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101.
Chitosan chemistry and pharmaceutical perspectives 总被引:44,自引:0,他引:44
102.
103.
Tillman JA Lu F Goddard LM Donaldson ZR Dwinell SC Tittiger C Hall GM Storer AJ Blomquist GJ Seybold SJ 《Journal of chemical ecology》2004,30(12):2459-2494
104.
In a study of pigs slaughtered at British abattoirs, approximately 23% carried Salmonella in their cecal (large intestine) contents. The most frequent serotype was Salmonella Typhimurium (STM), which was the second most common cause of human salmonellosis in Great Britain. A pig industry-monitoring program was developed to reduce Salmonella infection on British farms. The control of STM infection on the farm requires an understanding of STM transmission dynamics within the herd, and a mathematical model has been developed for an infected grower-finisher farm. The model estimates the probability of a random pig being infected with STM. There are three broad categories of STM infection in pigs: pigs that are infected but unable to transmit the infection (latent); pigs that are infectious, i.e., able to transmit the infection (shedders); and pigs that have stopped shedding but harbor STM in their internal organs (carriers). The model estimates that 21.0% (5th and 95th percentiles, 0.05 to 77.5%) of slaughter-age pigs on an infected farm are likely to be shedding STM. Although this range is wide, it is biologically plausible. Sensitivity analysis of the total number of infected pigs revealed that the most significant input parameters are the probability of effective contact between a specific infectious and susceptible pig and the duration of shedding. The model predicted that 11.5% of pigs would be shedding STM at slaughter age. This value is close to the estimate obtained from a British abattoir survey that 11. 1% of pigs carried STM in their ceca, indicating that the model has reasonable validity. 相似文献
105.
The tensile strength of 576 pieces of white line horn collected over 6 mo from 14 dairy cows restricted to parity 1 or 2 was tested. None of the cows had ever been lame. Seven cows were randomly assigned to receive 20 mg/d biotin supplementation, and 7 were not supplemented. Hoof horn samples were taken from zones 2 and 3 (the more proximal and distal sites of the abaxial white line) of the medial and lateral claws of both hind feet on d 1 and on 5 further occasions over 6 mo. The samples were analyzed at 100% water saturation. Hoof slivers were notched to ensure that tensile strength was measured specifically across the white line region. The tensile stress at failure was measured in MPa and was adjusted for the cross-sectional area of the notch site. Data were analyzed in a multilevel model, which accounted for the repeated measures within cows. All other variables were entered as fixed effects. In the final model, there was considerable variation in strength over time. Tensile strength was significantly higher in medial compared with lateral claws, and zone 2 was significantly stronger than zone 3. Where the white line was visibly damaged the tensile strength was low. Biotin supplementation did not affect the tensile strength of the white line. Results of this study indicate that damage to the white line impairs its tensile strength and that in horn with no visible abnormality the white line is weaker in the lateral hind claw than the medial and in zone 3 compared with zone 2. The biomechanical strength was lowest at zone 3 of the lateral hind claw, which is the most common site of white line disease lameness in cattle. 相似文献
106.
Baylis CL MacPhee S Robinson AJ Griffiths R Lilley K Betts RP 《International journal of food microbiology》2004,96(1):35-48
To date, the survival of Escherichia coli O157:H7 and other verocytotoxin-producing E. coli (VTEC) in chocolate and other confectionery products has not been fully established, unlike Salmonella, which have been responsible for occasional outbreaks of infection linked to contaminated chocolate and related products, although none of these outbreaks have been related to products produced in the United Kingdom. The United Kingdom Biscuit, Cake, Chocolate and Confectionery Alliance commissioned this study to obtain information on the decline and potential survival of E. coli, particularly verocytotoxin-producing strains, in reduced aw confectionery products chocolate, biscuit cream and mallow. These products were artificially contaminated with high (4 log10 cfu/g) and low (2 log10 cfu/g) levels of E. coli O157:H7, O111:H- and O26:H11 and their survival, as affected by storage temperature (10, 22 and 38 degrees C), was monitored over 12 months. Preliminary studies to establish the best inoculation and recovery procedures indicated that differences between counts on selective and non-selective media used were not sufficiently different to influence the outcome of this study. Irrespective of sample type, rapid decline was observed in products stored at 38 degrees C and increased survival occurred in products stored at 10 degrees C. In chocolate (average aw 0.40), these bacteria were detected for up to 43 days in samples stored at 38 degrees C. At 22 degrees C they survived for up to 90 days and in product stored at 10 degrees C they could still be detected after 366 days storage. In biscuit cream (average aw 0.75) they survived for 2 days at 38 degrees C, 42 days at 22 degrees C and 58 days at 10 degrees C. Whilst mallow (aw ca. 0.73) was not stored at 38 degrees C, these bacteria could still be detected in samples stored for up to 113 and 273 days at 22 and 10 degrees C, respectively. The observed prolonged survival of these bacteria under conditions of reduced aw and lowered storage temperature in this study is supported by previous studies with Salmonella and E. coli O157:H7 in other foods. In the same way that Salmonella bacteria can survive for long periods, in excess of 12 months, in chocolate, this study provides evidence that E. coli, including pathogenic strains, can also survive for similar periods of time. Assuming the routes of transmission are similar, controls currently used by the confectionery industry to prevent contamination by Salmonella should also be effective against E. coli, including VT-producing strains, providing that all raw materials have been suitably processed, stored and handled before and during manufacture. 相似文献
107.
