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81.
2008年29届奥运会在北京举行,使得有关奥运的话题成为热门中的热门,与运动有关或无关的众多品牌、厂商对北京奥运年均寄予厚望,为赢取更大的收获和提升品牌知名度作最大的努力。其实早在几年前,国内外运动鞋品牌企业就已经大张旗鼓,在产品研发与广告宣传等多方面投入重金,为在这次北京奥运年带来的难得契机中处于市场制高点或明或隐地博杀着。 相似文献
82.
83.
The effects of key parameters on the preparation and regeneration of protoplast from the β-carotene-producing fungus Blakeslea trispora were discussed in this paper, including the combination of various enzymes, mycelial age, digesting time and temperature,
pH value, osmotic stabilizers, pretreatment, culture medium and culture method. Under the condition of mixed enzymes in osmotic
stabilizer (0.6 M NaCl) combined with 2% lysozyme, 3% cellulase and 3% snailase, the highest protoplast yield, as high as
7.48×106 protoplasts/mL, was obtained when mycelial age was 60 h at pH 5.0–6.0 with digesting for 14–16 h at 28 °C. After
purification of the obtained protoplasts, they were regenerated in PDA regenerative medium using bilayer plate culture method.
To validate the usability of the protoplasts, a novel plasmid with green fluorescent protein (GFP) was used in transformation
for easy visual observation. The results showed that the protoplasts prepared by the optimized method were active and applicable
in further gene manipulation experiments.
This work was presented at 13
th
YABEC symposium held at Seoul, Korea, October 20–22, 2007. 相似文献
84.
Qingzhao Yao Yuming Zhou Yanqing Sun Xiaoyun Ye 《Journal of Inorganic and Organometallic Polymers and Materials》2008,18(4):477-484
TiO2 hybrid molecular imprinted polymer (MIP) for ethofumesate using methacrylic acid (MAA) as the functional monomer and silane
coupling agent 3-(trimethoxysilyl) propylmethacrylate (KH570) as organic–inorganic connective bridge was synthesized via photo-excitation
method. Hydrogen bond was proved to act between MAA and ethofumesate for pre- and post-polymerization binding properties as
testified by UV spectrometric method. KH570 modified TiO2 nanoparticles were prepared via sonochemical reaction, which can accelerate hydrolysis, increase collision chance for the
reactive system and improve the dispersion of the nanoparticles. Scanning electron microscope (SEM), transmission electron
microscope (TEM), binding and the adsorption kinetics experiments as well as thermogravimetric analysis (TGA) were employed
for characterization. The results indicated that the hybrid MIP revealed a larger surface area and more ordered imprinting
cavities with improved thermal stability compared to organic-only MIP. Furthermore, faster adsorption kinetics and enhancive
adsorption capacity were achieved, which made it promising in chemical sensor applications. 相似文献
85.
We report the growth of highly C-axis orientation of Sr
x
Ba1−x
Nb2O6 (SBN) thin films on p-type (100) Si substrates by using a potassium-substituted SBN template layer with the sol–gel method. The crystallization of SBN thin films was found closely related to the potassium ion doping concentration and the postannealing temperature of the K-SBN template layer. Secondary ion mass spectrometry analysis showed that potassium elements were accumulated at the interface of the template layer and silicon substrate. SBN films postannealed at 750 °C with K-SBN template layer has a smaller full width at half maximum of X-ray rocking curve of 2.45° than that of 5.40° for the pure SBN films. By introducing a template layer, the surface morphologies of SBN films fabricated on silicon substrate were greatly improved. 相似文献
86.
对在五坐标数控加工中心上铣制三元流叶轮时出现的叶轮附面尺寸误差问题,进行了详细分析,并提出了问题的解决方法,有效地避免了加工中产生的超差。 相似文献
87.
88.
89.
简单介绍了广州地铁二号线民用移动通信工程概况,并就地铁民用移动通信系统引入地铁的主要技术难点进行了论述。 相似文献
90.