Molecular Identification of the G-Protein Genes and Their Expression Profiles in Response to Nitrogen Deprivation in Brassica napus

Heterotrimeric guanine nucleotide binding protein (G-protein) consisting of Gα, Gβ, and Gγ subunits is one of the key signal transducers in plants. Recent studies indicated that G-protein has been proposed as an important mediator of nitrogen responses in rice, wheat, and Arabidopsis. However, little is known about these G-proteins in Brassica napus (B. napus), except for three identified G-proteins, BnGA1, BnGB1, and BnGG2. Therefore, the aim of the present study is to characterize the members of the G-protein gene family in allotetraploid B. napus and to analyze their expression profiles in response to nitrogen deprivation. In total, 21 G-protein family members were identified in B. napus, encoding two Gα, six Gβ, and 13 Gγ. Sequence and phylogenetic analyses showed that although genome-wide triploid events increased the number of genes encoding Gα, Gβ, and Gγ subunits, the gene structure and protein properties of the genes encoding each G-protein subunit were extremely conserved. Collinearity analysis showed that most G-protein genes in B. napus had syntenic relationships with G-protein members of Arabidopsis, Brassica rape (B. rapa), and Brassica oleracea (B. oleracea). Expression profile analysis indicated that Gα and C-type Gγ genes (except BnGG10 and BnGG12 were highly expressed in flower and ovule) were barely expressed in most organs, whereas most Gβ and A-type Gγ genes tended to be highly expressed in most organs. G-protein genes also showed various expression patterns in response to nitrogen-deficient conditions. Under nitrogen deficiency, Gα and five C-type Gγ genes were upregulated initially in roots, while in leaves, Gα was downregulated initially and five C-type Gγ genes were highly expressed in different times. These results provide a complex genetic dissection of G-protein genes in B. napus, and insight into the biological functions of G-protein genes in response to nitrogen deficiency.     

异构无线网络的认证算法

本文针对建立在对等方式下的融合网络提出了一种新的认证和密钥更新算法.认证包括三个方面:非漫游状态下的完整认证、漫游状态下的完整认证以及切换状态下的快速认证.密钥更新包括两个方面:非漫游状态下的密钥更新和漫游状态下的密钥更新.在算法中,通过引入认证中心,不仅实现了网络对用户身份的认证,而且实现了节点对网络身份的认证,由此保证了双方身份的合法性;同时为了实现切换的无缝性,采用节点对认证数据包进行中继转发的方式,减少了切换时的认证时间.性能分析表明,该算法能够有效地抵御常见攻击.     

机床进给系统爬行问题的研究

在高精密机床研制中,进给系统的精度对机床的加工精度影响很大,本文对机床爬行问题进行了分析和研究,对实际应用有较好的参考和指导作用.     

Ba2Zn2Fe12O22铁氧体和弛豫铁电体

采用陶瓷制备工艺成功制备了Ba2Zn2Fe12O22铁氧体和铁电性PMZNT的复合材料,并借助于TMA,XRD,SEM和VSM技术,对所制备复合材料的共烧特性、相组成、显微结构和磁性能进行了研究.结果表明,Ba2Zn2Fe12O22铁氧体和PMZNT的烧成收缩和烧成收缩率存在明显的差异复合材料由Ba2Zn2Fe12O22和PMZNT两相组成;复合材料具有良好的显微结构.复合材料呈现软磁铁氧体典型的磁滞回线.     

Experimental modal analysis on damage of skeleton in brake of airplane

１　ＩＮＴＲＯＤＵＣＴＩＯＮＴｈｅｓｋｅｌｅｔｏｎｉｓａｒｉｎｇｐｌａｔｅｗｉｔｈｍａｎｙｈｏｌｅｓ．Ｔｈｅｃｏｎｖｅｎｔｉｏｎａｌｑｕａｌｉｔｙｃｏｎｔｒｏｌｃｈｅｃｋｏｆｓｋｅｌｅｔｏｎｉｓｍｅｃｈａｎｉｃａｌｓｔｒｅｎｇｔｈａｎｄｈａｒｄｎｅｓｓｔｅｓｔｉｎｇ,ｉｔｉｓａｄｅｓｔｒｕｃｔｉｖｅｏｎｅａｎｄｓａｍｐｌｅｃｈｅｃｋｃａｎｏｎｌｙｂｅｕｔｉｌｉｚｅｄ,ｔｈｕｓ,ｉｔｓｆａｉｌｕｒｅｉｓｕｎａｖｏｉｄａｂｌｅ．Ｕｌｔｒａｓｏｎｉｃｔｅｓｔｉｎｇｃａｎｎｏｔｂｅｐｅｒｆｏｒｍｅｄｂ…     

电加热器预热在水轮机转轮焊接中的应用

介绍了450t大型转轮焊接预热技术的改进措施,在保证转轮整体加热的基础上取得了良好的效果,节约了大量的材料及能源费用。     

伺服系统中USB人机接口的设计与实现

针对传统伺服系统串口通信速率慢、可扩展性差等不足,设计了应用于伺服系统中的USB人机接口.采用32位浮点DSP芯片TMS320F28335为控制核心,PDIUSBD 12为USB器件,并基于实时操作系统DSP/BIOS实现了USB驱动程序以及伺服管理软件,通过与PC端管理软件协同工作,完成伺服系统与PC主机的交互.经验证USB接口数据传输的正确性和可靠性能满足伺服系统中数据实时显示以及系统调试的要求,为伺服中数据采集系统以及高级调试工具的开发奠定基础.     

Numerical analysis of the molten pool vibration mode for arc-ultrasonic keyhole plasma arc welding

With considering the characteristics of keyhole plasma arc welding (K-PAW ),the resonant frequency between arc and molten pool was solved in the frequency range 15000 Hz to 72000 Hz by finite element m...     

A Multifunctional and Fast-Response Lysosome-Targetable Fluorescent Probe for Monitoring pH and Isoxaflutole

A new chemosensor, namely N-(2-morpholinoethyl)acetamide-4-morpholine-1,8-naphthimide (MMN), was designed and synthesized through an amidation reaction. MMN was fabricated as a multifunctional fluorescent probe for monitoring pH and isoxaflutole. MMN exhibited excellent stability in MeCN/H₂O (v/v, 9/1), with an obvious “off–on” fluorescence response toward pH changes due to intramolecular charge transfer (ICT), where the linear response ranges of MMN in the weakly acidic system were from 4.2 to 5.0 and from 5.0 to 6.0 with apparent pK_a = 4.62 ± 0.02 and 5.43 ± 0.02. Based on morpholine as the lysosome targetable unit, MMN could selectively locate lysosomes in live cells. MMN also successfully detected the presence of H⁺ in test papers. Finally, MMN could specifically recognize isoxaflutole at a detection limit of 0.88 μM. A possible sensing mechanism was identified based on density function theory calculations. These results indicate that MMN could be a superior potential chemosensor for detecting pH and isoxaflutole selectively and sensitively and could be used in real sample detection.