The E3 Ligase GmPUB21 Negatively Regulates Drought and Salinity Stress Response in Soybean

E3-ubiquitin ligases are known to confer abiotic stress responses in plants. In the present study, GmPUB21, a novel U-box E3-ubiquitin ligase-encoding gene, was isolated from soybean and functionally characterized. The expression of GmPUB21, which possesses E3-ubiquitin ligase activity, was found to be significantly up-regulated by drought, salinity, and ABA treatments. The fusion protein GmPUB21-GFP was localized in the cytoplasm, nucleus, and plasma membrane. Transgenic lines of the Nicotiana benthamiana over-expressing GmPUB21 showed more sensitive to osmotic, salinity stress and ABA in seed germination and inhibited mannitol/NaCl-mediated stomatal closure. Moreover, higher reactive oxygen species accumulation was observed in GmPUB21 overexpressing plants after drought and salinity treatment than in wild-type (WT) plants. Contrarily, silencing of GmPUB21 in soybean plants significantly enhanced the tolerance to drought and salinity stresses. Collectively, our results revealed that GmPUB21 negatively regulates the drought and salinity tolerance by increasing the stomatal density and aperture via the ABA signaling pathway. These findings improved our understanding of the role of GmPUB21 under drought and salinity stresses in soybean.     

Studies on Lotus Genomics and the Contribution to Its Breeding

Lotus (Nelumbo nucifera), under the Nelumbonaceae family, is one of the relict plants possessing important scientific research and economic values. Because of this, much attention has been paid to this species on both its biology and breeding among the scientific community. In the last decade, the genome of lotus has been sequenced, and several high-quality genome assemblies are available, which have significantly facilitated functional genomics studies in lotus. Meanwhile, re-sequencing of the natural and genetic populations along with different levels of omics studies have not only helped to classify the germplasm resources but also to identify the domestication of selected regions and genes controlling different horticultural traits. This review summarizes the latest progress of all these studies on lotus and discusses their potential application in lotus breeding.     

土钉墙分步开挖中挡土机理分析

土钉墙和由它派生出的复合支护方式在实际工程中广泛应用,但在理论上对它的挡土机理研究还不够。本文对均质土中的土钉墙,采用数值分析方法,探讨开挖过程中土钉、面层、土体三者的受力变化及土钉墙的稳定机理,得到了面层受到的总土压力是主动土压力的0．05倍等一些结论。     

竞争层结构可调SOM网络在中药模式识别中的应用

针对经典SOM算法无法准确反映原始数据的特征信息,提出了竞争层结构可调的SOM算法——CSA—SOM算法。该算法增加了竞争层神经元动态调节的步骤,调节的依据是不断比较原数据的位置信息和映射后低雏空间的位置信息,使两者最终能趋于一致。因此降维后的数据能够较好地保持原数据的特征,包括距离信息、角度信息以及分布信息。该算法有效地实现了红景天药材的准确清晰分类。算法理论分析和实验结果均表明,CSA—SOM算法是一种快速、准确的数据内在规律映射可视化算法,与SOM算法相比,CSA—SOM算法的特征映射效果比较好,解决了SOM算法会使映射后数据结构发生扭曲的问题。     

基于白鲸优化算法的配电网故障恢复方法

提出了一种基于白鲸优化（BWO）算法的配电网故障恢复方法。首先,建立光储、风储系统模型以及负荷模型,以故障后重要负荷损失量最小为目标函数,进行配电网的初步孤岛划分。其次,以网络损耗和开关操作次数加权求和最小为目标,采用BWO算法求解,获取孤岛划分与开关操作配合的故障恢复结果。然后,通过3种场景对比,验证了所提方法能够在不同故障时段获得配电网故障恢复的最优结果。最后,将BWO算法与二进制粒子群优化算法、灰狼优化算法的运行结果进行对比,验证了BWO算法寻优效果更好。     

考虑热动态的综合能源系统碳排放流建模与分析

在电力系统低碳转型背景下,传统的源侧碳排放控制难以满足碳排放责任公平分摊需求,亟需对多能源网络进行碳排放流(即碳流)追踪,明确源网荷碳排放责任。基于此,提出了热网准动态碳流模型,并对综合能源系统碳流进行建模与分析。介绍了热网静态碳流的管道计算模型;从热损耗和热延时两方面对热网管道的动态特性进行描述;在此基础上,引入热网管道储能变量刻画2种情况下热动态特性对管道和节点碳流分布的影响;进一步地,提出了热电联产机组的碳势计算方法和储能设备各时刻平均碳势的计算方法,并总结了电、热网络碳流联合分析方法;通过算例分析了静态和准动态热网模型下综合能源系统的碳流分布结果,并考虑了负荷热惯性及机组碳势对碳流分布的影响。     

一种基于嵌入式平台的非接触式心率监测系统研究

心率的长期监测对心血管疾病的预防和治疗具有重要意义。当前心率检测常用的监护仪、心电图机、智能手表和运动手环等均属于接触式测量装置,长期佩戴易产生压痕,甚至给使用者带来不适。在非接触测量方面,远程光电容积脉搏波描记法(remote photoplethysmography, rPPG)可以通过分析面部视频获取心率,是一种很有潜力的心率长期监测方法。目前,绝大多数对于rPPG的研究都在使用电脑做数据分析,体积过大不易摆放,难以满足医学临床和家庭日常使用的需求。针对这一问题,本文尝试在嵌入式平台上依据rPPG原理实现心率监测。监测系统主要由树莓派4B开发板、相机和触摸屏组成。采用AdaBoost算法实现人脸识别与追踪,选取额头和脸颊作为感兴趣区域(range of interest, ROI),利用巴特沃斯带通滤波去噪,根据POS模型提取BVP波形,对来自不同ROI的BVP波形做盲源分离得到最终的脉搏波,最后利用能谱分析计算心率。实验结果表明本文所研究的系统具有与PC端相同的心率检测准确性和鲁棒性。本文的研究成果可以为心率长期监测设备的小型化和普及做出自己的贡献,也可以为智慧医疗中的远程监测...     

吉林省降水量演变情势分析

本文对吉林省降水量系列进行了深入的分析,通过对全省降水量不同空间、不同时间序列的分布规律研究计算分析,提出了吉林省降水量的演变情势.     

The research on floe ice force acting on the"Xue Long"icebreaker based on synthetic ice test and virtual mass numerical method

When the ice-going ships advance in the polar region,the navigation in the floe ice region is often encountered,the hull will suffer the high-frequency contact ...     

8605运输巷复合顶板巷道稳定性控制研究

为探究高应力情况下复合顶板的治理控制方式,以煤峪口矿8605工作面复合顶板巷道为工程背景,研究了高应力复合顶板的变形破坏机理,并确定采用“帮顶同治”的围岩控制策略.现场应用实践表明,8605工作面运输巷表面位移变化稳定时间长,锚杆索受力平稳,巷道完整性控制效果好,能够满足矿井生产技术需要.