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71.
Floorbeams of orthotropic plated bridge decks are generally elements with a low slenderness, especially in the case of railway bridges. When loaded, such elements generate not only flexural deformations but shear deformations as well. Depending on the configuration, these shear deformations can be considerably large and should certainly not be neglected. In a design according to the Pelikan-Esslinger method, this deformation is taken into account in the second stage of the calculation of the orthotropic deck. At this stage, the additional bending moments, shear forces and floorbeam reactions due to the floorbeam flexibility are evaluated. The deflection of a directly loaded floorbeam creates a distribution of the load to adjacent non-loaded floorbeams. In addition, the deflection will affect the longitudinal ribs, increasing the sagging moments at midspan and decreasing the bending moments at the supports of the ribs provided by the floorbeams. In this paper a method is developed to take the shear deformation in the floorbeam into account, thus correcting the Pelikan-Esslinger method. The correction is especially important since the floorbeam web is frequently further weakened by cutouts for the longitudinal ribs and in some cases additional cope holes. The floorbeam web resisting the shear deformation is further reduced by these openings, increasing the importance of shear deformations even for more slender floorbeams. The validity of the proposed method is checked by full finite element calculation using shell elements which inherently comprise shear deformation. The effect of the correction on the calculations in the second stage of the calculation are relatively small for the floorbeam reactions; on the contrary, it can be quite large for the additional bending moments in the longitudinal ribs. In that case, the effect is worth considering.  相似文献   
72.
ABSTRACT

Given that online platforms disrupt established industries and challenge existing institutions, they can only be successful if their innovation becomes both legal and legitimate. This requires ‘institutional work’ that changes perceptions and regulations within society. Rather than only focussing on the online platform as the sole agent engaging in institutional work, our study analyses institutional work as a collective process. We investigate the case of home-sharing platform Airbnb and the process of institutional change its introduction prompted regarding short-term rental in Amsterdam, London and New York. We find, contrary to the popular view of online platforms as disruptive entrepreneurs, that the platform mainly focusses on creating new institutions rather than disrupting existing ones, and that users and non-users undertake most of the institutional work activities. We also show that different types of actors carry out different types of institutional work suggesting that the process of institutional work is highly distributed.  相似文献   
73.
We have performed numerical calculations of the noise in voltage-biased superconducting transition edge-based X-ray microcalorimeters, using a finite-element model. Details of the model are discussed, as well as results for different absorber geometries. The results are in agreement with an analytical model and show that the amount of internal thermal fluctuation noise can be reduced by using a segmented absorber. The simulation also agrees well with experimental data, which, for our detectors, contain no major unidentified noise sources. Furthermore, the discrepancy between the (small-signal) theoretical and the measured energy resolutions for 5.9 keV X-rays, for our sensors typically a factor of 2, is explained by a more accurate modelling of the sensor responsivity, taking into account the large signal behaviour.  相似文献   
74.
Motivated by several observations of the degree of linear polarization of skylight in the oxygen A (O(2)A) band that do not yet have a quantitative explanation, we analyze the influence of aerosol altitude, microphysics, and optical thickness on the degree of linear polarization of the zenith skylight in the spectral region of the O(2)A band, between 755 to 775 nm. It is shown that the degree of linear polarization inside the O(2)A band is particularly sensitive to aerosol altitude. The sensitivity is strongest for aerosols within the troposphere and depends also on their microphysical properties and optical thickness. The polarization of the O(2)A band can be larger than the polarization of the continuum, which typically occurs for strongly polarizing aerosols in an elevated layer, or smaller, which typically occurs for depolarizing aerosols or cirrus clouds in an elevated layer. We show that in the case of a single aerosol layer in the atmosphere a determination of the aerosol layer altitude may be obtained. Furthermore, we show limitations of the aerosol layer altitude determination in case of multiple aerosol layers. To perform these simulations we developed a fast method for multiple scattering radiative transfer calculations in gaseous absorption bands including polarization. The method is a combination of doubling-adding and k-binning methods. We present an error estimation of this method by comparing with accurate line-by-line radiative transfer simulations. For the Motivated by several observations of the degree of linear polarization of skylight in the oxygen A (O(2)A) band that do not yet have a quantitative explanation, we analyze the influence of aerosol altitude, microphysics, and optical thickness on the degree of linear polarization of the zenith skylight in the spectral region of the O(2)A band, between 755 to 775 nm. It is shown that the degree of linear polarization inside the O(2)A band is particularly sensitive to aerosol altitude. The sensitivity is strongest for aerosols within the troposphere and depends also on their microphysical properties and optical thickness. The polarization of the O(2)A band can be larger than the polarization of the continuum, which typically occurs for strongly polarizing aerosols in an elevated layer, or smaller, which typically occurs for depolarizing aerosols or cirrus clouds in an elevated layer. We show that in the case of a single aerosol layer in the atmosphere a determination of the aerosol layer altitude may be obtained. Furthermore, we show limitations of the aerosol layer altitude determination in case of multiple aerosol layers. To perform these simulations we developed a fast method for multiple scattering radiative transfer calculations in gaseous absorption bands including polarization. The method is a combination of doubling-adding and k-binning methods. We present an error estimation of this method by comparing with accurate line-by-line radiative transfer simulations. For the O(2)A band, the errors in the degree of linear polarization are less than 0.11% for transmitted light, and less than 0.31% for reflected light. band, the errors in the degree of linear polarization are less than 0.11% for transmitted light, and less than 0.31% for reflected light.  相似文献   
75.
