Preferential exclusion of sucrose from recombinant interleukin-1 receptor antagonist: role in restricted conformational mobility and compaction of native state

Understanding the mechanism for sucrose-induced protein stabilization is important in many diverse fields, ranging from biochemistry and environmental physiology to pharmaceutical science. Timasheff and Lee [Lee, J. C. & Timasheff, S. N. (1981) J. Biol. Chem. 256, 7193-7201] have established that thermodynamic stabilization of proteins by sucrose is due to preferential exclusion of the sugar from the protein's surface, which increases protein chemical potential. The current study measures the preferential exclusion of 1 M sucrose from a protein drug, recombinant interleukin 1 receptor antagonist (rhIL-1ra). It is proposed that the degree of preferential exclusion and increase in chemical potential are directly proportional to the protein surface area and that, hence, the system will favor the protein state with the smallest surface area. This mechanism explains the observed sucrose-induced restriction of rhIL-1ra conformational fluctuations, which were studied by hydrogen-deuterium exchange and cysteine reactivity measurements. Furthermore, infrared spectroscopy of rhlL-1ra suggested that a more ordered native conformation is induced by sucrose. Electron paramagnetic resonance spectroscopy demonstrated that in the presence of sucrose, spin-labeled cysteine 116 becomes more buried in the protein's interior and that the hydrodynamic diameter of the protein is reduced. The preferential exclusion of sucrose from the protein and the resulting shift in the equilibrium between protein states toward the most compact conformation account for sucrose-induced effects on rhIL-1ra.     

Airway stents. Present and future

Tracheobronchial stent insertion is a relatively new technique used to palliate or cure central airways obstruction. When performed by experienced thoracic endoscopists, this procedure is both safe and effective, even if complications of indwelling stents may require repeat endoscopic intervention. Although proven clinically beneficial to many patients, airway prostheses have not yet been the subject of large comparative case studies or randomized controlled investigations. Reports are often experiential and anecdotal. Further research is needed to determine the effects of stenting on survival in patients with malignant airway disease and its degree of success in patients with benign airway strictures. Only then will airway stents truly deserve their place alongside other therapies for tracheobronchial obstruction.     

预处理工艺对硬质合金与金刚石膜间粘结力的影响

在两种经不同预处理的硬质合金YG8基底上，采用微波等离子体化学气相沉积法，在微波功率2kW，压强4.0kPa和6.5kPa，CH4和H2流量分别为1.6cm/s和100.0cm/s的条件下生长金刚石薄膜。利用X射线衍射检测了金刚石薄膜是否存在，用拉曼光谱分析了薄膜的质量，用金相显微镜观察了薄膜的洛氏硬度压痕，标定并比较了不同预处理工艺膜与基底的结合力。实验结果表明，不同的预处理方法对于粘结力的影响不大，最主要的因素是钴含量的多少。     

试论会计电算化系统的内部控制

阐述了会计电算化系统产生和发展对内产控制的影响，以及内部控制的实施方法。     

中国石油未来油气勘探重点领域分析

根据近几年来的研究认识与勘探成果分析,未来几年中国石油油气勘探主要集中于如下五大重点领域:大面积低渗透岩性油气藏、成熟区复杂隐蔽油气藏、前陆盆地复杂构造油气藏、海相碳酸盐岩油气藏和渤海湾滩海第三系油气藏。     

太阳热水器:解决我国百姓生活热水的唯一有效途径

提起小康水平，人们往往想到人均收入、人均住房面积、人均受教育程度等，往往忽视了更能体现人们生活品质的另一项重要指标：人均生活热水消耗量。目前，发达国家家庭热水能源消耗占总能耗的比例为２５％～３０％。我国是一个能耗大国，人均常规能源占有量极低，２１世纪面临着经济的可持续发展与节能环保的双重课题。用可再生清洁能源解决全部或部分家用热水，节能环保意义重大，推广普及太阳热水器决不是无关紧要的，而是唯一有效途径。就现状而言，解决普通百姓生活热水的途径主要有使用电热水器、燃气热水器和太阳热水器三种。我们从能…     

ADS7822及其在CO气体浓度监测仪中的应用

在介绍TI公司的12位串行、高速、微功耗A/D转换器ADS7822工作原理的基础上,以ADS7822在CO气体浓度监测仪的前向通道设计中的应用为例,讨论了它与AT2051单片机的硬件接口问题,给出了利用ADS7822进行A/D转换的51汇编语言程序,并做了详细注释.串行、微功耗及小型封装使ADS7822非常适合电池供电的智能便携式仪器采用.     

尿素解吸废液及甲醇残液联合处理工艺的应用

安阳化学工业集团公司(以下简称安化集团)现有2套200 kt/a尿素装置及1套60 kt/a甲醇装置.尿素及甲醇装置在生产过程中会产生大量的尿素解吸废液和甲醇残液,废液中的氨氮(NH3-N)含量和化学需氧量(CODCr)较高,给该公司废水的达标排放带来很大压力.