Expression of recombinant antigens in Escherichia coli: application on immunochemical studies of Schistosoma mansoni tegumental antigens

Sm15 and Sm13 are recognized by antibodies from mice protectively vaccinated with tegumental membranes, suggesting a potential role in protective immunity. In order to raise antibodies for immunochemical investigations, the genes for these antigens were expressed in pGEX and pMal vectors so that comparisons could be made among different expression systems and different genes. The fusion proteins corresponding to several parts of the gene for the precursor of Sm15 failed in producing antibodies recognizing the parasite counterpart. On the other hand, antibodies raised against Sm13 MBP-fusion proteins recognized the 13 kDa tegumental protein. Thus the peculiarities of the gene of interest are important and the choice of the expression system must sometimes be decided on an empirical basis.     

A Survey of Inverse Surface Design From Light Transport Behavior Specification

Inverse surface design problems from light transport behavior specification usually represent extremely complex and costly processes, but their importance is well known. In particular, they are very interesting for lighting and luminaire design, in which it is usually difficult to test design decisions on a physical model in order to avoid costly mistakes. In this survey, we present the main ideas behind these kinds of problems, characterize them, and summarize existing work in the area, revealing problems that remain open and possible areas of further research.     

Self-Organized Monitoring in Ad-Hoc Networks

We propose in this paper a self-organized monitoring architecture for mobile ad-hoc networks based on a selective scheme where subsets of nodes are managed. These nodes are determined based on their network behavior in order to favor subsets of well-connected nodes. The key idea is to relax the requirements of the management plane, and to use these manageable subsets to monitor the performance of the overall network. We therefore propose a new performance metric to be monitored, in order to estimate the capability of ad-hoc nodes to communicate end-to-end in the network. Extensive simulations show how different parameters affect this metric.     

Molecular simulation of temperature-programmed desorption

We use accelerated molecular dynamics to probe the thermal desorption of n-alkanes from the Au(1 1 1) and C(0 0 0 1) surfaces. Studying an alkane series ranging from CH₄ to C₁₆H₃₄, we find that the desorption prefactor increases with increasing chain length for the short chains until a certain chain length is reached when it becomes essentially constant and independent of chain length. We can understand the dependence of the preexponential factor on alkane chain length in terms of conformational changes within the alkane molecules. For the shorter molecules, the desorption temperatures probed in experimental temperature-programmed desorption studies lie below those for which torsional motion is activated. Thus, the short alkanes can be treated as rigid rods, and the loss in translational and rotational entropy upon adsorption leads to a preexponential factor that increases with increasing chain length. As the alkane chain length increases, the binding energy and the experimental desorption temperatures also increase. Thus, torsional motion is activated to an extent that increases with increasing chain length. This torsional activation increases the entropy of adsorption and counteracts the entropy decrease due to a loss of translation and rotation. This leads to a virtually constant prefactor for sufficiently long chains. Our findings are consistent with experimental data for the thermal desorption of alkanes from the Au(1 1 1), Pt(1 1 1), MgO(1 0 0) and C(0 0 0 1) surfaces.     

Multifractal analysis of non-conformal repellers: a model case

For a model class of repellers of non-conformal expanding maps, we obtain sharp lower bounds for the dimension spectra of the Birkhoff averages of a Hölder continuous function. As in the well-studied case of conformal dynamics, the bounds are also expressed here in terms of the thermodynamic formalism.     

A simplified approach for sheet forming processes design

A new approach is developed to improve sheet forming parts and sheet forming processes design. In order to apply classical optimization methods, an energetic characterization of the global transformation of an elasto-plastic structure is proposed. After a brief review of thermostatics in elastoplastic laws, an energetic criterion is defined. With a geometrical definition of sheet forming processes, it is shown how to use this criterion to find the optimal displacement path between a flat blank and the part shape. Then, the classical assumptions of rigid-plastic behaviour and of thin shell geometry are introduced. Eventually, the criterion is used to define a formability criterion, for use at the preliminary design stage of parts. This paper concludes with numerical applications of the approach and analysis of formability criterion applications.     

ATM switching and CPE adaptation in the North Carolina InformationHighway

This article describes the approach which has been used to implement a sector of the information highway throughout the state of North Carolina, with specific attention focused on the functionality of the equipment that comprises the North Carolina Information Highway (NCIH). This implementation of this information highway is based on ATM technology, used in both the switching systems of the network and the customer premises equipment (CPE). The large ATM switching systems provide the extensive number of connections that are predicted for the information highway. Multiple services can also be supported on the ATM switches. The information streams for these different services can all be converted into ATM format using the appropriate ATM adaptation layer for transport through the ATM network. By being able to support multiple services, and transport them across common ATM facilities, the ATM switches offer the NCIH great flexibility in the applications that can be provided to the customers. Use of the ATM technology allows the NCIH to provide the backbone for various services. To provide the distance learning service application and other applications on the NCIH, ATM capabilities are also being provided in CPE on a service multiplexer