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101.
Haverinen-Shaughnessy U Hyvärinen A Putus T Nevalainen A 《The Science of the total environment》2008,399(1-3):19-27
Based on seven case studies of buildings that underwent different degrees of moisture and mold damage remediation, we aimed to develop methodology for assessment of the success of the remediation process. Methods used in gauging the success included technical monitoring of performance of building structures and heating, ventilation and air conditioning (HVAC) systems, microbial monitoring of indoor air quality (IAQ), and health effects studies of building occupants. The assessment was based on measurable change in the situations before and after remediation. Based on technical monitoring, remediation was successful in three cases, with partial improvement noted in three cases, whereas no remediation was conducted in one case. Based on microbial monitoring, improvement was detected in one, partial improvement in two and no improvement in two cases, whereas no follow-up was conducted in two cases. Health effect studies (mainly self-reported health status) showed improvement in one case, partial improvement in two cases, and no improvement in two cases, whereas no follow-up was conducted in one case, and in one case, follow-up failed due to low response rate. The results illustrate that it is possible to monitor the effects of remediation using various metrics. However, in some cases, no improvement could be observed in IAQ or occupant health, even if the remediation was considered technically successful, i.e. the remediation was fully completed as recommended. This could be due to many reasons, including: 1) all damage may not have been addressed adequately; 2) IAQ or health may not have been perceived improved regardless of remediation; and/or 3) the methods used may not have been sensitive/specific enough to detect such improvement within the 6-12 months follow-up periods after completion of the remediation. There is a need to further develop tools for monitoring and assessment of the success of moisture damage remediation in buildings. 相似文献
102.
“To Catch or Not to Catch”: Microcapsule‐Based Sandwich Assay for Detection of Proteins and Nucleic Acids 下载免费PDF全文
Sujit K. Verma Amanda Amoah Ulla Schellhaas Mathias Winterhalter Sebastian Springer Tatiana A. Kolesnikova 《Advanced functional materials》2016,26(33):6015-6024
The development of new assays that specifically detect intermediate or final products of biotechnological processes or multiple analytes in biomedical research is important for diagnostics and biotechnology. This study presents a microcapsule‐based sandwich assay for detection of proteins and nucleic acids using flow cytometry as an optical readout. The main component of the assay are robust chemically cross‐linked microcapsules that are coated with adaptor proteins such as protein A or streptavidin. In the first approach, the ability of detecting the blood cancer biomarker beta‐2 microglobulin in the fM to pM concentration range with the help of protein A‐coated capsules is demonstrated. In the second approach, streptavidin‐coated capsules are used for detection of nucleic acids in the nM concentration range. The developed assay allows rapid quantitative analyte measurement, while providing high sensitivity and selectivity at very small sample quantities. In the future, protein A‐ and streptavidin‐coated microcapsules can be used as universal tools for detection of a broad range of analytes. 相似文献
103.
Anne Steen-Hansen Ragni Fjellgaard Mikalsen Ulla Eidissen Jensen 《Fire Technology》2018,54(6):1585-1608
A bench-scale experimental setup has been used to study the conditions necessary for smouldering ignition in four types of loose-fill wood fibre thermal insulation, and to study the development of the smouldering process. The products varied with regard to wood species, grain size and fire retardant chemical additives. The test material was placed in an insulated open top container and heated from below. Temperatures within the sample and mass loss were measured during the tests. Both the fibre size and the level of added fire retardant seem to influence the smouldering ignition. Two different types of smouldering were identified in this study. Materials undergoing smouldering Type 1 obtained maximum temperatures in the range 380°C to 440°C and a total mass loss of 40 wt% to 50 wt%. Materials undergoing smouldering Type 2 obtained maximum temperatures in the range 660°C to 700°C and a total mass loss of 80 wt% to 90 wt%. This implies that Type 2 smouldering involves secondary char oxidation, which represents a risk for transition to flaming combustion and thereby a considerable fire hazard. This has been an exploratory project and the results must therefore be considered as indicative. The findings may, however, have implications for fire safety in the practical use of loose-fill wood fibre insulation in buildings, and further experimental studies should be performed with this in mind to obtain more knowledge about the topic. 相似文献
104.
