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W. Wagner F. Gruttmann W. Sprenger 《International journal for numerical methods in engineering》2001,51(11):1337-1359
This paper presents a finite element method to simulate growing delaminations in composite structures. The delamination process, using an inelastic material law with softening, takes place within an interface layer having a small, but non‐vanishing thickness. A stress criterion is used to detect the critical points. To prevent mesh‐dependent solutions a regularization technique is applied. The artificial viscosity leads to corresponding stiffness matrices which guarantee stable equilibrium iterations. The essential material parameter which describes the delamination process is the critical energy release rate. The finite element calculations document the robustness and effectivity of the developed model. Extensive parameter studies are performed to show the influence of the introduced geometrical and material quantities. Copyright © 2001 John Wiley & Sons, Ltd. 相似文献
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Sarah Schneider Mariana Gutiérrez Tatyana Sandalova Dr. Gunter Schneider Prof. Dr. Pere Clapés Dr. Georg A. Sprenger Prof. Dr. Anne K. Samland Dr. 《Chembiochem : a European journal of chemical biology》2010,11(5):681-690
Recently, we reported on a transaldolase B variant (TalB F178Y) that is able to use dihydroxyacetone (DHA) as donor in aldol reactions. In a second round of protein engineering, we aimed at improving the affinity of this variant towards nonphosphorylated acceptor aldehydes, that is, glyceraldehyde (GA). The anion binding site was identified in the X‐ray structure of TalB F178Y where a sulfate ion from the buffer was bound in the active site. Therefore, we performed site‐directed saturation mutagenesis at three residues forming the putative phosphate binding site, Arg181, Ser226 and Arg228. The focused libraries were screened for the formation of D ‐fructose from DHA and d,l ‐GA by using an adjusted colour assay. The best results with respect to the synthesis of D ‐fructose were achieved with the TalB F178Y/R181E variant, which exhibited an at least fivefold increase in affinity towards d,l ‐GA (KM=24 mM ). We demonstrated that this double mutant can use D ‐GA, glycolaldehyde and the L ‐isomer, L ‐GA, as acceptor substrates. This resulted in preparative synthesis of D ‐fructose, D ‐xylulose and L ‐sorbose when DHA was used as donor. Hence, we engineered a DHA‐dependent aldolase that can synthesise the formation of polyhydroxylated compounds from simple and cheap substrates at preparative scale. 相似文献
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The research field of structural health monitoring (SHM) in the realm of civil engineering has emerged rapidly. SHM concepts are based on integrated sensors and actuators to evaluate the structural state. Beside common structural response methods and other nondestructive testing techniques, wave-based ultrasonic techniques are widely used especially because of their flexibility. Monitoring cable structures such as overhead transmission lines or stay cables in suspension bridges is one objective of those wave-based methods. These structures are subject to aging, corrosion and other static and dynamic loads (e.g., wind, temperature). The cylindrical structures act as waveguides whereby monitoring of large distances with a single ultrasonic transducer is possible. However, the wave propagation is multimodal and dispersive, which complicates analysis of the wave motion and development of monitoring applications. This work addresses several aspects of the propagation of guided waves in cylinders, especially the analysis of reflection and transmission at discontinuities using finite element and boundary element methods. 相似文献
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The Dutch Association for Paediatric Medicine has formulated guidelines regarding influenza vaccination of children with pulmonary disease. Influenza virus is the most frequent cause of airway infections in humans over two years of age. It may lead to serious morbidity in children with pulmonary disease: exacerbations, (transient) disturbances in pulmonary function, and symptoms lasting weeks, but mortality is probably very low. The effects of influenza vaccination of children with pulmonary disease are similar to those in normal healthy children. A positive long-term effect on the asthma has never been demonstrated. It is advised that children with moderate to severe asthma who require treatment to be vaccinated against influenza every year. If the first vaccination ever occurs before the age of six years, it should be followed by a booster vaccination after four weeks. In both instances, a full vaccination dose should be administered. 相似文献
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Contrast Agent Incorporation into Silicone Enables Real‐Time Flow‐Structure Analysis of Mammalian Vein‐Inspired Soft Pumps
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Michael Loepfe Christoph M. Schumacher Cornelia H. Burri Wendelin J. Stark 《Advanced functional materials》2015,25(14):2129-2137
The construction of machines consisting essentially of soft parts is a nascent and multidisciplinary research field between material science, machine engineering, and robotics. Soft silicones represent a promising class of materials for the creation of a vast multitude of biologically inspired entities. In the present work, a new type of mammalian vein‐inspired soft silicone pump is introduced and characterized, which is fabricated by virtual lost‐wax casting of 3D‐printed injection molds. These pumps can be actuated pneumatically or by internal gas combustion and preserve their functionality even after a freezing/unfreezing cycle. The possibility of using medical examination methods such as ultrasonic imaging to directly access flow information inside soft pumps is shown. Based on soda lime glass microspheres, a method is demonstrated to enhance contrast properties during such color online Doppler imaging for a detailed understanding of the inner fluid‐structure interactions. 相似文献
79.
Wendelin J. Wright M.W. Samale T.C. Hufnagel M.M. LeBlanc J.N. Florando 《Acta Materialia》2009,57(16):4639-4648
We have made measurements of the temporal and spatial features of the evolution of strain during the serrated flow of Pd40Ni40P20 bulk metallic glass tested under quasistatic, room temperature, uniaxial compression. Strain and load data were acquired at rates of up to 400 kHz using strain gages affixed to all four sides of the specimen and a piezoelectric load cell located near the specimen. Calculation of the displacement rate requires an assumption about the nature of the shear displacement. If one assumes that the entire shear plane displaces simultaneously, the displacement rate is approximately 0.002 m s–1. If instead one assumes that the displacement occurs as a localized propagating front, the velocity of the front is approximately 2.8 m s?1. In either case, the velocity is orders of magnitude less than the shear wave speed (~2000 m s?1). The significance of these measurements for estimates of heating in shear bands is discussed. 相似文献
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