SHM1: A multicopy suppressor of a temperature-sensitive null mutation in the HMG1-like abf2 gene

HM, an HMG1-like mitochondrial DNA-binding protein, is required for maintenance of the yeast mitochondrial genome when cells are grown in glucose. To better understand the role of HM in mitochondria, we have isolated several multicopy suppressors of the temperature-sensitive defect associated with an abf2 null mutation (lacking HM protein). One of these suppressors, SHM1, has been characterized at the molecular level and is described herein. SHM1 encodes a protein (SHM1p) that shares sequence similarity to a family of mitochondrial carrier proteins. On glycerol medium, where mitochondrial function is required for growth, shm1 deletion mutants are able to grow, whereas shm1 abf2 double mutants are severely inhibited. These results suggest that SHM1p plays an accessory role to HM in the mitochondrion. The GenBank Accession Number for the SHM1 sequence is U08352.     

Evaporated Te on CdTe: A vacuum-compatible approach to making back contact to CdTe solar cell devices

A commonly used process for forming low-resistance contacts to thin-film p-type CdTe involves the formation of a Te layer by etching the CdTe film in a concentrated mixture of nitric and phosphoric acids. The authors compare evaporated Te back contacts with ‘control’ back contacts formed by the usual etching process, and demonstrate that evaporating Te onto a CdTe thin film is a viable process for forming a low-resistance contact. The best efficiency achieved for a CdTe solar cell made with an evaporated Te back contact is 12.1%, whereas the efficiency of the device made with the control back contact was 11.9%. The evaporation process offers numerous advantages over acid etching, most notably vacuum compatibility amenable to large-scale production of CdTe solar cell modules.     

Maternal Fructose Intake Causes Developmental Reprogramming of Hepatic Mitochondrial Catalytic Activity and Lipid Metabolism in Weanling and Young Adult Offspring

Excess dietary fructose is a major public health concern, yet little is known about its influence on offspring development and later-life disease when consumed in excess during pregnancy. To determine whether increased maternal fructose intake could have long-term consequences on offspring health, we investigated the effects of 10% w/v fructose water intake during preconception and pregnancy in guinea pigs. Female Dunkin Hartley guinea pigs were fed a control diet (CD) or fructose diet (FD; providing 16% of total daily caloric intake) ad libitum 60 days prior to mating and throughout gestation. Dietary interventions ceased at day of delivery. Offspring were culled at day 21 (D21) (weaning) and at 4 months (4 M) (young adult). Fetal exposure to excess maternal fructose intake significantly increased male and female triglycerides at D21 and 4 M and circulating palmitoleic acid and total omega-7 through day 0 (D0) to 4 M. Proteomic and functional analysis of significantly differentially expressed proteins revealed that FD offspring (D21 and 4 M) had significantly increased mitochondrial metabolic activities of β-oxidation, electron transport chain (ETC) and oxidative phosphorylation and reactive oxygen species production compared to the CD offspring. Western blotting analysis of both FD offspring validated the increased protein abundances of mitochondrial ETC complex II and IV, SREBP-1c and FAS, whereas VDAC1 expression was higher at D21 but lower at 4 M. We provide evidence demonstrating offspring programmed hepatic mitochondrial metabolism and de novo lipogenesis following excess maternal fructose exposure. These underlying asymptomatic programmed pathways may lead to a predisposition to metabolic dysfunction later in life.     

