANCE OF CAKES OF MICROORGANISMS 总被引:1,自引:0,他引:1
Kazuhiro Nakanishi Takaaki Tadokoro Ryuichi Matsuno 《Chemical Engineering Communications》1987,62(1):187-201
The mean specific resistance of the cakes of various microorganisms was evaluated by measurement of either a change in the amount of permeate with time or of steady-state flux under constant pressure. The mean specific resistance was different with different shapes and sizes of microorganisms. The large differences arose from different packing structures of the cake. The effect of a filter aid on the filtration rate and cake structure was studied experimentally and theoretically. The effects of a filter aid were best explained by a series model, in which a cake layer composed of microbial cells and a layer of randomly distributed microbial cells and filter aid are packed on the membrane surface in series with respect to the directions of permeation. 相似文献
56.
T. Shin-ike Takaaki Sakai Tetsuo Sakai G. Adachi J. Shiokawa 《Materials Research Bulletin》1978,13(11):1105-1110
Electrical and magnetic properties of the solid solutions LnV1?xMxO3 (Ln:La, Gd or Y, M:Cr or Fe) were studied in the temperature range 77–1000K. These solid solutions were all semiconductors. Their conductivity at room temperature decreased with Cr3+ or Fe3+ ion concentrations. The solid solutions LaV1?xMxO3 and YV1?xMxO3 (M:Cr or Fe) revealed an antiferromagnetism with a weak ferromagnetism and their ordering temperature increased with x. Most of the gadolinium-containing compounds were paramagnets in the measured temperature range. YV0.4Fe0.6O3 showed a thermal hysteresis at high temperatures. 相似文献
57.
Yamada Jun-Ichi Machida Susumu Mukai Takaaki Kano Hiroyuki Sugiyama Koichi 《Electronics letters》1979,15(19):596-597
Modulation experimenting on a buried-stripe (b.s.) InGaAsP laser emitting at 1.3 ?m was carried out up to 2 Gbit/s. The b.s. laser is free from relaxation oscillation, which degrades modulated optical pube waveforms, especially at high data rates. The repeater gain of 1.6 Gbit/s transmission systems is improved by 9 dB compared with previously reported experiments. Modulation characteristics up to 2 Gbit/s are reported. 相似文献
58.
59.
Yoshikawa J Amachi S Shinoyama H Fujii T 《Journal of Bioscience and Bioengineering》2007,103(5):491-493
beta-Fructofuranosidase I (FFase I) formed by Aureobasidium pullulans DSM 2404 was purified. The enzyme had a molecular weight of about 430 kDa, was not affected by various metal ions and showed high transfructosylating activity. The yield of fructooligosaccharides production using purified FFase I was 62%. 相似文献
60.
Kouya T Misawa K Horiuchi M Nakayama E Deguchi H Tanaka T Taniguchi M 《Journal of Bioscience and Bioengineering》2007,103(5):464-471
Production of a bifidogenic growth stimulator (BGS) by propionic acid bacteria was investigated under anaerobic and aerobic culture conditions. To measure the concentration of extracellular BGS produced by propionic acid bacteria, we evaluated the effects of bioassay conditions using Bifidobacterium longum as a test microorganism on the formation of a growth-stimulation zone. The diameter of the growth-stimulation zone was significantly affected by both the component concentrations and the pH of a bioassay medium. The optimum component concentrations and pH of a bioassay medium were one-half of the normal values and 8.5, respectively. Using the bioassay method, we can measure the concentration of BGS produced by propionic acid bacteria ranging in concentrations from 0.1 microg/l to 1 mg/l using 1,4-dihydroxy-2-naphthoic acid (DHNA) and 2-amino-3-carboxy-1,4-naphthoquinone (ACNQ) as standards. Of six dairy propionic acid bacterial strains tested, the four strains (Propionibacterium freudenreichii ET-3, P. shermanii PZ-3, P. acidipropionici JCM 6432, and P. jensenii JCM 6433) produced BGS at a concentration range of 4-23 mg/l under the anaerobic culture conditions. Analysis of high performance liquid chromatography (HPLC) showed that more than 70% of total BGS produced in supernatant samples was DHNA and no ACNQ was produced by the strains. The effect of oxygen supply on BGS production was investigated for the four BGS-producing strains. The aerobic conditions exerted in positive effects on BGS production by only P. acidipropionici JCM 6432. The concentration of BGS obtained in the aerobic cultivation of P. acidipropionici JCM 6432 was 1.3-fold than that in anaerobic cultivation. Different properties (BGS production as well as cell growth and glucose metabolism) occurring in response to the aerobic conditions were observed, depending on the propionic acid bacterial strain used. This paper is the first report on BGS production by propionibacterial strains except for P. freudenreichii. 相似文献