产品开发过程中的危机管理

产品开发过程的危机通常无法避免,而应对危机并非一件易事．欲使危机的负面影响降至最低,运用规范的方法应对危机尤显重要．针对目前产品开发的行为特征,提出产品开发过程中应对危机的行为模式．     

Modeling the soil nutrient balance of integrated agriculture-aquaculture systems in the Mekong Delta,Vietnam

This study quantifies soil nutrient balances of Integrated Agriculture-Aquaculture Systems in the Mekong Delta of Vietnam. Eleven farms were monitored to collect data on farm activities and nutrient inputs and outputs to compute these balances of the rice-based and high input fish system in O Mon district (R-HF); the rice-based and medium input fish system in Tam Binh district (R-MF); and the orchard-based and low input fish system in Cai Be district (O-LF). For the estimation, the Nutmon model has been adapted to the specific conditions in these integrated systems in Asia (Nutmon-Asia). New regression models of leaching and gaseous losses of nitrogen were applied to fields used for upland crops and paddy rice. Reference values were used for the assessment of nitrogen fixation in paddy soils, wet atmospheric deposition, and irrigation water. The results showed that farms in all three systems have nitrogen, phosphorus and potassium surpluses (84 kg N, 73 kg P, and 69 kg K ha⁻¹ year⁻¹). The O-LF system had the smallest nitrogen surplus while the smallest surplus of phosphorus and potassium was seen in the R-HF system. High surpluses of phosphorus and potassium were found in vegetable fields, whereas a negative potassium balance was found in the rice fields of all three systems. The positive farm nutrient balances indicate that it is likely that soil fertility will be maintained although there is a risk for environmental contamination.     

Input reconstruction for statistical‐based fault detection and isolation

In this work, an input reconstruction scheme for detecting and isolating sensor, actuator, and process faults is proposed. The scheme uses model‐based and statistical‐based FDI methods, which yields an improved analysis of abnormal operation conditions in chemical processes. The main advantage of the proposed approach over existing works lies in the reconstruction of system inputs and the subsequent estimation of fault signatures. This advantage is demonstrated through simulation examples and the analysis of recorded process data from a reactive batch distillation column. © 2011 American Institute of Chemical Engineers AIChE J, 2012     

On the use of peak-force tapping atomic force microscopy for quantification of the local elastic modulus in hardened cement paste

A surface of epoxy-impregnated hardened cement paste was investigated using a novel atomic force microscopy (AFM) imaging mode that allows for the quantitative mapping of the local elastic modulus. The analyzed surface was previously prepared using focussed ion beam milling. The same surface was also characterized by electron microscopy and energy-dispersive X-ray spectroscopy.We demonstrate the capability of this quantitative nanomechanical mapping to provide information on the local distribution of the elastic modulus (from about 1 to about 100 GPa) with a spatial resolution in the range of decananometers, that corresponds to that of low-keV back-scattered electron imaging. Despite some surface roughness which affects the measured nanomechanical properties it is shown that topography, adhesion and Young's modulus can be clearly distinguished.The quantitative mapping of the local elastic modulus is able to discriminate between phases in the cement paste microstructure that cannot be distinguished from the corresponding back-scattered electron images.     

Prototype and Chimera-Type Galectins in Placentas with Spontaneous and Recurrent Miscarriages

Galectins are galactose binding proteins and, in addition, factors for a wide range of pathologies in pregnancy. We have analyzed the expression of prototype (gal-1, -2, -7, -10) and chimera-type (gal-3) galectins in the placenta in cases of spontaneous abortions (SPA) and recurrent abortions (RA) in the first trimester. Fifteen placental samples from healthy pregnancies were used as a control group. Nine placentas were examined for spontaneous abortions, and 12 placentas for recurrent abortions. For differentiation and evaluation of different cell types of galectin-expression in the decidua, immunofluorescence was used. For all investigated prototype galectins (gal-1, -2, -7, -10) in SPA and RA placenta trophoblast cells the expression is significantly decreased. In the decidua/extravillous trophoblast only gal-2 expression was significantly lowered, which could be connected to its role in angiogenesis. In trophoblasts in first-trimester placentas and in cases of SPA and RA, prototype galectins are altered in the same way. We suspect prototype galectins have a similar function in placental tissue because of their common biochemical structure. Expression of galectin 3 as a chimera type galectin was not found to be significantly altered in abortive placentas.     

The Significance of Epithelial-to-Mesenchymal Transition for Circulating Tumor Cells

Epithelial to mesenchymal transition (EMT) is a process involved in embryonic development, but it also plays a role in remote metastasis formation in tumor diseases. During this process cells lose their epithelial features and adopt characteristics of mesenchymal cells. Thereby single tumor cells, which dissolve from the primary tumor, are enabled to invade the blood vessels and travel throughout the body as so called “circulating tumor cells” (CTCs). After leaving the blood stream the reverse process of EMT, the mesenchymal to epithelial transition (MET) helps the cells to seed in different tissues, thereby generating the bud of metastasis formation. As metastasis is the main reason for tumor-associated death, CTCs and the EMT process are in the focus of research in recent years. This review summarizes what was already found out about the molecular mechanisms driving EMT, the consequences of EMT for tumor cell detection, and suitable markers for the detection of CTCs which underwent EMT. The research work done in this field could open new roads towards combating cancer.