The utility of energy storage to improve the economics of wind–diesel power plants in Canada

Wind energy systems have been considered for Canada's remote communities in order to reduce their costs and dependence on diesel fuel to generate electricity. Given the high capital costs, low-penetration wind–diesel systems have been typically found not to be economic. High-penetration wind–diesel systems have the benefit of increased economies of scale, and displacing significant amounts of diesel fuel, but have the disadvantage of not being able to capture all of the electricity that is generated when the wind turbines operate at rated capacity.Two representative models of typical remote Canadian communities were created using HOMER, an NREL micro-power simulator to model how a generic energy storage system could help improve the economics of a high-penetration wind–diesel system. Key variables that affect the optimum system are average annual wind speed, cost of diesel fuel, installed cost of storage and a storage systems overall efficiency. At an avoided cost of diesel fuel of 0.30 $Cdn/kWh and current installed costs, wind generators are suitable in remote Canadian communities only when an average annual wind speed of at least 6.0 m/s is present. Wind energy storage systems become viable to consider when average annual wind speeds approach 7.0 m/s, if the installed cost of the storage system is less than 1000 $Cdn/kW and it is capable of achieving at least a 75% overall energy conversion efficiency. In such cases, energy storage system can enable an additional 50% of electricity from wind turbines to be delivered.     

Methods for determining operation life and reactivity depletion for space reactors with fast energy spectra

Space reactors with fast neutron energy spectrums are preferred for their compactness and high fission power density, but require a high fissile inventory. The operation life estimates of these reactors are important to mission planning. This paper examines a number of fuel depletion and neutronics code packages for determining the operation lives of two space reactors with hard fast neutron energy spectra. These are: the lithium-cooled, Sectored, Compact Reactor (SCoRe-S₁₁), and the submersion subcritical safe space (S^4) reactor, cooled with a He–Xe binary gas mixture (40 g/mol). This work investigated the code packages of Monteburns 2.0, MCNPX 2.6C and TRITON and validated their prediction with fuel depletion data for a PWR fuel bundle, with satisfactory results. The operation life predictions of the two space reactors using these code packages are compared with those calculated using a simplified method that couples MCNP5 to a burnup analysis model using the Simulink^® platform. This method considers only the 10 most probable low-Z and high-Z elements of the fission yield peaks plus ¹⁴⁹Sm, and neglects the depletion of fission products due to capture and radioactive decay. The simplified method requires significantly shorter running time and its predictions of the operation lives for the two space reactors are within 0.29–12.5% of those obtained using Monteburns 2.0 and MCNPX 2.6C code packages. This method, however, is not recommended for operation life predictions for space or commercial reactors with thermal neutron spectrums.     

Reactivity control options of space nuclear reactors

This paper compares two ex-core control options of the gas-cooled Submersion Subcritical Safe Space (S^4) reactor with a fast neutrons energy spectrum: (a) rotating BeO drums with 120° thin segments of enriched B₄C in the BeO radial reflector; and (b) sliding segments in the BeO radial reflector. Investigated are the effects on the beginning-of-life (BOL) excess reactivity, reactivity depletion rate and operation life, and the spatial neutron flux distributions and fission power profiles in the core. Also investigated is the effect of reducing the thickness of the enriched B₄C segments in the control drums on the BOL excess reactivity, when one or two of the 6 drums are stuck in the shutdown position. Reducing the thickness of the B₄C segments from 0.5 mm to 0.238 mm, with one drums stuck in the shutdown position, increases BOL cold and hot-clean excess reactivity from +$1.71 and +$0.47 to +$2.38 and +$0.89, respectively. These reactivity values are almost identical to those of the reactor with one of the six reflector segments stuck open in the shutdown position. Results also showed that the control options made little difference in the reactor performance. The power peaking in the reactor core with sliding reflector segments is slightly lower and the spatial power profiles are relatively flatter. The operation life of the reactor with a sliding reflector segments control, when operating at a nominal thermal power of 471 kW, is only 22 full power days longer than with rotating drums control.     