Urinary modified nucleosides have a potential role as cancer biomarkers, and most of the methods used in their study have utilized low-pressure phenylboronate affinity chromatography materials for the purification of the cis-diol-containing nucleosides. In this study, a boronate HPLC column was surprisingly shown not to trap the nucleosides as would be expected from experience with the classic Affigel 601 resin but showed only partial selectivity toward cis-diol groups while other groups exhibited better retention. In aprotic conditions, trapping of nucleosides was possible; however, the selectivity toward cis-diol-containing compounds was lost with the Lewis basicity of available nitrogens being the main determinant of retention. The experimental findings are compared to and confirmed by DFT calculations.  相似文献   
76.
77.
Roos W  Ulmer J  Gräter S  Surrey T  Spatz JP 《Nano letters》2005,5(12):2630-2634
We combined biochemical and topographical patterning to achieve motor-driven microtubule gliding on top of microfabricated pillar arrays with limited and controllable surface interactions of gliding microtubules. Kinesins immobilized on pillar heads pushed microtubules from the top of one micropillar to the next bridging up to 20 mum deep gaps filled with buffer solution. Distances of more than 10 mum were by-passed, and microtubule buckling was occasionally observed. The velocity distributions of microtubules gliding on poly(dimethylsiloxane) (PDMS) pillars, on flat PDMS, and on glass were found to be different, most likely due to topological and/or chemical differences between the substrates. We also used pillar arrays to suspend cross-linked microtubule networks, whose structural characteristics were governed by the topographical characteristics of the pillar pattern. These experiments open new possibilities to study the dynamics and the self-organization of motor/microtubule networks in defined topologically structured environments.  相似文献   
78.
In current cognitive psychology, naming latencies are commonly measured by electronic voice keys that detect when sound exceeds a certain amplitude threshold. However, recent research (e.g., K. Rastle & M. H. Davis, 2002) has shown that these devices are particularly inaccurate in precisely detecting acoustic onsets. In this article, the authors discuss the various problems and solutions that have been put forward with respect to this issue and show that classical voice keys may trigger several tens of milliseconds later than acoustic onset. The authors argue that a solution to this problem may come from voice keys that use a combination of analogue and digital noise (nonspeech sound) detection. It is shown that the acoustic onsets detected by such a device are only a few milliseconds delayed and correlate highly (up to .99) with reaction time values obtained by visual waveform inspection. (PsycINFO Database Record (c) 2010 APA, all rights reserved)  相似文献   
79.
A new method is presented that enables sensitive measurement of free concentrations of radiolabeled ligands. Additionally, protein binding of radiochemicals in complex matrixes can be determined with this new technique that combines negligible depletion solid-phase microextraction (nd-SPME) with liquid scintillation counting (LSC) as detection. [3H]Estradiol was taken as an example compound. Possible matrix effects of protein on fiber uptake kinetics were studied. No matrix effect was found, either by fouling of the fiber, or by changed uptake kinetics. The validity of the method was shown in the determination of the affinity constant (Ka) of estradiol for human serum albumin (HSA). The Ka was estimated at 8.9 x 10(4) M(-1), which corresponds well with literature values. This study shows that nd-SPME is suitable to study the free concentration and protein binding of [3H]estradiol. The method described in this paper combines the advantages of nd-SPME with the advantages of radiolabeled analytes, creating a timesaving, simple, and sensitive analytical tool that will be particularly useful in complex matrixes containing many potential interferences for chromatographic methods.  相似文献   
80.
Biogrouting is a biological ground improvement method, in which microorganisms are used to induce carbonate precipitation in the subsurface in order to increase the strength and stiffness of granular soils. In this paper the results of a large-scale experiment (100?m3) are presented, in which the feasibility of biogrouting as a ground improvement method is investigated using techniques and equipment similar to those used in potential applications. In situ geophysical measurements were used to monitor the biogrouting process during treatment and indicated that the stiffness had increased significantly after one day of treatment. The results of unconfined compressive strength tests on samples which were excavated after treatment were used to assess the distribution of mechanical properties throughout the cemented sand body, which correlated quite well with the results of the in situ geophysical measurements. The stiffness increase could be quantified as a function of the injected volume of grouting agents and the distance from the injection points. These results will serve as an important benchmark for future applications of biomediated ground improvement.  相似文献   
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