Anne T Saber Nicklas R Jacobsen Jette Bornholdt Sanna L Kjær Marianne Dybdahl Lotte Risom Steffen Loft Ulla Vogel Håkan Wallin 《Particle and fibre toxicology》2006,3(1):4-8
Background
Particulate air pollution has been associated with lung and cardiovascular disease, for which lung inflammation may be a driving mechanism. The pro-inflammatory cytokine, tumor necrosis factor (TNF) has been suggested to have a key-role in particle-induced inflammation. 相似文献105.
Abstract. A hidden Markov regime is a Markov process that governs the time or space dependent distributions of an observed stochastic process. We propose a recursive algorithm for parameter estimation in a switching autoregressive process governed by a hidden Markov chain. A common approach to the recursive estimation problem is to base the estimation on suboptimal modifications of Kalman filtering techniques. The main idea in this paper is to use the maximum likelihood method and from this develop a recursive EM algorithm. 相似文献
106.
Rachel I. Adams Hanna Leppänen Anne M. Karvonen José Jacobs Alicia Borràs-Santos Maria Valkonen Esmeralda Krop Ulla Haverinen-Shaughnessy Kati Huttunen Jan-Paul Zock Anne Hyvärinen Dick Heederik Juha Pekkanen Martin Täubel 《Indoor air》2021,31(6):1952-1966
Moisture-damaged buildings are associated with respiratory symptoms and underlying diseases among building occupants, but the causative agent(s) remain a mystery. We first identified specific fungal and bacterial taxa in classrooms with moisture damage in Finnish and Dutch primary schools. We then investigated associations of the identified moisture damage indicators with respiratory symptoms in more than 2700 students. Finally, we explored whether exposure to specific taxa within the indoor microbiota may explain the association between moisture damage and respiratory health. Schools were assessed for moisture damage through detailed inspections, and the microbial composition of settled dust in electrostatic dustfall collectors was determined using marker-gene analysis. In Finland, there were several positive associations between particular microbial indicators (diversity, richness, individual taxa) and a respiratory symptom score, while in the Netherlands, the associations tended to be mostly inverse and statistically non-significant. In Finland, abundance of the Sphingomonas bacterial genus and endotoxin levels partially explained the associations between moisture damage and symptom score. A few microbial taxa explained part of the associations with health, but overall, the observed associations between damage-associated individual taxa and respiratory health were limited. 相似文献
107.
Kröger V. Hietikko M. Angove D. French D. Lassi U. Suopanki A. Laitinen R. Keiski R. L. 《Topics in Catalysis》2007,42(1-4):409-413
Effects of phosphorus poisoning on the activity of powdered exhaust gas catalyst components containing different oxides and
platinum were studied. The decrease in the catalytic activity was associated with phosphorus compounds formed with the catalyst
components. The poisoned samples were studied using XRD and FTIR-ATR techniques. The same compounds, phosphates of cerium,
zirconium, aluminium and titanium, have also been detected in vehicle aged diesel catalyst converters. 相似文献
108.
Liuliu Du Virpi Leivo Tadas Prasauskas Martin Tubel Dainius Martuzevicius Ulla Haverinen‐Shaughnessy 《Indoor air》2019,29(4):686-697
We assessed 45 multifamily buildings (240 apartments) from Finland and 20 (96 apartments) from Lithuania, out of which 37 buildings in Finland and 15 buildings in Lithuania underwent energy retrofits. Building characteristics, retrofit activities, and energy consumption data were collected, and Indoor Air Quality (IAQ) parameters, including carbon monoxide (CO), nitrogen dioxide (NO2), formaldehyde (CH2O), selected volatile organic compounds (benzene, toluene, ethylbenzene, and xylenes (BTEX), radon, and microbial content in settled dust were measured before and after the retrofits. After the retrofits, heating energy consumption decreased by an average of 24% and 49% in Finnish and Lithuanian buildings, respectively. After the retrofits of Finnish buildings, there was a significant increase in BTEX concentrations (estimated mean increase of 2.5 µg/m3), whereas significant reductions were seen in fungal (0.6‐log reduction in cells/m2/d) and bacterial (0.6‐log reduction in gram‐positive and 0.9‐log reduction in gram‐negative bacterial cells/m2/d) concentrations. In Lithuanian buildings, radon concentrations were significantly increased (estimated mean increase of 13.8 Bq/m3) after the retrofits. Mechanical ventilation was associated with significantly lower CH2O concentrations in Finnish buildings. The results and recommendations presented in this paper can inform building retrofit studies and other programs and policies aimed to improve indoor environment and health. 相似文献
109.