Moretti M Prina-Mello A Reid AJ Barron V Prendergast PJ 《Journal of materials science. Materials in medicine》2004,15(10):1159-1164
Endothelial cells at the interface between the bloodstream and the vessel wall are continuously subjected to mechanical stimulation in vivo, and it widely recognised that such stimulation plays an important role in cardiovascular physiology. Cell deformation is induced by mechanical forces such as cyclic stretch, fluid shear stress, and transmural pressure. Although much of the work in this field has dealt with the effect of fluid shear stress, very little is known about how cyclic forces modulate and alter the morphology of single endothelial cells, and thereafter, how they effect the confluent layer of endothelial cells lining the vessel wall. The aim of this study is to investigate the response of endothelial cells when subjected to substrate deformation of similar magnitude to those experienced in vivo. Human umbilical vein endothelial cells (HUVEC) were cultured on plasma-treated silicone strips and uni-axially cyclically stretched using a custom made mechanical device. Results showed that endothelial cells subject to 10% deformation for as little as 4 h reoriented perpendicular to the stretch direction. In addition, although no integrin coating was applied to the substrate, it was found that plasma-treated silicone provided a cell adhesion substrate comparable to the commonly used collagen type I. Thus the results show that the stretch stimulus alone affects the morphology of endothelial cells. Further studies are required to establish the relative importance of substrate strain vs. fluid flow stimuli. 相似文献
108.
Design, synthesis and properties of a degradable polyurethane scaffold for meniscus regeneration 总被引:5,自引:0,他引:5
Heijkants RG van Calck RV De Groot JH Pennings AJ Schouten AJ van Tienen TG Ramrattan N Buma P Veth RP 《Journal of materials science. Materials in medicine》2004,15(4):423-427
Longitudinal lesions in menisci are among the most frequent orthopedic problems of the knee. Repair by simple techniques is only limited to the vascular part of the meniscus. For repair of the avascular part of the meniscus a scaffold, which will assist the body in the formation of new meniscus cell tissue, might be applicable. In this study a biomedical segmented polyurethane with poly(epsilon-caprolactone) as soft segment and 1,4-butanediisocyanate and 1,4-butanediol as uniform hard segments has been synthesised. The material has a micro phase separated morphology and excellent mechanical properties. A porous scaffold was prepared via a combination of liquid-liquid phase separation and salt leaching. The foams prepared combined a very high interconnectivity and porosity with the desired compression modulus. After six months of implantation in the knees of beagles full ingrowth with cells was obtained and it was found that meniscus like tissue had been formed in the scaffold. Moreover, compression behaviour appeared to be comparable to native meniscus tissue. 相似文献
109.
Eigler DM Lutz CP Crommie MF Manoharan HC Heinrich AJ Gupta JA 《Philosophical transactions. Series A, Mathematical, physical, and engineering sciences》2004,362(1819):1135-1147
We discuss two examples of novel information-transport and processing mechanisms in nanometre-scale structures. The local modulation and detection of a quantum state can be used for information transport at the nanometre length-scale, an effect we call a 'quantum mirage'. We demonstrate that, unlike conventional electronic information transport using wires, the quantum mirage can be used to pass multiple channels of information through the same volume of a solid. We discuss a new class of nanometre-scale structures called 'molecule cascades', and show how they may be used to implement a general-purpose binary-logic computer in which all of the circuitry is at the nanometre length-scale. 相似文献
110.
Comtet G Dujardin G Hellner L Lastapis M Martin M Mayne AJ Riedel D 《Philosophical transactions. Series A, Mathematical, physical, and engineering sciences》2004,362(1819):1217-1226
The electronic or quantum control of individual molecules with the scanning tunnelling microscope offers exciting perspectives on operating molecular nanomachines. This implies the use of semiconductor surfaces rather than metallic surfaces which would rapidly quench the electronic excitations. We review recent results illustrating the state of the art and the main problems which need to be solved: the choice, design and properties of functionalized organic molecules on semiconductor surfaces; the control of the inelastic electronic channels through a single molecule; and the search for well-controlled atomic-scale wide-band-gap semiconductor surfaces. 相似文献