The Expression of Active CD11b Monocytes in Blood and Disease Progression in Amyotrophic Lateral Sclerosis

Monocytes expressing the inflammation suppressing active CD11b, a beta2 integrin, may regulate neuroinflammation and modify clinical outcomes in amyotrophic lateral sclerosis (ALS). In this single site, retrospective study, peripheral blood mononuclear cells from 38 individuals living with ALS and 20 non-neurological controls (NNC) were investigated using flow cytometry to study active CD11b integrin classical (CM), intermediate (IM) and non-classical (NCM) monocytes during ALS progression. Seventeen ALS participants were sampled at the baseline (V1) and at two additional time points (V2 and V3) for longitudinal analysis. Active CD11b+ CM frequencies increased steeply between the baseline and V3 (ANOVA repeated measurement, p < 0.001), and the V2/V1 ratio negatively correlated with the disease progression rate, similar to higher frequencies of active CD11b+ NCM at the baseline (R = −0.6567; p = 0.0031 and R = 0.3862; p = 0.0168, respectively). CD11b NCM, clinical covariates and neurofilament light-chain plasma concentration at the baseline predicted shorter survival in a multivariable and univariate analysis (CD11b NCM—HR: 1.05, CI: 1.01–1.11, p = 0.013. Log rank: above median: 43 months and below median: 21.22 months; p = 0.0022). Blood samples with the highest frequencies of active CD11b+ IM and NCM contained the lowest concentrations of soluble CD11b. Our preliminary data suggest that the levels of active CD11b+ monocytes and NCM in the blood predict different clinical outcomes in ALS.     

The chemical durability of glass and graphite–glass composite doped with cesium oxide

The role of temperature in determining the chemical stability of a waste form, as well as its leach rate, is very complex. This is because the dissolution kinetics is dependent both on temperature and possibility of different rate-controlling mechanisms that appear at different temperature regions. The chemical durability of Alumina-Borosilicate Glass (ABG) and Glass–Graphite Composite (GGC), bearing Tristructural Isotropic (TRISO) fuel particles impregnated with cesium oxide, were compared using a static leach test. The purpose of this study is to examine the chemical durability of glass–graphite composite to encapsulate coated fuel particles, and as a possible alternative for recycling of irradiated graphite. The test was based on the ASTM C1220-98 methodology, where the leaching condition was set at a temperature varying from 298 K to 363 K for 28 days. The release of cesium from ABG was in the permissible limit and followed the Arrhenius’s law of a surface controlled reaction; its activation energy (E_a) was 65.6 ± 0.5 kJ/mol. Similar values of Ea were obtained for Boron (64.3 ± 0.5) and Silicon (69.6 ± 0.5 kJ/mol) as the main glass network formers. In contrast, the dissolution mechanism of cesium from GGC was a rapid release, with increasing temperature, and the activation energy of Cs (91.0 ± 5 kJ/mol) did not follow any model related to carbon kinetic dissolution in water. Microstructure analysis confirmed the formation of Crystobalite SiO₂ as a gel layer and Cs⁺¹ valence state on the ABG surface.     

Environmental Control of Triplet Emission in Donor–Bridge–Acceptor Organometallics

Carbene‐metal‐amides (CMAs) are a promising family of donor–bridge–acceptor molecular charge‐transfer (CT) emitters for organic light‐emitting diodes. A universal approach is demonstrated to tune the energy of their CT emission. A blueshift of up to 210 meV is achievable in solid state via dilution in a polar host matrix. The origin of this shift has two components: constraint of thermally‐activated triplet diffusion, and electrostatic interactions between guest and polar host. This allows the emission of mid‐green CMA archetypes to be tuned to sky blue without chemical modifications. Monte‐Carlo simulations based on a Marcus‐type transfer integral successfully reproduce the concentration‐ and temperature‐dependent triplet diffusion process, revealing a substantial shift in the ensemble density of states in polar hosts. In gold‐bridged CMAs, this shift does not lead to a significant change in luminescence lifetime, thermal activation energy, reorganization energy, or intersystem crossing rate. These discoveries offer new insight into coupling between the singlet and triplet manifolds in CMA materials, revealing a dominant interaction between states of CT character. The same approach is employed using materials which have been chemically modified to alter the energy of their CT state directly, shifting the emission of sky‐blue chromophores into the practical blue range.