PYRO—JET燃烧器降低NOx排放——发展趋势和实践经验

ＰＹＲＯ—ＪＥＴ燃烧器降低ＮＯ＿ｘ排放—发展趋势和实践经验［德］洪堡公司Ｃ．Ｂａｎｅｒ一、引言何以在１９７０年代末，德国洪堡公司研究的派洛其脱（ＰＹＲＯ—ＪＥＴ）燃烧器转向在回转窑烧煤？所确定的目标在于使窑由燃油在改烧煤时水泥回转窑中产生一个直而短的...     

拱顶罐的电动葫芦倒装法施工

拱顶罐的电动葫芦倒装法施工卢宽宏（安庆石化总厂建安公司安庆２４６００１）安庆石比炼油厂２台１×１０４ｍ３拱顶罐安装工程，罐内径３１．２ｍ，高１７．５ｍ，壁板由９圈带板组成，板宽１６００ｍｍ，板厚依次为２０、１８、１６、１４、１２、１０、８、６、６ｍｍ...     

流体污染控制——第四讲 过滤——截获和清除污染物

过滤器的滤材是种多孔性的物质,依靠下述两种机理(或其中一种)来截获和留住颗粒:★吸收机理——机械的方法,例如筛或者滤。★吸附机理——表面力的方法,例如静电吸附效应或者分子粘附效应。一种理想的过滤器是对混入污染物的流     

矿山设备载荷过程的标准模型

矿山设备的实际生产载荷是非稳定随机过程,这首先是由于矿山生产的特点,即采矿地质、采矿技术和组织因素综合影响的结果。这些因素对生产载荷过程的影响具有非稳定随机性质,例如在一条长壁矿房内煤的抗切强度相差3～4倍,生产中采矿设备偶然停工时间约占工作班总时间的50%。     

Automated zebrafish chorion removal and single embryo placement: optimizing throughput of zebrafish developmental toxicity screens

The potential of the developing zebrafish model for toxicology and drug discovery is limited by inefficient approaches to manipulating and chemically exposing zebrafish embryos-namely, manual placement of embryos into 96- or 384-well plates and exposure of embryos while still in the chorion, a barrier of poorly characterized permeability enclosing the developing embryo. We report the automated dechorionation of 1600 embryos at once at 4 h postfertilization (hpf) and placement of the dechorionated embryos into 96-well plates for exposure by 6 hpf. The process removed ≥95% of the embryos from their chorions with 2% embryo mortality by 24 hpf, and 2% of the embryos malformed at 120 hpf. The robotic embryo placement allocated 6-hpf embryos to 94.7% ± 4.2% of the wells in multiple 96-well trials. The rate of embryo mortality was 2.8% (43 of 1536) from robotic handling, the rate of missed wells was 1.2% (18 of 1536), and the frequency of multipicks was <0.1%. Embryo malformations observed at 24 hpf occurred nearly twice as frequently from robotic handling (16 of 864; 1.9%) as from manual pipetting (9 of 864; 1%). There was no statistical difference between the success of performing the embryo placement robotically or manually.     

Dynex: multiplex ELISA technology

Conventional enzyme-linked immunosorbent assay (ELISA) is a gold standard for screening antibodies and testing for protein or antigen presence. A significant limitation of this assay resides in the fact that only one analyte can be assessed per microplate well. Here, we describe and investigate a new technology consisting of an automated ELISA system in which up to 10 analytes can be measured within one single well, thus improving productivity, accuracy, and repeatability by reducing the amount of human labor required. Another strength of the platform is that a user can load any necessary sets/subsets of beads to perform required assays, with improved flexibility compared to manufactured-loaded arrays for multiplex analysis. We also demonstrate that this system can be used to determine the pathogenicity (i.e., presence of Shiga toxins) and serotype (i.e., Escherichia coli O157) of E. coli isolates.