Julie A Bourdon Anne T Saber Nicklas R Jacobsen Keld A Jensen Anne M Madsen Jacob S Lamson Håkan Wallin Peter Møller Steffen Loft Carole L Yauk Ulla B Vogel 《Particle and fibre toxicology》2012,9(1):1-14
Background
Widespread occupational exposure to carbon black nanoparticles (CBNPs) raises concerns over their safety. CBNPs are genotoxic in vitro but less is known about their genotoxicity in various organs in vivo.Methods
We investigated inflammatory and acute phase responses, DNA strand breaks (SB) and oxidatively damaged DNA in C57BL/6 mice 1, 3 and 28 days after a single instillation of 0.018, 0.054 or 0.162 mg Printex 90 CBNPs, alongside sham controls. Bronchoalveolar lavage (BAL) fluid was analyzed for cellular composition. SB in BAL cells, whole lung and liver were assessed using the alkaline comet assay. Formamidopyrimidine DNA glycosylase (FPG) sensitive sites were assessed as an indicator of oxidatively damaged DNA. Pulmonary and hepatic acute phase response was evaluated by Saa3 mRNA real-time quantitative PCR.Results
Inflammation was strongest 1 and 3 days post-exposure, and remained elevated for the two highest doses (i.e., 0.054 and 0.162 mg) 28 days post-exposure (P < 0.001). SB were detected in lung at all doses on post-exposure day 1 (P < 0.001) and remained elevated at the two highest doses until day 28 (P < 0.05). BAL cell DNA SB were elevated relative to controls at least at the highest dose on all post-exposure days (P < 0.05). The level of FPG sensitive sites in lung was increased throughout with significant increases occurring on post-exposure days 1 and 3, in comparison to controls (P < 0.001-0.05). SB in liver were detected on post-exposure days 1 (P < 0.001) and 28 (P < 0.001). Polymorphonuclear (PMN) cell counts in BAL correlated strongly with FPG sensitive sites in lung (r = 0.88, P < 0.001), whereas no such correlation was observed with SB (r = 0.52, P = 0.08). CBNP increased the expression of Saa3 mRNA in lung tissue on day 1 (all doses), 3 (all doses) and 28 (0.054 and 0.162 mg), but not in liver.Conclusions
Deposition of CBNPs in lung induces inflammatory and genotoxic effects in mouse lung that persist considerably after the initial exposure. Our results demonstrate that CBNPs may cause genotoxicity both in the primary exposed tissue, lung and BAL cells, and in a secondary tissue, the liver. 相似文献110.
Margarete Schlosser Sabrina Fröls Ulla Hauf Ingo Sethmann Stefanie Schultheiss Felicitas Pfeifer Hans‐Joachim Kleebe 《Journal of the American Ceramic Society》2013,96(2):412-419
Two processing methods were successfully combined to obtain Ag‐modified calcium phosphate scaffolds with antibacterial properties: (i) hydrothermal conversion of macroporous biogenic carbonates and (ii) vapor transport sintering. Hydrothermal conversion of two precursor materials, i.e., coral skeletons and sea urchin spines, resulted in the pseudomorphic replacement of highly porous calcium carbonates by calcium phosphate scaffolds. Vapor transport sintering of these scaffolds within a reactive AgCl atmosphere facilitated near net‐shape processing accompanied by the condensation of finely dispersed Ag‐bearing particles over the scaffold's surface. Chemical and phase compositions were analyzed using WDXRF, XRD, and DRIFTS (FTIR), and the microstructure development was characterized by SEM and TEM imaging. The dissolution kinetics of Ag+ ions in aqueous solution was determined and growth inhibition experiments with Gram‐positive and Gram‐negative bacteria were performed to assess the antibacterial properties of Ag‐modified ceramics. 相似文献