Melatonin and Myo-Inositol: Supporting Reproduction from the Oocyte to Birth

Human pregnancy is a sequence of events finely tuned by several molecular interactions that come with a new birth. The precise interlocking of these events affecting the reproductive system guarantees safe embryo formation and fetal development. In this scenario, melatonin and myo-inositol seem to be pivotal not only in the physiology of the reproduction process, but also in the promotion of positive gestational outcomes. Evidence demonstrates that melatonin, beyond the role of circadian rhythm management, is a key controller of human reproductive functions. Similarly, as the most representative member of the inositol’s family, myo-inositol is essential in ensuring correct advancing of reproductive cellular events. The molecular crosstalk mediated by these two species is directly regulated by their availability in the human body. To date, biological implications of unbalanced amounts of melatonin and myo-inositol in each pregnancy step are growing the idea that these molecules actively contribute to reduce negative outcomes and improve the fertilization rate. Clinical data suggest that melatonin and myo-inositol may constitute an optimal dietary supplementation to sustain safe human gestation and a new potential way to prevent pregnancy-associated pathologies.     

City as Political Form: Four Archetypes of Urban Transformation

Pier Vittorio Aureli focuses on the category of archetype as an alternative to the idea of type. Four examples – the axial streets of Renaissance Rome, the 17th-century Parisian place, the 19th-century independent block in Berlin and the 20th-century Viennese superblock – are explored here to describe the emergence of modern urban forms that explicitly embody power relations.     

Beta-glucans improve growth, viability and colonization of probiotic microorganisms

Probiotics, prebiotics and synbiotics are frequently-used components for the elaboration of functional food. Currently, most of the commercialized probiotics are limited to a few strains of the genera Bifidobacteria, Lactobacillus and Streptococcus, most of which produce exopolysaccharides (EPS). This suggests that the beneficial properties of these microorganisms may be related to the biological activities of these biopolymers. In this work we report that a 2-substituted-(1,3)-β-d-glucan of non-dairy bacterial origin has a prebiotic effect on three probiotic strains. Moreover, the presence of this β-d-glucan potentiates in vitro adhesion of the probiotic Lactobacillus plantarum WCFS1 to human intestinal epithelial cells.     

Secretome Survey of Human Plexiform Neurofibroma Derived Schwann Cells Reveals a Secreted form of the RARRES1 Protein

To bring insights into neurofibroma biochemistry, a comprehensive secretome analysis was performed on cultured human primary Schwann cells isolated from surgically resected plexiform neurofibroma and from normal nerve tissue. Using a combination of SDS-PAGE and high precision LC-MS/MS, 907 proteins were confidently identified in the conditioned media of Schwann cell cultures combined. Label free proteome profiling revealed consistent release of high levels of 22 proteins by the four biological replicates of NF1 Schwann cell cultures relative to the two normal Schwann cell cultures. Inversely, 9 proteins displayed decreased levels in the conditioned media of NF1 relative to normal Schwann cells. The proteins with increased levels included proteins involved in cell growth, angiogenesis and complement pathway while proteins with decreased levels included those involved in cell adhesion, plasminogen pathway and extracellular matrix remodeling. Retinoic acid receptor responder protein-1 (RARRES1), previously described as an integral membrane tumor suppressor, was found exclusively secreted by NF1 Schwann cells but not by normal Schwann cells. All-trans retinoic acid modulated secretion of RARRES1 in a dose dependent manner. This study shows altered secretion of key proteins in NF1 derived Schwann cells. The potential implication of these proteins in neurofibroma biology is discussed.     

Questioning the couch: Historical and clinical perspectives.

Since the beginning of the last century, when Freud introduced the couch in the psychoanalytic room, a number of “objects” have been removed or renovated, yet the couch has almost always remained in place, at least officially. This article has two distinct aims. The first is to offer a reconstruction of the psychoanalytic history of this element of the setting. The second is to rethink the couch, saving it from being reduced to a mere icon. Recent contributions coming from affective neurosciences, infant research, and psychotherapy research seem to question the utility of this element and the nature of the very mechanisms of its therapeutic action. The authors wonder whether the use of the couch is primarily protective for the therapist rather than necessarily helpful for all patients. Taking into account these observations implies bringing the couch out from a silent dimension and reflecting on its role in the conception of therapeutic action and mental functioning. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

On road experimental tests of hydrogen/natural gas blends on transit buses

The promise of reducing harmful and CO₂ emissions by focusing on hydrogen-methane blends (HCNG) have recently attracted the interest of vehicle manufacturers and transport operators. Several experiments have been conducted in laboratory facilities to assess the potential of HCNG blends in order to decrease the exhaust emissions. This paper reports the results of experimental tests performed at the ENEA Casaccia Research Center aiming to evaluate the energy and environmental performances of a CNG vehicle when fuelled with a hydrogen-methane blend. Two buses for urban transit service were fuelled with HCNG blends with different percentage of hydrogen (5%, 10%, 15%, 20% and 25% of hydrogen by volume). A 100% methane gas was used as reference to compare the advantages and disadvantages that can be derived from the use of HCNG blends. Road tests have been carried out by running fixed tracks, which are representative of urban and suburban driving cycles. Vehicles were powered with a lean burn engine whose setup - based on ignition advance angle, has been tuned for controlling the NO_x emissions. CO₂ emissions have been investigated to evaluate the leverage effect based on an increased CO₂ reduction resulting from an increased engine efficiency.     

Core-annular flow in horizontal and slightly inclined pipes: Existence, pressure drops, and hold-up

An analysis of high viscosity oil/water flow in horizontal and slightly inclined pipe is presented. We propose experimental flow maps – in particular we focus on the core-annular flow pattern boundary – pressure drops, and oil hold-up measurements. Experimental data are used to validate some models present in literature. Since hold-up measurement in liquid–liquid systems is particularly challenging and, therefore, only few data are available in literature, they are analyzed and commented in detail. The slip ratio computed by hold-up data is also provided and analyzed.     

The Organization of the Quorum Sensing luxI/R Family Genes in Burkholderia

Members of the Burkholderia genus of Proteobacteria are capable of living freely in the environment and can also colonize human, animal and plant hosts. Certain members are considered to be clinically important from both medical and veterinary perspectives and furthermore may be important modulators of the rhizosphere. Quorum sensing via N-acyl homoserine lactone signals (AHL QS) is present in almost all Burkholderia species and is thought to play important roles in lifestyle changes such as colonization and niche invasion. Here we present a census of AHL QS genes retrieved from public databases and indicate that the local arrangement (topology) of QS genes, their location within chromosomes and their gene neighborhoods show characteristic patterns that differ between the known Burkholderia clades. In sequence phylogenies, AHL QS genes seem to cluster according to the local gene topology rather than according to the species, which suggests that the basic topology types were present prior to the appearance of current Burkholderia species. The data are available at http://net.icgeb.org/burkholderia/.     

Rheology of a wet, fragmenting granular flow and the riddle of the anomalous friction of large rock avalanches

The effective friction coefficient of rock avalanches diminishes gradually as a function of the avalanche volume. Large rock avalanches can reach run-out distances as long as ten times the fall height, despite the fact that the physics of friction would indicate a run-out only a little greater than the fall height. Numerous suggestions have been put forward to explain this remarkable departure from the predictions of both small-scale experiments and basic theory. It is shown here that accounting for rock fragmentation within the avalanche in combination with the presence of water, leads to results in line with the data.